Chemerin Antibody

Recombinant Mouse Chemerin (Catalog # 2325-CM) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human Chem R23 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Chemerin (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Chemerin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2325). The ND50 is typically 3-9 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Chemerin Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Chemerin
  Thr17-Ser156
  Accession # Q9DD06
• Specificity: Detects mouse Chemerin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human Chemerin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RARRES2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 3-9 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product and/or its use is the subject of European Patent 1 405 083 B1, US Patents 7,332,291; 7,419,658 and 7,842,453 as well as foreign equivalents licensed to R&amp;D Systems Inc. The purchase of this product is intended for research purposes only, not including the screening of compounds for the development of therapeutic and/or diagnostic products. Buyers may require a separate license to the patent rights for applications beyond such research purposes. For information on licensing please contact Euroscreen SA rue Adrienne Bolland n°47 B-6041 Gosselies Belgium. Phone: +32-71-348500, Fax: +32-71-348519, e-mail LicensingBD@euroscreen.com. Attention: Dr. Vincent Lannoy.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chemerin Antibody

• Chemerin
• RARRES2
• RAR-responsive protein TIG2
• retinoic acid receptor responder (tazarotene induced) 2
• retinoic acid receptor responder protein 2
• Tazarotene-induced gene 2 protein
• TIG-2
• TIG2HP10433

Background

Mouse Chemerin, also known as Tazarotene-induced Gene-2 (TIG2), is a new, but distant member of the cystatin superfamily (1-3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (2, 3). Chemerin is synthesized as a 162 amino acid (aa) precursor that contains a hydrophobic N-terminal sequence, an intervening 140 aa cystatin-fold containing domain, and a six aa C-terminal prosegment (4-6). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the characteristic fold (4, 7). The precursor molecule is described as undergoing proteolytic processing at both termini by unknown proteases. The N-terminal 16 residue hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (5, 8). In either case it gives rise to a soluble proform that undergoes further processing at the C‑terminus (5). In mouse, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 17-156) (5-7). A shorter form has been described in human (7). The activity seems to be concentrated in the nine aa’s preceding the prosegment (aa 148-156). Retention of the prosegment blocks activity (4). The 140 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (5). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature mouse Chemerin shares 67%, 84%, and 82% aa sequence identity to human, rat, and hamster Chemerin, respectively (6). There is apparently cross-species activity for the protein (6).

1. Nagpal, S. et al. (1997) J. Invest. Dermatol. 109:91.
2. Storici, P. et al. (1996) Eur. J. Biochem. 238:769.
3. Zanetti, M. (2004) J. Leukoc. Biol. 75:39.
4. Wittamer, V. et al. (2004) J. Biol. Chem. 279:9956.
5. Wittamer, V. et al. (2003) J. Exp. Med. 198:977.
6. Busmann, A. et al. (2004) J. Chromatog. B 811:217.
7. Meder, W. et al. (2003) FEBS Lett. 555:495.
8. Yokoyama-Kobayashi, M. et al. (1999) Gene 228:161.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chemerin Antibody (AF2325)(10)

Publications using AF2325

• I Kim, H Park, I Hwang, D Moon, H Yun, EJ Lee, HS Kim Discovery of chemerin as the new chemoattractant of human mesenchymal stem cells Cell & bioscience, 2021;11(1):120. 2021-01-01 [PMID: 34210352] (Western Blot, Mouse)
• E Gronseth, A Gupta, C Koceja, S Kumar, RG Kutty, K Rarick, L Wang, R Ramchandra Astrocytes influence medulloblastoma phenotypes and CD133 surface expression PLoS ONE, 2020;15(7):e0235852. 2020-01-01 [PMID: 32628717] (Western Blot, Zebrafish)
• Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia Nat Commun, 2016;7(0):12528. 2016-01-01 [PMID: 27538380] (Neutralization, Mouse)
• Provoost S, De Grove K, Fraser G, Lannoy V, Tournoy K, Brusselle G, Maes T, Joos G Pro- and Anti-Inflammatory Role of ChemR23 Signaling in Pollutant-Induced Inflammatory Lung Responses. J Immunol, 2016;196(4):1882-90. 2016-01-01 [PMID: 26773141] (IHC-P, Mouse)
• Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel B, Cichy J The expression and regulation of chemerin in the epidermis. PLoS ONE, 2015;10(2):e0117830. 2015-01-01 [PMID: 25659101]
• Muruganandan S, Dranse H, Rourke J, McMullen N, Sinal C Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis. Stem Cells, 2013;31(10):2172-82. 2013-01-01 [PMID: 23766088] (Western Blot, Mouse)
• Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis. J. Biol. Chem., 2011;286(27):23982-95. 2011-01-01 [PMID: 21572083] (Western Blot, Mouse)
• Demoor T, Bracke KR, Dupont LL The Role of ChemR23 in the Induction and Resolution of Cigarette Smoke-Induced Inflammation. J. Immunol., 2011;186(9):5457-67. 2011-01-01 [PMID: 21430224] (IHC-P, Mouse)
• Cash JL, Christian AR, Greaves DR Chemerin peptides promote phagocytosis in a ChemR23- and Syk-dependent manner. J. Immunol., 2010;184(9):5315-24. 2010-01-01 [PMID: 20363975] (Neutralization, Mouse)
• Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model. J. Immunol., 2009;183(10):6489-99. 2009-01-01 [PMID: 19841182] (ICC, Mouse)

Review for Chemerin Antibody (AF2325) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous - WB Mouse 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23766088
• Species: Mouse

Bioinformatics

• Gene Symbol: RARRES2
• Uniprot:
  • Q9DD06()