SF-1/NR5A1/Steroidogenic Factor 1 Antibody (JE33-89)

Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (JE33-89) [NBP3-32982] - Western blot analysis of SF-1/NR5A1/Steroidogenic Factor 1 on different lysates with Rabbit anti-SF-1/NR5A1/Steroidogenic Factor 1 antibody (NBP3-32982) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat ovary tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32982) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE33-89
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (JE33-89) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human SF-1/NR5A1/Steroidogenic Factor 1 aa 312-461 / 461. (Uniprot: Q13285)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NR5A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (JE33-89)

• AD4BP
• AD4BPSTF-1
• Adrenal 4-binding protein
• ELP
• FTZ1adrenal 4 binding protein
• FTZF1
• FTZF1nuclear receptor AdBP4
• Fushi tarazu factor homolog 1
• NR5A1
• Nuclear receptor subfamily 5 group A member 1
• nuclear receptor subfamily 5, group A, member 1
• SF1
• SF-1
• SF-1POF7
• SF1steroidogenic factor 1
• Steroid hormone receptor Ad4BP
• steroidogenic factor-1

Background

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR5A1