SOD1/Cu-Zn SOD Antibody (6F5)

Western Blot: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis using SOD1 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.

Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of A431 cells using SOD1 mouse mAb (green) and negative control (purple).

Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody (6F5) [NBP1-47443] - Analysis of 3T3-L1 cells using SOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Product Discontinued

SOD1/Cu-Zn SOD Antibody (6F5) Summary

• Immunogen: Purified recombinant fragment of human SOD1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOD1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 26965749). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for SOD1/Cu-Zn SOD Antibody (6F5)

• ALS
• ALS1
• amyotrophic lateral sclerosis 1 (adult)
• Cu
• Cu/Zn superoxide dismutase
• CuZn SOD
• Cu-Zn SOD
• EC 1.15.1.1
• homodimer
• hSod1
• indophenoloxidase A
• Ipo1
• IPOA
• SOD
• SOD, cytosolic
• SOD, Soluble
• SOD1
• superoxide dismutase [Cu-Zn]
• Superoxide dismutase 1
• superoxide dismutase 1, soluble
• Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD1), mitochondrial manganese SOD (Mn-SOD/SOD2) and extracellular Cu, Zn-SOD (EC-SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32-kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80-kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOD1/Cu-Zn SOD Antibody (NBP1-47443)(10)

Publications using NBP1-47443

• Jung SJ, Choi YJ, Kim NN et al. Effects of melatonin injection or green-wavelength LED light on the antioxidant system in goldfish (Carassius auratus) during thermal stress. Fish Shellfish Immunol. 2016-03-08 [PMID: 26965749] (WB, Fish)
• Roma A, Ovadje P, Steckle M et al. Selective Induction of Apoptosis by Azadarichta indica Leaf Extract by Targeting Oxidative Vulnerabilities in Human Cancer Cells. J Pharm Pharm Sci. 2015-11-01 [PMID: 26626256] (WB, Human)
  
  Details:
  SOD1/Cu-Zn SOD antibody was used for WB analysis of lysates from Jurkat /E6-1 cells that were treated or not with aqueous or ethanolic Azadarichta indica/neem leaf extracts (Figure 7).
• Choi YJ, Choi JY, Yang SG, Kim BS. The effect of green and red light spectra and their intensity on the oxidative stress and non-specific immune responses in gold-striped amberjack, Seriola lalandi. Marine and Freshwater Behaviour and Physiology. 2016-04-07 (WB, Fish)
  
  Details:
  SOD antibody was used at 1:2000 dilution for WB analysis of total protein isolated from the liver of gold-striped amberjack (Seriola lalandi). The antibody detected specific band of superoxide dismutase/SOD at 18 kDa position (Figure 2a)
• Jung MM, Kim TH, Choi YJ, Kim NN. Variations in the antioxidant system and circadian rhythms of goldfish, Carassius auratus, exposed to ammonia: profile of the effects of green LED spectra. Biological Rhythm Research. 4246-01-01 (WB, Fish)
  
  Details:
  SOD antibody was used at 1:4000 dilution for WB analysis of total protein isolated from the liver of goldfish, Carassius auratus. The antibody detected specific band of superoxide dismutase/SOD at 18 kDa position (Figure 2a).
• Tasca G, Lattante S, Marangi G et al. SOD1 p.D12Y variant is associated with amyotrophic lateral sclerosis/distal myopathy spectrum Eur. J. Neurol. 2020-04-06 [PMID: 32250500]
• Kim BS, Jung SJ, Choi YJ et al. Effects of different light wavelengths from LEDs on oxidative stress and apoptosis in olive flounder (Paralichthys olivaceus) at high water temperatures. Fish Shellfish Immunol. 2016-06-15 [PMID: 27320868] (WB)
• Choi JY, Kim TH, Choi YJ et al. Effects of various LED light spectra on antioxidant and immune response in juvenile rock bream, Oplegnathus fasciatus exposed to bisphenol A. Environ Toxicol Pharmacol 2016-07-01 [PMID: 27299659]
• Kadam PD, Chuan HH. Erratum to: Rectocutaneous fistula with transmigration of the suture: a rare delayed complication of vault fixation with the sacrospinous ligament. Int Urogynecol J. 2016-03-01 [PMID: 26811110]
  
  Details:
  This citation used the HRP version of this antibody.
• Choi C, Song J, Choi Y et al. Effects of Green Wavelength Light on Antioxidant Responses in Olive Flounder Paralichthys olivaceus Exposed to Starvation and Re-feeding Turkish Journal of Fisheries and Aquatic Sciences 2020-06-11 (IHC-P, Fish)
  
  Details:
  Paralichthys olivaceus
• Bovio F, Sciandrone B, Urani C et al. Superoxide dismutase 1 (SOD1) and cadmium: a three models approach to the comprehension of its neurotoxic effects Neurotoxicology 2021-03-24 [PMID: 33774064] (WB)

Review for SOD1/Cu-Zn SOD Antibody (NBP1-47443) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer MSIA (Mass Spectrometry Immunoassay) Human 03/24/2017

Summary

• Application: Other
• Sample Tested: Human SOD1 Recombinant Protein
• Species: Human
• Lot: 150908

Comments

• Comments: This antibody captured 6 peptides (out of 11 possible), providing a 63% coverage of the protein, however, Novus cannot provide the concentration of this antibody, which is a problem for us.

FAQs for SOD1/Cu-Zn SOD Antibody (NBP1-47443). (Showing 1 - 1 of 1 FAQs).

1. What is the amino acid sequence of the immunogen of this mAb [SOD1/Cu-Zn SOD Antibody (6F5)]?s? 
   • The immunogen is the entire full length protein and we do not map the epitopes so that is all of the information that we have about specificity of the protein.

Bioinformatics

• Gene Symbol: SOD1
• Entrez:
  • 6647(Human)
  • 20655(Mouse)
• Uniprot:
  • P00441()