Sonic Hedgehog/Shh Antibody - BSA Free

Western Blot: Sonic Hedgehog Antibody [NBP2-22139] - WB detection of Sonic Hedgehog in A. human stomach, B. human fetal liver membrane, C. PC12 and D. Cos7

Immunocytochemistry/Immunofluorescence: Sonic Hedgehog Antibody [NBP2-22139] - Sonic Hedgehog antibody was tested in SH-SY5Y cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: Sonic Hedgehog Antibody [NBP2-22139] - IHC analysis of Sonic Hedgehog in mouse kidney.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Sonic Hedgehog/Shh Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Sonic Hedgehog/Shh Antibody - BSA Free (NBP2-22139) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Sonic Hedgehog/Shh Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an N-terminal portion of the human SHH protein (between residues 1-75) [UniProt Q15465]
• Localization: Cytoplasm. Membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Western Blot 1-2 ug/ml
• Application Notes: This SHH antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band is observed at ~50 kDa. In Immunocytochemistry/Immunofluorescence cytoplasm/membrane staining was observed in SH-SY5Y cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  Human Stomach Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Sonic Hedgehog/Shh Peptide (NBP2-22139PEP)

Reactivity Notes

Chicken (83%), Zebrafish (84%), Xenopus (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sonic Hedgehog/Shh Antibody - BSA Free

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Sonic Hedgehog (SHH), also known as HLP3, HPE3, MCOPCB5, SMMC1, and HHG1, is a gene that encodes for a protein instrumental in patterning the early embryo. Specifically, SHH has been implicated as the main signal in mapping out the development of the ventral neural tube, the anterior-posterior limb access, and the ventral somites. This occurs by SHH binding to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. SHH also regulates another target gene, the gli oncogene. In adults, SHH helps to control stem cell proliferation. The N-terminal form of SHH is associated with lipid rafts at the cell surface, while on a tissue level SHH is expressed in fetal intestine, liver, lung and kidney. Recent research has found SHH to modulate the spatial organization of neuroepithelial cell proliferation in the embryonic chick (PMID: 23031710), as well as detected a role for SHH in Idiopathic Pulmonary Fibrosis (PMID: 23023967).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sonic Hedgehog/Shh Antibody (NBP2-22139)(5)

Publications using NBP2-22139

• Li B, Yan YP, He YY et al. IHH, SHH, and primary cilia mediate epithelial-stromal cross-talk during decidualization in mice Science signaling 2023-02-28 [PMID: 36853961]
• Yi C, Spitters TW, Al-Far EAA et al. A calcineurin-mediated scaling mechanism that controls a K(+)-leak channel to regulate morphogen and growth factor transcription eLife 2021-04-08 [PMID: 33830014] (Mouse)
• Tessier S, Madhu V, Johnson ZI et al. NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis Dev. Biol. 2019-07-10 [PMID: 31301300] (IHC-P, Mouse)
• Morales-Hernandez A, Nacarino-Palma A, Moreno-Marin N et al. Lung regeneration after toxic injury is improved in absence of dioxin receptor. Stem Cell Research. [PMID: 29107893] (ICC/IF, Mouse)
• Palacio-Mancheno PE, Evashwick-Rogler TW, Laudier DM et al. Hyperosmolarity induces notochordal cell differentiation with aquaporin3 upregulation and reduced N-cadherin expression J. Orthop. Res. 2017-08-29 [PMID: 28853179] (Mouse)

Bioinformatics

• Gene Symbol: SHH
• Entrez:
  • 6469(Human)
  • 20423(Mouse)
  • 29499(Rat)
• Uniprot:
  • Q15465()
  • Q62226()
  • Q63673(Rat)