Intelectin-1/Omentin Antibody [Unconjugated]

Intelectin‑1/Omentin was detected in immersion fixed frozen sections of mouse intestine using 10 µg/mL Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Intelectin‑1/Omentin was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intelectin‑1/Omentin was detected in immersion fixed sections of ileum ALI using Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to mucus granules in goblet cells. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Intelectin-1/Omentin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Intelectin-1/Omentin
  Thr19-Ser298
  Accession # Q8WWA0
• Specificity: Detects human Intelectin-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITLN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-1/Omentin Antibody [Unconjugated]

• Endothelial lectin HL-1
• FLJ20022
• Galactofuranose-binding lectin
• hIntL
• HL-1
• intelectin 1 (galactofuranose binding)
• Intelectin1
• Intelectin-1
• INTLHL1
• ITLN1
• Itlna
• ITLNITLN-1
• LFR
• LFRIntestinal lactoferrin receptor
• Omentin
• Omentin-1

Background

Intelectin-1 (intestinal lectin; also ITLN-1 and omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Intelectin-1/Omentin Antibody (AF4254)(4)

Publications using AF4254

• A Barbe, P Kurowska, E Mlyczy?ska, C Ramé, C Staub, E Venturi, Y Billon, A Rak, J Dupont Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and the onset of puberty Gen. Comp. Endocrinol., 2020-08-19;0(0):113584. 2020-08-19 [PMID: 32827511] (Western Blot, Porcine)
• M Howell, DG Dumitrescu, LR Blankenshi, D Herkert, SK Hatzios Functional characterization of a subtilisin-like serine protease from Vibrio cholerae J. Biol. Chem., 2019-05-10;0(0):. 2019-05-10 [PMID: 31076508] (Western Blot, Human)
• Greulich , Sabrina, Chen , Weena J, Maxhera , Bujar, Rijzewijk , Luuk J, van der Meer , Rutger W, Jonker , Jacqueli, Mueller , Heidi, de Wiza , Daniella, Floerke , Ralf-Rue, Smiris , Konstant, Lamb , Hildo J, de Roos , Albert, Bax , Jeroen J, Romijn , Johannes, Smit , Jan W A, Akhyari , Payam, Lichtenberg , Artur, Eckel , Juergen, Diamant , Michaela, Ouwens , D Margri Cardioprotective properties of omentin-1 in type 2 diabetes: evidence from clinical and in vitro studies. PLoS ONE, 2013-03-29;8(3):e59697. 2013-03-29 [PMID: 23555749] (Western Blot, Human)
• Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation. J. Biol. Chem., 2011-09-12;286(44):38231-41. 2011-09-12 [PMID: 21911487] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ITLN1
• Uniprot:
  • Q8WWA0()