Sirtuin 3/SIRT3 Antibody (S03-1F5)

Western Blot: Sirtuin 3/SIRT3 Antibody (S03-1F5) [NBP3-15091] - Western blot detection of Sirtuin 3/SIRT3 in Jurkat, Hela cell lysates using Sirtuin 3/SIRT3 Rabbit mAb (1:500 diluted). Predicted band size: 44kDa. Observed band size: 28kDa.

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (S03-1F5) [NBP3-15091] - Immunohistochemistry of Sirtuin 3/SIRT3 in paraffin-embedded Human breast cancer tissue using Sirtuin 3/SIRT3 Rabbit mAb at dilution 1/50

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S03-1F5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Sirtuin 3/SIRT3 Antibody (S03-1F5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Sirtuin 3/SIRT3 (Uniprot#: Q9NTG7)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIRT3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin 10ug/ml
  • Western Blot 1/1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Sirtuin 3/SIRT3 Antibody (S03-1F5)

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT3