Semaphorin 6D Antibody (257510)

• Reactivity: Hu
• Applications: WB
• Clone: 257510
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 6D Antibody (257510) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6D
  Ser22-His660
  Accession # Q8NFY4
• Specificity: Detects human Semaphorin 6D in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Semaphorin 3A and recombinant mouse (rm) Semaphorin 3C is observed and no cross‑reactivity with rhSemaphorin 3B, 6A, 6B, 6C, 7A, rmSemaphorin 3E, 3F, or 6D is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEMA6D
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 6D Antibody (257510)

• KIAA1479FLJ11598
• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
• Sema6D
• Semaphorin 6D
• semaphorin-6D

Background

Semaphorin 6D (Sema6D) is a ~130-135 kDa member of the Semaphorin family of axon guidance molecules (1-3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that share ~40% amino acid (aa) identity and exhibit neuropilin-independent binding to specific plexin A receptors (1, 2). Sema6D is expressed in the cardiac tube and within the brain and spinal cord during development (2-5). It shows broad expression postnatally, including neurons, lymphocytes, dendritic cells and osteoclasts (5-8). Brain, kidney, placenta, and cardiac and skeletal muscles show highest mRNA expression (2, 5). The primary human Sema6D isoform (1073 aa) includes a 21 aa signal sequence, a 642 aa extracellular domain (ECD) including Sema and PSI domains, a 21 aa transmembrane sequence and a 390 aa cytoplasmic portion. The ECD of this isoform shares 96%, 96%, 98%, 98% and 97% aa identity with corresponding mouse, rat, bovine, equine and canine sequences, respectively. Alternate splicing creates isoforms of 1054, 1030, 1017, 1011 and 998 that lack sequences between aa 570 and 644 and/or contain a 13 aa insert after aa 549; all variations affect the PSI domain (2, 5). An isoform of 476 aa is truncated after the Sema domain (2). All Sema6D isoforms are present in the brain, but are differentially expressed elsewhere (2, 5). In the developing brain, both Sema6D and Sema6C are co-expressed with Plexin A1 and interact differentially to guide proprioceptive peripheral neurons by repulsion (4). Sema6D is essential for morphogenesis of the cardiac ventricle in the chick, signaling both forward through Plexin A1 and in reverse through Sema6D (3, 9). Sema6D mediates survival and anchorage-independent growth of malignant pleural mesothelioma (10) It is active in immune responses by Sema6D+ T cell stimulation of dendritic cells, and in bone homeostasis by engaging osteoclast Plexin A1 (6-8). In all these settings, Sema6D acts through a complex of Plexin A1 with receptor tyrosine kinases such as VEGF R2 (3, 6-10).

1. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
2. Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
3. Toyofuku, T. et al. (2004) Genes Dev. 18:435.
4. Yoshida, Y. et al. (2006) Neuron 52:775.
5. Taniguchi, M. and T. Shimizu (2004) Biochem. Biophys. Res. Commun. 314:242.
6. O’Connor, B.P. et al. (2008) Proc. Natl. Acad. Sci. USA 105:13015.
7. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
8. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
9. Toyofuku, T. et al. (2004) Nat. Cell Biol. 6:1204.
10. Catalano, A. et al. (2009) Cancer Res. 69:1485.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 6D Antibody (MAB2095)(1)

Publication using MAB2095

• S Demolli, A Doddaballa, K Devraj, K Stark, Y Manavski, A Eckart, CM Zehendner, T Lucas, T Korff, M Hecker, S Massberg, S Liebner, D Kaluza, RA Boon, S Dimmeler Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D Cardiovasc. Res., 2017-05-01;113(6):681-691. 2017-05-01 [PMID: 28453731] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SEMA6D
• Uniprot:
  • Q8NFY4()