Intelectin-1/Omentin Antibody [Unconjugated]

Intelectin‑1/Omentin was detected in immersion fixed frozen sections of mouse intestine using 10 µg/mL Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Intelectin-1/Omentin was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-Human Intelectin-1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intelectin‑1/Omentin was detected in immersion fixed sections of ileum ALI using Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to mucus granules in goblet cells. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). Points coloured red indicate genes significant at 5% permutation test. Selected highly significant genes are labelled. b Bar chart showing the percentage of cells in the ‘goblet-type’ cell cluster (n = 98) expressing MUC2, ITLN1 or SPINK4 alone or in different combinations (thresholds set at the tenth centile to include 90% of cells in which at least one transcript was detected from each gene). c Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal colon from a resection specimen (blue stain is DAPI). Scale bar, 100 µm. d Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal oesophageal epithelium obtained by endoscopic biopsy (blue stain is DAPI). OSGs encroaching on the surface epithelium are shown in the enlarged images on the right. Scale bars are 200 µm and 50 µm in enlarged images. e Triple immunofluorescence staining images of KRT14 (white), KRT7 (green) and MUC2 (red) in an OSG beneath normal squamous epithelium from an endoscopic biopsy of normal squamous epithelium from a patient with BO biopsy (blue stain is DAPI). Scale bar 50 µm. f Representative immunofluorescence staining of Barrett’s EMR specimen containing intestinal metaplasia but no dysplasia for MUC2 (red), ITLN1 (white) and SPINK4 (green); nuclei (DAPI) in blue. Scale bars are 400 µm and 100 µm in enlarged images Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30323168), licensed under a CC-BY license. Not internally tested by R&D Systems.

SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). Points coloured red indicate genes significant at 5% permutation test. Selected highly significant genes are labelled. b Bar chart showing the percentage of cells in the ‘goblet-type’ cell cluster (n = 98) expressing MUC2, ITLN1 or SPINK4 alone or in different combinations (thresholds set at the tenth centile to include 90% of cells in which at least one transcript was detected from each gene). c Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal colon from a resection specimen (blue stain is DAPI). Scale bar, 100 µm. d Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal oesophageal epithelium obtained by endoscopic biopsy (blue stain is DAPI). OSGs encroaching on the surface epithelium are shown in the enlarged images on the right. Scale bars are 200 µm and 50 µm in enlarged images. e Triple immunofluorescence staining images of KRT14 (white), KRT7 (green) and MUC2 (red) in an OSG beneath normal squamous epithelium from an endoscopic biopsy of normal squamous epithelium from a patient with BO biopsy (blue stain is DAPI). Scale bar 50 µm. f Representative immunofluorescence staining of Barrett’s EMR specimen containing intestinal metaplasia but no dysplasia for MUC2 (red), ITLN1 (white) and SPINK4 (green); nuclei (DAPI) in blue. Scale bars are 400 µm and 100 µm in enlarged images Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30323168), licensed under a CC-BY license. Not internally tested by R&D Systems.

SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). Points coloured red indicate genes significant at 5% permutation test. Selected highly significant genes are labelled. b Bar chart showing the percentage of cells in the ‘goblet-type’ cell cluster (n = 98) expressing MUC2, ITLN1 or SPINK4 alone or in different combinations (thresholds set at the tenth centile to include 90% of cells in which at least one transcript was detected from each gene). c Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal colon from a resection specimen (blue stain is DAPI). Scale bar, 100 µm. d Triple immunofluorescence staining images of MUC2 (red), ITLN1 (white) and SPINK4 (green) in normal oesophageal epithelium obtained by endoscopic biopsy (blue stain is DAPI). OSGs encroaching on the surface epithelium are shown in the enlarged images on the right. Scale bars are 200 µm and 50 µm in enlarged images. e Triple immunofluorescence staining images of KRT14 (white), KRT7 (green) and MUC2 (red) in an OSG beneath normal squamous epithelium from an endoscopic biopsy of normal squamous epithelium from a patient with BO biopsy (blue stain is DAPI). Scale bar 50 µm. f Representative immunofluorescence staining of Barrett’s EMR specimen containing intestinal metaplasia but no dysplasia for MUC2 (red), ITLN1 (white) and SPINK4 (green); nuclei (DAPI) in blue. Scale bars are 400 µm and 100 µm in enlarged images Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30323168), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Intelectin-1/Omentin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Intelectin-1/Omentin
  Thr19-Ser298
  Accession # Q8WWA0
• Specificity: Detects human Intelectin-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITLN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-1/Omentin Antibody [Unconjugated]

