Sortilin Antibody [Unconjugated]

Western blot shows lysates of human motor cortex. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human peripheral blood monocytes were stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Sortilin was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 82 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) . This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Sortilin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Sortilin
  Ser78-Asn755
  Accession # Q99523
• Specificity: Detects human and mouse Sortilin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SORT1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sortilin Antibody [Unconjugated]

• 100 kDa NT receptor
• Glycoprotein 95
• Gp95
• Gp95LDLCQ6
• Neurotensin receptor 3
• NT3NTR3
• Ntr3
• SORT1
• sortilin 1
• Sortilin

Background

Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Human preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre- and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10 CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature human Sortilin shares 91% aa identity with mouse and rat Sortilin and 93% aa identity with dog. During murine development, sortilin is mainly expressed in the nervous system (5) where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) (6-9). ProNGF (or the NGF propeptide alone) binds sortilin with much higher affinity (Kd ~5-8 nM) than does mature NGF (Kd ~90 nM). The complex of sortilin, pro-NGF and the receptor p75 ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with pro-BDNF and BDNF (8, 9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.

1. Petersen, C.M. et al. (1997) J. Biol. Chem. 272:3599.
2. Westergaard, U.B. et al. (2004) J. Biol. Chem. 279:50221.
3. Petersen, C.M. et al. (1998) EMBO J. 18:595.
4. Nielsen, M.S. et al. (2001) EMBO J. 20:2180.
5. Hermans-Borgmeyer, I. et al. (1999) Mol. Brain Res. 65:216.
6. Mazella, J. et al. (1998) J. Biol. Chem. 273:26273.
7. Nykjaer, A. et al. (2004) Nature 427:843.
8. Teng, H.K. et al. (2005) J. Neurosci. 25:5455.
9. Chen, Z-Y. et al. (2004) J. Neurosci. 25:6156.
10. Shi, J. and K.V. Kandror (2005) Dev. Cell 9:99.
11. Nielsen, M.S. et al. (1999) J. Biol. Chem. 274:8832.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sortilin Antibody (AF3154)(9)

Publications using AF3154

• Ullgren, A;Öijerstedt, L;Olofsson, J;Bergström, S;Remnestål, J;van Swieten, JC;Jiskoot, LC;Seelaar, H;Borroni, B;Sanchez-Valle, R;Moreno, F;Laforce, R;Synofzik, M;Galimberti, D;Rowe, JB;Masellis, M;Tartaglia, MC;Finger, E;Vandenberghe, R;de Mendonça, A;Tirabosch, P;Santana, I;Ducharme, S;Butler, CR;Gerhard, A;Otto, M;Bouzigues, A;Russell, L;Swift, IJ;Sogorb-Esteve, A;Heller, C;Rohrer, JD;Månberg, A;Nilsson, P;Graff, C;Genetic Frontotemporal Dementia Initiative (GENFI), ; Altered plasma protein profiles in genetic FTD - a GENFI study Molecular neurodegeneration 2023-11-15 [PMID: 37968725] (Bioassay, Human)
• Juan Jiang, Chen Yang, Jia-Qi Ai, Qi-Lei Zhang, Xiao-Lu Cai, Tian Tu et al. Intraneuronal sortilin aggregation relative to granulovacuolar degeneration, tau pathogenesis and sorfra plaque formation in human hippocampal formation Frontiers in Aging Neuroscience 8/1/2022 [PMID: 35978952]
• Shu-Yin Xu, Qi-Lei Zhang, Qi Zhang, Lily Wan, Juan Jiang, Tian Tu et al. Regional and Cellular Mapping of Sortilin Immunoreactivity in Adult Human Brain Frontiers in Neuroanatomy 3/12/2019 [PMID: 30914927]
• C Fleitas, G Piñol-Ripo, P Marfull, D Rocandio, I Ferrer, C Rampon, J Egea, C Espinet proBDNF is modified by advanced glycation end products in Alzheimer&#039;s disease and causes neuronal apoptosis by inducing p75 neurotrophin receptor processing Mol Brain, 2018-11-14;11(1):68. 2018-11-14 [PMID: 30428894] (ICC, IHC-P, Human, Transgenic Mouse)
• S Hagita, MA Rogers, T Pham, JR Wen, AK Mlynarchik, M Aikawa, E Aikawa Transcriptional control of intestinal cholesterol absorption, adipose energy expenditure and lipid handling by Sortilin Sci Rep, 2018-06-13;8(1):9006. 2018-06-13 [PMID: 29899496] (Western Blot, Mouse)
• FQ Zhou, J Jiang, CM Griffith, PR Patrylo, H Cai, Y Chu, XX Yan Lack of human-like extracellular sortilin neuropathology in transgenic Alzheimer&#039;s disease model mice and macaques Alzheimers Res Ther, 2018-04-24;10(1):40. 2018-04-24 [PMID: 29690919] (IHC, Primate)
• Xia Hu, Zhao-Lan Hu, Zheng Li, Chun-Sheng Ruan, Wen-Ying Qiu, Aihua Pan et al. Sortilin Fragments Deposit at Senile Plaques in Human Cerebrum Frontiers in Neuroanatomy 2017-06-07 [PMID: 28638323]
• Xia Hu, Zhao-Lan Hu, Zheng Li, Chun-Sheng Ruan, Wen-Ying Qiu, Aihua Pan et al. Sortilin Fragments Deposit at Senile Plaques in Human Cerebrum Frontiers in Neuroanatomy 2017-06-07 [PMID: 28638323]
• Fauchais AL, Lalloue F, Lise MC, Boumediene A, Preud'homme JL, Vidal E, Jauberteau MO Role of endogenous brain-derived neurotrophic factor and sortilin in B cell survival. J. Immunol., 2008-09-01;181(5):3027-38. 2008-09-01 [PMID: 18713973] (ICC, Neutralization, Western Blot, Human)

Review for Sortilin Antibody (AF3154) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer IF Other 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22579764
• Species: Other

Bioinformatics

• Gene Symbol: SORT1
• Uniprot:
  • Q99523()