SOD1/Cu-Zn SOD Antibody (6F5)

Images

 
Western Blot: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis using SOD1 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 ...read more
Flow Cytometry: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of A431 cells using SOD1 mouse mAb (green) and negative control (purple).
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of 3T3-L1 cells using SOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Product Details

Summary
Reactivity Hu, Mu, FiSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
6F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SOD1/Cu-Zn SOD Antibody (6F5) Summary

Immunogen
Purified recombinant fragment of human SOD1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SOD1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-47443 in the following applications:

Publications
Read Publications using
NBP1-47443 in the following applications:

  • WB
    5 publications

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 26965749). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for SOD1/Cu-Zn SOD Antibody (6F5)

  • ALS
  • ALS1
  • amyotrophic lateral sclerosis 1 (adult)
  • Cu
  • Cu/Zn superoxide dismutase
  • CuZn SOD
  • Cu-Zn SOD
  • EC 1.15.1.1
  • homodimer
  • hSod1
  • indophenoloxidase A
  • Ipo1
  • IPOA
  • SOD
  • SOD, cytosolic
  • SOD, Soluble
  • SOD1
  • superoxide dismutase [Cu-Zn]
  • Superoxide dismutase 1
  • superoxide dismutase 1, soluble
  • Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD1), mitochondrial manganese SOD (Mn-SOD/SOD2) and extracellular Cu, Zn-SOD (EC-SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32-kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80-kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOD1/Cu-Zn SOD Antibody (NBP1-47443)(7)

We have publications tested in 2 confirmed species: Human, Fish.

We have publications tested in 1 application: WB.


Filter By Application
WB
(5)
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Human
(1)
Fish
(3)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-47443 Applications Species
Kim BS, Jung SJ, Choi YJ et al. Effects of different light wavelengths from LEDs on oxidative stress and apoptosis in olive flounder (Paralichthys olivaceus) at high water temperatures. Fish Shellfish Immunol. Jun 15 2016 [PMID: 27320868] (WB) WB
Choi JY, Kim TH, Choi YJ et al. Effects of various LED light spectra on antioxidant and immune response in juvenile rock bream, Oplegnathus fasciatus exposed to bisphenol A. Environ Toxicol Pharmacol 2016 Jul [PMID: 27299659]
Jung SJ, Choi YJ, Kim NN et al. Effects of melatonin injection or green-wavelength LED light on the antioxidant system in goldfish (Carassius auratus) during thermal stress. Fish Shellfish Immunol. 2016 Mar 08 [PMID: 26965749] (WB, Fish) WB Fish
Kadam PD, Chuan HH. Erratum to: Rectocutaneous fistula with transmigration of the suture: a rare delayed complication of vault fixation with the sacrospinous ligament. Int Urogynecol J 2016 Mar [PMID: 26811110]
Roma A, Ovadje P, Steckle M et al. Selective Induction of Apoptosis by Azadarichta indica Leaf Extract by Targeting Oxidative Vulnerabilities in Human Cancer Cells. J Pharm Pharm Sci. 2015 Nov 01 [PMID: 26626256] (WB, Human)

Details:
SOD1/Cu-Zn SOD antibody was used for WB analysis of lysates from Jurkat /E6-1 cells that were treated or not with aqueous or ethanolic Azadarichta indica/neem leaf extracts (Figure 7).
WB Human
Choi YJ, Choi JY, Yang SG, Kim BS. The effect of green and red light spectra and their intensity on the oxidative stress and non-specific immune responses in gold-striped amberjack, Seriola lalandi. Marine and Freshwater Behaviour and Physiology. 2016 Apr 07 (WB, Fish)

Details:
SOD antibody was used at 1:2000 dilution for WB analysis of total protein isolated from the liver of gold-striped amberjack (Seriola lalandi). The antibody detected specific band of superoxide dismutase/SOD at 18 kDa position (Figure 2a)
WB Fish
Jung MM, Kim TH, Choi YJ, Kim NN. Variations in the antioxidant system and circadian rhythms of goldfish, Carassius auratus, exposed to ammonia: profile of the effects of green LED spectra. Biological Rhythm Research. 42464 (WB, Fish)

Details:
SOD antibody was used at 1:4000 dilution for WB analysis of total protein isolated from the liver of goldfish, Carassius auratus. The antibody detected specific band of superoxide dismutase/SOD at 18 kDa position (Figure 2a).
WB Fish

Review for SOD1/Cu-Zn SOD Antibody (NBP1-47443) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-47443:
Filter by Applications
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 SOD1/Cu-Zn SOD NBP1-47443
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reviewed by:
Anonymous
Human 03/24/2017
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Summary

Sample TestedHuman SOD1 Recombinant Protein
SpeciesHuman
Lot150908

Comments

CommentsThis antibody captured 6 peptides (out of 11 possible), providing a 63% coverage of the protein, however, Novus cannot provide the concentration of this antibody, which is a problem for us.

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ICC/IF Video Protocol

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Recent Reviews

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5
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4
1
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2
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1
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Anonymous
03/24/2017
Application:
Species: Human

Bioinformatics

Gene Symbol SOD1
Entrez
Uniprot