SMAC/Diablo Antibody

Images

 
Western Blot: SMAC/Diablo Antibody [NB100-56165] - Western blot analysis of extracts from 293 and HeLa cell lines using Smac antibody at dilution of 1:1000. Image from verified customer review,
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56165] - IHC analysis of paraffin-embedded human lung carcinoma using Smac antibody at dilution of 1:25. Image from verified customer review.
Western Blot: SMAC/Diablo Antibody [NB100-56165] - Western blot analysis of MCF7 (A) and K562 (B) cell lysate using Smac (NB100-56165) antibody at 1:500.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56165] - Formalin-fixed, paraffin-embedded tissue cores from a human breast cancer microarray stained for Smac/Diablo using NB100-56165 at 1:2000. ...read more
Simple Western: SMAC/Diablo Antibody [NB100-56165] - Simple Western lane view shows a specific band for Smac in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GeSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SMAC/Diablo Antibody Summary

Immunogen
Full-length recombinant human SMAC was used as immunogen.
Specificity
Smac
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIABLO
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Simple Western 1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-56165 in the following applications:

Reactivity Notes

Cross reacts with Gerbil, Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for SMAC/Diablo Antibody

  • 0610041G12Rik
  • Diablo
  • diablo, IAP-binding mitochondrial protein
  • Direct IAP-binding protein with low pI
  • mitochondrial Smac protein
  • Second mitochondria-derived activator of caspase
  • SMAC
  • SMACmitochondrial

Background

Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria (reviewed in Ren et al, 2006; Shiozaki and Shi, 2004). During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways. However, the expression and role of Smac/DIABLO in primary human tumors remains to be fully elucidated. Several alternatively spliced transcript variants that encode distinct SMAC isoforms have been described, including isoform 1 (approx. 27 kDa), isoform 2 (approx. 22 kDa) and isoform 3 (approx. 21 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMAC/Diablo Antibody (NB100-56165) (0)

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Reviews for SMAC/Diablo Antibody (NB100-56165) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: IHC-P, WB.

Reviews using NB100-56165:
Filter by Applications
IHC-P
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin SMAC/Diablo NB100-56165
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reviewed by:
Anony- mous
IHC-P Human 08/29/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
SpeciesHuman
Special ApplicationsNB100-56165 Smac antibody detected endogenous level of Smac in human lung carcinoma in IHC at dilution of 1:25.
FileView PDF
Western Blot SMAC/Diablo NB100-56165
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reviewed by:
Anony- mous
WB Human 08/29/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsNB100-56165 Smac antibody detected endogenous level of Smac in 293 and HeLa cell lysates in WB at dilution of 1:1000.
FileView PDF

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Video Protocols

WB Video Protocol

FAQs for SMAC/Diablo Antibody (NB100-56165) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for SMAC/Diablo Antibody (NB100-56165)

Discover related pathways, diseases and genes to SMAC/Diablo Antibody (NB100-56165). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SMAC/Diablo Antibody (NB100-56165)

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Research Areas for SMAC/Diablo Antibody (NB100-56165)

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Blogs on SMAC/Diablo.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

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Recent Reviews

5
5
2
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2
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1
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Anony- mous
08/29/2014
Application: IHC-P
Species: Human

Anony- mous
08/29/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol DIABLO
Entrez