Serpin A12 Antibody

Western Blot: Serpin A12 Antibody [NBP1-20972] - Analysis of SERPINA12 in Human Adipose lysate (35ug protein in RIPA buffer) using NBP1-20972 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Serpin A12 Antibody Summary

• Description: Novus Biologicals Goat Serpin A12 Antibody (NBP1-20972) is a polyclonal antibody validated for use in WB and ELISA. Anti-Serpin A12 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RARWKHEFDPNVTK corresponding to internal region according to NP_776249.1.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINA12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in human adipose lysates (calculated MW of 47.2 kDa band according to NP_776249.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin A12 Antibody

• antitrypsin), member 12
• member 12
• OL-64
• OL-64Visceral adipose-specific serpin
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A12
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• Vaspin
• Visceral adipose tissue-derived serine protease inhibitor

Background

SERPINA12 may modulates insulin action conceivably only in the presence of its yet undefined target proteases in whiteadipose tissues

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A12 Antibody (NBP1-20972)(1)

Publication using NBP1-20972

• Kloting N, Berndt J, Kralisch S et al. Vaspin gene expression in human adipose tissue: association with obesity and type 2 diabetes. Biochem Biophys Res Commun 2006-01-06 [PMID: 16298335]

Bioinformatics

• Gene Symbol: SERPINA12
• Entrez:
  • 145264(Human)
• Uniprot:
  • NP_776249.1(Human)