| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30027SS | |||
HAF019). A specific band was detected for Serpin A8/Angiotensinogen at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin A8/Angiotensinogen at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3156-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35581-20ul | |||
HAF016). A specific band was detected for Serpin A8/Angiotensinogen at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6966) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A8/Angiotensinogen at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6966-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3156-SP | |||
HAF005). Specific bands were detected for Serpin A8/Angiotensinogen at approximately 52-62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Serpin A8/Angiotensinogen at approximately 52-62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6966-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89736-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87053-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89737-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98259-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90541-50ul | |||
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP1-30027-img0013.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.")
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30027-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody - BSA Free [NBP1-30027] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Serpin A8 (NBP1-30027) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-30027-img0015.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-30027-img0012.jpg)
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunohistochemistry: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202415111855.jpg "Immunohistochemistry: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunohistochemistry analysis of paraffin-embedded Human placenta using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202414163056.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Western blot analysis of various lysates using Serpin A8/Angiotensinogen Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202414123818.jpg)
![Immunocytochemistry/ Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunofluorescence analysis of HepG2 cells using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202415121397.jpg)
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the brush border in intestinal epithelium. View our protocol for 
CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal epithelial cells. View our protocol for ![Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Western-Blot-NBP2-89736-img0002.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Analysis of Human AGT expression on HepG2 cells. The cells were treated ,stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Flow-Cytometry-NBP2-89736-img0004.jpg)
![Immunoprecipitation: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SerpinA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Immunoprecipitation-NBP2-89736-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89736 antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89736_rabbit-monoclonal-serpin-a8-angiotensinogen-antibody-001-23520251129370.png)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-87053] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-87053-img0006.jpg "Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-87053] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.")
![Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] - Western blot analysis of various lysates using Serpin A8/Angiotensinogen Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-33190_rabbit-serpin-a8-angiotensinogen-mab-9q5d4-1012202411353046.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] - Western blot analysis of various lysates using Serpin A8/Angiotensinogen Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.")
![Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] - Western blot analysis of lysates from Mouse liver, using Serpin A8/Angiotensinogen Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33190_rabbit-serpin-a8-angiotensinogen-mab-9q5d4-1012202411404026.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody (034) [NBP2-89737] - Staining of human AGT in human brain with rabbit monoclonal antibody (1:200). Positive staining was localized to nerve cell.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89737-img0002.jpg "Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody (034) [NBP2-89737] - Staining of human AGT in human brain with rabbit monoclonal antibody (1:200). Positive staining was localized to nerve cell.")
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody (034) [NBP2-89737] - Staining of human AGT in human cirrhosis with rabbit monoclonal antibody (1:200). Positive staining was localized to hepatocyte.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89737-img0001.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP2-98259] - Anti-Serpin A8/Angiotensinogen rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP2-98259-img0001.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody [NBP2-98259] - Anti-Serpin A8/Angiotensinogen rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa (We are unsure as to the identity of these extra bands).")
![Western Blot: Serpin A8/Angiotensinogen Antibody (001) [NBP2-90541] - Anti-Serpin A8/Angiotensinogen rabbit monoclonal antibody at 1:1000 dilution. Lane A: Mouse spleen tissue Lysate. Lane B: Mouse liver tissue Lysate. Lane C: Mouse kidney tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-001-Western-Blot-NBP2-90541-img0001.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (001) [NBP2-90541] - Anti-Serpin A8/Angiotensinogen rabbit monoclonal antibody at 1:1000 dilution. Lane A: Mouse spleen tissue Lysate. Lane B: Mouse liver tissue Lysate. Lane C: Mouse kidney tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa")
