STAG2 Antibody

Images

 
Western Blot: STAG2 Antibody [NB100-300] - Detection of human and mouse SA2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. ...read more
Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-300] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

STAG2 Antibody Summary

Background
.
Immunogen
A 31 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 2 encoded within exon 31 (LocusLink ID 10735). Host: Goat A 31 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 2 encoded within exon 31 (LocusLink ID 10735).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STAG2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
Control
HeLa Nuclear Cell Lysate
Publications
Read Publications using
NB100-300 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STAG2 Antibody

  • bA517O1.1
  • cohesin subunit SA-2
  • DKFZp686P168
  • SA-2
  • SA2FLJ25871
  • SCC3 homolog 2
  • stromal antigen 2DKFZp781H1753

Background

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAG2 Antibody (NB100-300)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STAG2