Serpin F1/PEDF Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human breast tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Serpin F1/PEDF at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin F1/PEDF at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Serpin F1/PEDF was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 56 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin F1/PEDF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Serpin F1/PEDF
  Asp44-Pro418
  Accession # P36955
• Specificity: Detects human Serpin F1/PEDF in direct ELISAs. Detects human and mouse Serpin F1/PEDF in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2-2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin F1/PEDF Antibody [Unconjugated]

• Cell proliferation-inducing gene 35 protein
• EPC-1
• EPC-1PIG35
• PEDF
• PEDFpigment epithelium-derived factor
• pigment epithelium derived factor), member 1
• proliferation-inducing protein 35
• serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin
• Serpin F1
• serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Background

Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human Serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387‑411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44‑418, human Serpin F1 shares 87% aa identity with mouse Serpin F1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin F1/PEDF Antibody (AF1177)(9)

Publications using AF1177

• Sei Kuriyama, Gentaro Tanaka, Kurara Takagane, Go Itoh, Masamitsu Tanaka Pigment Epithelium Derived Factor Is Involved in the Late Phase of Osteosarcoma Metastasis by Increasing Extravasation and Cell-Cell Adhesion Frontiers in Oncology 2022-01-31 [PMID: 35174090]
• V Vigneswara, Z Ahmed Pigment epithelium-derived factor mediates retinal ganglion cell neuroprotection by suppression of caspase-2 Cell Death Dis, 2019-02-04;10(2):102. 2019-02-04 [PMID: 30718480] (Agonist Activity, Rat)
• E Melo, P Oertle, C Trepp, H Meisterman, T Burgoyne, L Sborgi, AC Cabrera, CY Chen, JC Hoflack, T Kam-Thong, R Schmucki, L Badi, N Flint, ZE Ghiani, F Delobel, C Stucki, G Gromo, A Einhaus, B Hornsperge, S Golling, J Siebourg-P, F Gerber, B Bohrmann, C Futter, T Dunkley, S Hiller, O Schilling, V Enzmann, S Fauser, M Plodinec, R Iacone HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model EBioMedicine, 2017-12-13;0(0):. 2017-12-13 [PMID: 29269042] (ICC, Western Blot, Human)
• R Yentrapall, J Merl-Pham, O Azimzadeh, L Mutschelkn, C Peters, SM Hauck, MJ Atkinson, S Tapio, S Moertl Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation Int. J. Radiat. Biol., 2017-03-07;0(0):1-12. 2017-03-07 [PMID: 28264626] (Western Blot, Human)
• Matsui T, Higashimoto Y, Taira J, Yamagishi S Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells. Biochem Biophys Res Commun, 2013-10-23;441(2):405-10. 2013-10-23 [PMID: 24161393] (Western Blot, Human)
• Shahinian H, Loessner D, Biniossek M, Kizhakkedathu J, Clements J, Magdolen V, Schilling O Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFbeta-1 signaling in ovarian cancer cells. Mol Oncol, 2013-10-01;8(1):68-82. 2013-10-01 [PMID: 24120346] (Western Blot, Human)
• Haurigot V, Villacampa P, Ribera A, Bosch A, Ramos D, Ruberte J, Bosch F Long-Term Retinal PEDF Overexpression Prevents Neovascularization in a Murine Adult Model of Retinopathy. PLoS ONE, 2012-07-20;7(7):e41511. 2012-07-20 [PMID: 22911805] (IHC-P, Western Blot, Mouse)
• Hou HY, Liang HL, Wang YS A therapeutic strategy for choroidal neovascularization based on recruitment of mesenchymal stem cells to the sites of lesions. Mol. Ther., 2010-07-20;18(10):1837-45. 2010-07-20 [PMID: 20647999] (IHC-Fr, Mouse)
• Murakami Y, Ikeda Y, Yonemitsu Y, Onimaru M, Nakagawa K, Kohno R, Miyazaki M, Hisatomi T, Nakamura M, Yabe T, Hasegawa M, Ishibashi T, Sueishi K Inhibition of nuclear translocation of apoptosis-inducing factor is an essential mechanism of the neuroprotective activity of pigment epithelium-derived factor in a rat model of retinal degeneration. Am. J. Pathol., 2008-10-09;173(5):1326-38. 2008-10-09 [PMID: 18845835] (Neutralization, Human)

Bioinformatics

• Gene Symbol: SERPINF1
• Uniprot:
  • P36955()