Serpin A12 Antibody (3B3) - Azide and BSA Free

Western Blot: Serpin A12 Antibody (3B3) [H00145264-M01] - Analysis of SERPINA12 expression in transfected 293T cell line by SERPINA12 monoclonal antibody (M01), clone 3B3.Lane 1: SERPINA12 transfected lysate(47.2 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Serpin A12 Antibody (3B3) [H00145264-M01] - Analysis of SERPINA12 transfected lysate using anti-SERPINA12 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SERPINA12 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: Serpin A12 Antibody (3B3) [H00145264-M01] - Detection limit for recombinant GST tagged SERPINA12 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 3B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Serpin A12 Antibody (3B3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Serpin A12 Antibody (3B3) - Azide and BSA Free (H00145264-M01) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SERPINA12 (AAH40857, 1 a.a. ~ 414 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNPTLGLAIFLAVLLTVKGLLKPSFSPRNYKALSEVQGWKQRMAAKELARQNMDLGFKLLKKLAFYNPGRNIFLSPLSISTAFSMLCLGAQDSTLDEIKQGFNFRKMPEKDLHEGFHYIIHELTQKTQDLKLSIGNTLFIDQRLQPQRKFLEDAKNFYSAETILTNFQNLEMAQKQINDFISQKTHGKINNLIENIDPGTVMLLANYIFFRARWKHEFDPNVTKEEDFFLEKNSSVKVPMMFRSGIYQVGYDDKLSCTILEIPYQKNITAIFILPDEGKLKHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTGTFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVGEAVHKAELKMDERGTEGAAGTGAQTLPMETPLVVKIDKPYLLLIYSEKIPSVLFLGKIVNPIGK
• Localization: Secreted
• Specificity: SERPINA12 - serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINA12
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Serpin A12 Antibody (3B3) - Azide and BSA Free

• antitrypsin), member 12
• member 12
• OL-64
• OL-64Visceral adipose-specific serpin
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A12
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• Vaspin
• Visceral adipose tissue-derived serine protease inhibitor

Background

SERPINA12 is a adipocytokine expressed predominantly in visceral white adipose tissues. It has three beta-sheets, nine alpha-helices, and one central loop, which are distinctive structural features of Serpin family members. The serpins are irreversible (

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINA12
• Entrez:
  • 145264(Human)
• Uniprot:
  • AAH40857(Human)