Recombinant Mouse Semaphorin 6D Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 6D Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Semaphorin 6D Fc Chimera is coated at 6 μg/mL, Recombinant Mouse Plexin A1 (Catalog # 4309-PA) binds with an apparent K_D < 50 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Semaphorin 6D protein
  

  Mouse Semaphorin 6D (Ser22 - His641) Accession # NP_954710	IEGRMDP	MouseIgG2A (Glu98 - Lys330)
  N-terminus		C-terminus

• Accession #: NP_954710
• N-terminal Sequence: Ser22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sema6d
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 97 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 6D Fc Chimera Protein, CF

• KIAA1479FLJ11598
• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
• Sema6D
• Semaphorin 6D
• semaphorin-6D

Background

Semaphorin 6D (Sema6D) is a 130 ‑ 135 kDa member of the Semaphorin family of axon guidance molecules (1 ‑ 3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that share approximately 40% amino acid (aa) identity and exhibit neuropilin-independent binding to specific plexin A receptors (1, 2). Sema6D is expressed in the cardiac tube and within the brain and spinal cord during development (2 ‑ 5). It shows broad expression postnatally, including neurons, lymphocytes, dendritic cells and osteoclasts (5 ‑ 8). Brain, kidney, placenta, and cardiac and skeletal muscles show highest mRNA expression (2, 5). The primary mouse Sema6D isoform (1073 aa) includes a 21 aa signal sequence, a 642 aa extracellular domain (ECD) including Sema and PSI domains, a 21 aa transmembrane sequence and a 390 aa cytoplasmic portion. The ECD of this isoform shares 96%, 99%, 95%, 96% and 96% aa identity with corresponding human, rat, bovine, equine and canine sequences, respectively. Alternate splicing creates isoforms of 1054, 1030, 1017, 1011 and 998 that lack sequences between aa 570 and 644 and/or contain a 13 aa insert after aa 549; all variations affect the PSI domain (2, 5). All Sema6D isoforms are present in the brain, but are differentially expressed elsewhere (2, 5). In the developing brain, both Sema6D and Sema6C are co‑expressed with Plexin A1 and interact differentially to guide proprioceptive peripheral neurons by repulsion (4). Sema6D is essential for morphogenesis of the cardiac ventricle in the chick, signaling both forward through Plexin A1 and in reverse through Sema6D (3, 9). Sema6D mediates survival and anchorage‑independent growth of malignant pleural mesothelioma (10) It is active in immune responses by Sema6D+ T cell stimulation of dendritic cells, and in bone homeostasis by engaging osteoclast Plexin A1 (6 ‑ 8). In all these settings, Sema6D acts through a complex of Plexin A1 with receptor tyrosine kinases such as VEGF R2 (3, 6 ‑ 10).

1. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
2. Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
3. Toyofuku, T. et al. (2004) Genes Dev. 18:435.
4. Yoshida, Y. et al. (2006) Neuron 52:775.
5. Taniguchi, M. and T. Shimizu (2004) Biochem. Biophys. Res. Commun. 314:242.
6. O’Connor, B.P. et al. (2008) Proc. Natl. Acad. Sci. USA 105:13015.
7. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
8. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
9. Toyofuku, T. et al. (2004) Nat. Cell Biol. 6:1204.
10. Catalano, A. et al. (2009) Cancer Res. 69:1485.

Publications for Semaphorin 6D (7067-S6)(2)

Publications using 7067-S6

• Hirai, T;Naito, Y;Koyama, S;Nakanishi, Y;Masuhiro, K;Izumi, M;Kuge, T;Naito, M;Mizuno, Y;Yamaguchi, Y;Kang, S;Yaga, M;Futami, Y;Nojima, S;Nishide, M;Morita, T;Kato, Y;Tsuda, T;Takemoto, N;Kinugasa-Katayama, Y;Aoshi, T;Villa, JK;Yamashita, K;Enokida, T;Hoshi, Y;Matsuura, K;Tahara, M;Takamatsu, H;Takeda, Y;Inohara, H;Kumanogoh, A; Sema6D forward signaling impairs T cell activation and proliferation in head and neck cancer JCI insight 2024-02-08 [PMID: 38329122] (Stimulation, Mouse)
• Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H Osteoprotection by semaphorin 3A. Nature, 2012-05-03;485(7396):69-74. 2012-05-03 [PMID: 22522930] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Sema6d
• Uniprot:
  • NP_954710()