Serpin A12 Antibody (VP63) - Azide and BSA Free

Western Blot: Serpin A12 Antibody (VP63) [NBP2-80041] - Western blot analysis using anti-Serpin A12 (human), mAb (VP63) at 1:1000 dilution. 1: Human vaspin (His-tagged). 2: Human vaspin (FLAG-tagged). 3: Mouse IL-33 (His-tagged) (negative control).

Immunohistochemistry: Serpin A12 Antibody (VP63) [NBP2-80041] - Immunohistochemical staining of vaspin with anti-Serpin A12 (human), mAb (VP63) in healthy skin (A). Arrow - stronger staining in Stratum granulosum. Negative control for vaspin in healthy skin (B). Blue hematoxylin stained cell nuclei.

Immunohistochemistry: Serpin A12 Antibody (VP63) [NBP2-80041] - Immunohistochemical staining of vaspin with anti-Serpin A12 (human), mAb (VP63) in psoriatic skin lesions (A). Negative control for vaspin in psoriatic skin lesions (B). Blue hematoxylin stained cell nuclei.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: VP63
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Serpin A12 Antibody (VP63) - Azide and BSA Free Summary

• Immunogen: Recombinant human Serpin A12.
• Specificity: Recognizes human Serpin A12. Detects a band of ~48kDa by Western blot.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINA12
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000 - 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100
  • Western Blot 1:2000 - 1:5000
• Application Notes: Western blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um-filtered solution in PBS, pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Serpin A12 Antibody (VP63) - Azide and BSA Free

• antitrypsin), member 12
• member 12
• OL-64
• OL-64Visceral adipose-specific serpin
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A12
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• Vaspin
• Visceral adipose tissue-derived serine protease inhibitor

Background

SERPINA12 may modulates insulin action conceivably only in the presence of its yet undefined target proteases in whiteadipose tissues

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINA12