Serpin A8/Angiotensinogen Antibody [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 405

Serpin A8/Angiotensinogen Antibody [Alexa Fluor® 405] Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human Angiotensinogen protein (between residues 1-50) [UniProt P01019]
Localization
Secreted (Cytoplasm).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Angiotensinogen antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~53 kDa representing Angiotensinogen. In ICC/IF, cytoplasmic staining was observed in HepG2 cells. In IHC-P, secreted and cytoplasmic staining was observed in mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Serpin A8/Angiotensinogen Antibody [Alexa Fluor® 405]

  • AGT
  • alpha-1 antiproteinase, antitrypsin
  • Angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • Angiotensinogen
  • ANHU
  • Aogen
  • FLJ92595
  • FLJ97926
  • PAT
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP1-30027AF488
Alexa Fluor 647 Labeled NBP1-30027AF647
Alexa Fluor 700 Labeled NBP1-30027AF700

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Bioinformatics Tool for Serpin A8/Angiotensinogen Antibody (NBP1-30027AF405)

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Bioinformatics

Gene Symbol AGT
Entrez