SNAP25 [p Ser187] Antibody - Azide Free

Western Blot: SNAP25 [p Ser187] Antibody [NBP2-29522] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25k SNAP25 phosphorylated at Ser187 (Control). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 400 units/100ul lysate for 30 min).

• Reactivity: Mu, Rt, Hu, Gp, Pm, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

SNAP25 [p Ser187] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit SNAP25 [p Ser187] Antibody - Azide Free (NBP2-29522) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH. Accession # P60881
• Modification: p Ser187
• Specificity: Specific for endogenous levels of the ~25 kDa SNAP25 protein phosphorylated at Ser187. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Human (100%), Primate (100%), Guinea Pig (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNAP25
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for SNAP25 [p Ser187] Antibody - Azide Free

• bA416N4.2
• dJ1068F16.2
• FLJ23079
• resistance to inhibitors of cholinesterase 4 homolog
• RIC4
• RIC-4
• SEC9
• SNAP25
• SNAP-25SNAP
• SUP
• Super protein
• Synaptosomal-associated 25 kDa protein
• synaptosomal-associated protein 25
• synaptosomal-associated protein, 25kD
• synaptosomal-associated protein, 25kDa

Background

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer's Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNAP25
• Entrez:
  • 25012(Rat)
• Uniprot:
  • P60881(Rat)