Serpin F1/PEDF Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human breast tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 56 kDa (as indicated) using 2 µg/mL of ...read more
Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Serpin F1/PEDF was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) at 15 µg/mL ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Serpin F1/PEDF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Serpin F1/PEDF
Asp44-Pro418
Accession # P36955
Specificity
Detects human Serpin F1/PEDF in direct ELISAs. Detects human and mouse Serpin F1/PEDF in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINF1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF1177 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin F1/PEDF Antibody [Unconjugated]

  • Cell proliferation-inducing gene 35 protein
  • EPC-1
  • EPC-1PIG35
  • PEDF
  • PEDFpigment epithelium-derived factor
  • pigment epithelium derived factor), member 1
  • proliferation-inducing protein 35
  • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin
  • Serpin F1
  • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Background

Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human Serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387‑411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44‑418, human Serpin F1 shares 87% aa identity with mouse Serpin F1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin F1/PEDF Antibody (AF1177)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Agonist Activity, IHC-Fr, Neutralization, Western Blot.


Filter By Application
Agonist Activity
(1)
IHC-Fr
(1)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1177 Applications Species
V Vigneswara, Z Ahmed Pigment epithelium-derived factor mediates retinal ganglion cell neuroprotection by suppression of caspase-2 Cell Death Dis, 2019;10(2):102. 2019 [PMID: 30718480] (Agonist Activity, Rat) Agonist Activity Rat
E Melo, P Oertle, C Trepp, H Meisterman, T Burgoyne, L Sborgi, AC Cabrera, CY Chen, JC Hoflack, T Kam-Thong, R Schmucki, L Badi, N Flint, ZE Ghiani, F Delobel, C Stucki, G Gromo, A Einhaus, B Hornsperge, S Golling, J Siebourg-P, F Gerber, B Bohrmann, C Futter, T Dunkley, S Hiller, O Schilling, V Enzmann, S Fauser, M Plodinec, R Iacone HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model EBioMedicine, 2017;0(0):. 2017 [PMID: 29269042] (Western Blot, Human) Western Blot Human
R Yentrapall, J Merl-Pham, O Azimzadeh, L Mutschelkn, C Peters, SM Hauck, MJ Atkinson, S Tapio, S Moertl Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation Int. J. Radiat. Biol., 2017;0(0):1-12. 2017 [PMID: 28264626] (Western Blot, Human) Western Blot Human
Matsui T, Higashimoto Y, Taira J, Yamagishi S Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells. Biochem Biophys Res Commun, 2013;441(2):405-10. 2013 [PMID: 24161393] (Western Blot, Human) Western Blot Human
Shahinian H, Loessner D, Biniossek M, Kizhakkedathu J, Clements J, Magdolen V, Schilling O Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFbeta-1 signaling in ovarian cancer cells. Mol Oncol, 2014;8(1):68-82. 2014 [PMID: 24120346] (Western Blot, Human) Western Blot Human
Haurigot V, Villacampa P, Ribera A, Bosch A, Ramos D, Ruberte J, Bosch F Long-Term Retinal PEDF Overexpression Prevents Neovascularization in a Murine Adult Model of Retinopathy. PLoS ONE, 2012;7(7):e41511. 2012 [PMID: 22911805] (Western Blot, Mouse) Western Blot Mouse
Hou HY, Liang HL, Wang YS A therapeutic strategy for choroidal neovascularization based on recruitment of mesenchymal stem cells to the sites of lesions. Mol. Ther., 2010;18(10):1837-45. 2010 [PMID: 20647999] (IHC-Fr, Mouse) IHC-Fr Mouse
Murakami Y, Ikeda Y, Yonemitsu Y, Onimaru M, Nakagawa K, Kohno R, Miyazaki M, Hisatomi T, Nakamura M, Yabe T, Hasegawa M, Ishibashi T, Sueishi K Inhibition of nuclear translocation of apoptosis-inducing factor is an essential mechanism of the neuroprotective activity of pigment epithelium-derived factor in a rat model of retinal degeneration. Am. J. Pathol., 2008;173(5):1326-38. 2008 [PMID: 18845835] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol SERPINF1
Uniprot