Western Blot: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis using SOD1 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 ...read more
Flow Cytometry: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of A431 cells using SOD1 mouse mAb (green) and negative control (purple).
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of 3T3-L1 cells using SOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP1-47443 in the following applications:
Read Publications using NBP1-47443 in the following applications:
Fish reactivity reported in scientific literature (PMID: 26965749). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD1), mitochondrial manganese SOD (Mn-SOD/SOD2) and extracellular Cu, Zn-SOD (EC-SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32-kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80-kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Choi YJ, Choi JY, Yang SG, Kim BS. The effect of green and red light spectra and their intensity on the oxidative stress and non-specific immune responses in gold-striped amberjack, Seriola lalandi. Marine and Freshwater Behaviour and Physiology. 2016 Apr 07 (WB, Fish)
Details: SOD antibody was used at 1:2000 dilution for WB analysis of total protein isolated from the liver of gold-striped amberjack (Seriola lalandi). The antibody detected specific band of superoxide dismutase/SOD at 18 kDa position (Figure 2a)
Jung MM, Kim TH, Choi YJ, Kim NN. Variations in the antioxidant system and circadian rhythms of goldfish, Carassius auratus, exposed to ammonia: profile of the effects of green LED spectra. Biological Rhythm Research. 42464 (WB, Fish)
Details: SOD antibody was used at 1:4000 dilution for WB analysis of total protein isolated from the liver of goldfish, Carassius auratus. The antibody detected specific band of superoxide dismutase/SOD at 18 kDa position (Figure 2a).
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