SMAC/Diablo Antibody - BSA Free

Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of extracts from 293 and HeLa cell lines using this antibody at dilution of 1:1000. Image from verified customer review,

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of paraffin-embedded human lung carcinoma using this antibody at dilution of 1:25. Image from verified customer review.

Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of MCF7 (A) and K562 (B) cell lysate using this antibody at 1:500.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Formalin-fixed, paraffin-embedded tissue cores from a human breast cancer microarray stained for Smac/Diablo using this antibody at 1:2000. Hematoxylin-eosin counterstain. Differential Smac/DIABLO staining is seen between patient samples.

Simple Western: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Shows a specific band for Smac in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SMAC/Diablo Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMAC/Diablo Antibody - BSA Free (NB100-56165) is a polyclonal antibody validated for use in IHC, WB, Simple Western and IP. Anti-SMAC/Diablo Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length recombinant human SMAC was used as immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIABLO
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Simple Western 1:400
  • Western Blot 1:1000-1:2000
• Application Notes: Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:400, apparent MW was 28 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Cross reacts with Gerbil

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for SMAC/Diablo Antibody - BSA Free

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria (reviewed in Ren et al, 2006; Shiozaki and Shi, 2004). During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways. However, the expression and role of Smac/DIABLO in primary human tumors remains to be fully elucidated. Several alternatively spliced transcript variants that encode distinct SMAC isoforms have been described, including isoform 1 (approx. 27 kDa), isoform 2 (approx. 22 kDa) and isoform 3 (approx. 21 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMAC/Diablo Antibody (NB100-56165)(1)

Publication using NB100-56165

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Reviews for SMAC/Diablo Antibody (NB100-56165) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Bryan Tinsley IHC-P Human 08/29/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Species: Human

reviewed by: Bryan Tinsley WB Human 08/29/2014

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: DIABLO
• Entrez:
  • 66593(Mouse)
  • 288753(Rat)