Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human breast tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 56 kDa (as indicated) using 2 µg/mL of ...read more
Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Serpin F1/PEDF was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) at 15 µg/mL ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Serpin F1/PEDF Antibody [Unconjugated]
Cell proliferation-inducing gene 35 protein
PEDFpigment epithelium-derived factor
pigment epithelium derived factor), member 1
proliferation-inducing protein 35
serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1
Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human Serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387‑411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44‑418, human Serpin F1 shares 87% aa identity with mouse Serpin F1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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