• Endothelial lectin HL-1
• FLJ20022
• Galactofuranose-binding lectin
• hIntL
• HL-1
• intelectin 1 (galactofuranose binding)
• Intelectin1
• Intelectin-1
• INTLHL1
• ITLN1
• Itlna
• ITLNITLN-1
• LFR
• LFRIntestinal lactoferrin receptor
• Omentin
• Omentin-1

Background

Intelectin-1 (intestinal lectin; also ITLN-1 and omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Intelectin-1/Omentin Antibody (AF4254)(8)

Publications using AF4254

• Juan D. Matute, Jinzhi Duan, Magdalena B. Flak, Paul Griebel, Jose A. Tascon-Arcila, Shauni Doms et al. Intelectin-1 binds and alters the localization of the mucus barrier-modifying bacterium Akkermansia muciniphila J Exp Med 2023-01-02 [PMID: 36413219]
• A Barbe, P Kurowska, E Mlyczy?ska, C Ramé, C Staub, E Venturi, Y Billon, A Rak, J Dupont Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and the onset of puberty Gen. Comp. Endocrinol., 2020-08-19;0(0):113584. 2020-08-19 [PMID: 32827511] (Western Blot, Porcine)
• M Howell, DG Dumitrescu, LR Blankenshi, D Herkert, SK Hatzios Functional characterization of a subtilisin-like serine protease from Vibrio cholerae J. Biol. Chem., 2019-05-10;0(0):. 2019-05-10 [PMID: 31076508] (Western Blot, Human)
• Richard Peter Owen, Michael Joseph White, David Tyler Severson, Barbara Braden, Adam Bailey, Robert Goldin et al. Single cell RNA-seq reveals profound transcriptional similarity between Barrett’s oesophagus and oesophageal submucosal glands Nature Communications 2018-10-15 [PMID: 30323168]
• Stavroula K. Hatzios, Sören Abel, Julianne Martell, Troy Hubbard, Jumpei Sasabe, Diana Munera et al. Chemoproteomic profiling of host and pathogen enzymes active in cholera Nature Chemical Biology 2016-04-01 [PMID: 26900865]
• Darryl A. Wesener, Kittikhun Wangkanont, Ryan McBride, Xuezheng Song, Matthew B. Kraft, Heather L. Hodges et al. Recognition of microbial glycans by human intelectin-1 Nature Structural & Molecular Biology 2015-08-01 [PMID: 26148048]
• Greulich , Sabrina, Chen , Weena J, Maxhera , Bujar, Rijzewijk , Luuk J, van der Meer , Rutger W, Jonker , Jacqueli, Mueller , Heidi, de Wiza , Daniella, Floerke , Ralf-Rue, Smiris , Konstant, Lamb , Hildo J, de Roos , Albert, Bax , Jeroen J, Romijn , Johannes, Smit , Jan W A, Akhyari , Payam, Lichtenberg , Artur, Eckel , Juergen, Diamant , Michaela, Ouwens , D Margri Cardioprotective properties of omentin-1 in type 2 diabetes: evidence from clinical and in vitro studies. PLoS ONE, 2013-03-29;8(3):e59697. 2013-03-29 [PMID: 23555749] (Western Blot, Human)
• Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation. J. Biol. Chem., 2011-09-12;286(44):38231-41. 2011-09-12 [PMID: 21911487] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ITLN1
• Uniprot:
  • Q8WWA0()