SOX8 Antibody - BSA Free

Western Blot: SOX8 Antibody [NBP3-12898] - SOX8 antibody detects SOX8 protein by western blot analysis. Human tissue extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX8 antibody (NBP3-12898) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SOX8 Antibody [NBP3-12898] - SOX8 antibody detects SOX8 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOX8 protein stained by SOX8 antibody (NBP3-12898) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: SOX8 Antibody [NBP3-12898] - SOX8 antibody detects SOX8 protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SOX8 antibody (NBP3-12898) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: SOX8 Antibody [NBP3-12898] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX8 antibody (NBP3-12898) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SOX8 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SOX8. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOX8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SOX8 Antibody - BSA Free

• MGC24837
• SRY (sex determining region Y)-box 8
• transcription factor SOX-8

Background

The SOX8 gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after for

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX8