Sortilin Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Sortilin Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Sortilin
  Ser78-Asn755
  Accession # Q99523
• Specificity: Detects human and mouse Sortilin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SORT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sortilin Antibody [Biotin]

• 100 kDa NT receptor
• Glycoprotein 95
• Gp95
• Gp95LDLCQ6
• Neurotensin receptor 3
• NT3NTR3
• Ntr3
• SORT1
• sortilin 1
• Sortilin

Background

Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Human preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre‑ and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10 CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature human Sortilin shares 91% aa identity with mouse and rat Sortilin and 93% aa identity with dog. During murine development, sortilin is mainly expressed in the nervous system (5) where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain‑derived neurotrophic factor (BDNF) (6-9). ProNGF (or the NGF propeptide alone) binds sortilin with much higher affinity (K_D ~5-8 nM) than does mature NGF (K_D ~90 nM). The complex of sortilin, pro-NGF and the receptor p75^ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with pro-BDNF and BDNF (8, 9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.

1. Petersen, C.M. et al. (1997) J. Biol. Chem. 272:3599.
2. Westergaard, U.B. et al. (2004) J. Biol. Chem. 279:50221.
3. Petersen, C.M. et al. (1998) EMBO J. 18:595.
4. Nielsen, M.S. et al. (2001) EMBO J. 20:2180.
5. Hermans-Borgmeyer, I. et al. (1999) Mol. Brain Res. 65:216.
6. Mazella, J. et al. (1998) J. Biol. Chem. 273:26273.
7. Nykjaer, A. et al. (2004) Nature 427:843.
8. Teng, H.K. et al. (2005) J. Neurosci. 25:5455.
9. Chen, Z-Y. et al. (2004) J. Neurosci. 25:6156.
10. Shi, J. and K.V. Kandror (2005) Dev. Cell 9:99.
11. Nielsen, M.S. et al. (1999) J. Biol. Chem. 274:8832.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sortilin Antibody (BAF3154)(2)

Publications using BAF3154

• Kurnellas, M;Mitra, A;Schwabe, T;Paul, R;Arrant, AE;Roberson, ED;Ward, M;Yeh, F;Long, H;Rosenthal, A; Latozinemab, a novel progranulin-elevating therapy for frontotemporal dementia Journal of translational medicine 2023-06-15 [PMID: 37322482] (ELISA Detection, Transgenic Mouse, Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• S Miyakawa, H Sakuma, D Warude, S Asanuma, N Arimura, T Yoshihara, D Tavares, A Hata, K Ida, Y Hori, Y Okuzono, S Yamamoto, K Iida, H Shimizu, S Kondo, S Sato Anti-sortilin1 Antibody Up-Regulates Progranulin via Sortilin1 Down-Regulation Frontiers in Neuroscience, 2020-12-15;14(0):586107. 2020-12-15 [PMID: 33384578] (ICC, Human)

Bioinformatics

• Gene Symbol: SORT1
• Uniprot:
  • Q99523()