SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 . Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteo-arthritis..

Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 . Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000.

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4GG11G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SOD3/EC-SOD Antibody (4GG11G6) - BSA Free Summary

• Immunogen: Human extracellular SOD purified from aortas
• Localization: Extracellular Space
• Specificity: Detects extracellular SOD approx 35kDa.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOD3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of SOD (EC) Antibody was sufficient for detection of EC-SOD in 20 ug of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

• ECSOD
• EC-SOD
• EC-SODEC 1.15.1.1
• extracellular superoxide dismutase [Cu-Zn]
• MGC20077
• SOD3
• superoxide dismutase 3, extracellular

Background

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOD3/EC-SOD Antibody (NBP1-22417)(3)

Publications using NBP1-22417

• Davis SM, Collier LA, Foran EA et al. Neuroprotective activity of leukemia inhibitory factor is relayed through myeloid zinc finger-1 in a rat model of stroke Metab Brain Dis. 2019-04-15 [PMID: 30612292] (IF/IHC, Rat)
  
  Details:
  Citation using the HRP form of this antibody.
• Davis SM, Collier LA, Leonardo CC et al. Leukemia Inhibitory Factor Protects Neurons from Ischemic Damage via Upregulation of Superoxide Dismutase 3. Mol. Neurobiol. 2016-01-09 [PMID: 26746670] (WB, IF/IHC, Rat)
• Wang Ca, Harrell J, Iwanaga R et al. Vascular endothelial growth factor-C promotes breast cancer progression via a novel anti-oxidant mechanism that involves regulation of Superoxide dismutase 3 Breast Cancer Res. 2014-10-30 [PMID: 25358638] (WB, Human, Mouse)
  
  Details:
  SOD3 antibody used for WB on lysates of 66 cl4 mammary carcinoma cells subjected or not to VEGF-C knockdown (Figure 5b). Antibody also used in WB for detecting secreted Sod3 in the media of 66 cl4-scram, 66 cl4-VEGF-C KD and 66 cl4-VEGF-C KD + Sod3 cells (Figure 6a), and in totall cell lysates as well as the medium from 66 cl4-scram and 66 cl4-nrp2 knockdown cells (Figure 7B). WB with SOD3 also performed on MDA-MB-231 breast cancer cells subjected or not to VEGF-C knockdown (Figure S8).

FAQs for SOD3/EC-SOD Antibody (NBP1-22417). (Showing 1 - 1 of 1 FAQs).

1. I've been using your mouse monoclonal anti-SOD3 antibody for a number of applications and have been very pleased with the results. However, I want to know what would be a good positive control for WB. The datasheet says that human aorta was used as the immunogen. However, I read that lung and kidney lysates make good positive controls for other anti-SOD3 antibodies. Would lung/kidney tissue work as a positive control for this particular antibody?
   • SOD3 protein is mainly expressed in blood vessels, heart, lung, kidney and placenta, and it is the major SOD isoenzyme found in extracellular fluids such as plasma, lymph and synovial fluid. So using lung or kidney as a positive control lysate would be appropriate for your immunoassays including Western blot.

Bioinformatics

• Gene Symbol: SOD3
• Entrez:
  • 6649(Human)
• Uniprot:
  • P08294()