Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

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RecombinantHuman Semaphorin 3A Fc Chimera binds to Recombinant Rat Neuropilin-1 Fc Chimerain an ELISA Binding Assay. When Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) is coated at 2µg/ml, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Semaphorin 3A Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) is coated at 2 µg/mL, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with an ED50 = 50-300 ng/mL. Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera causes >50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta ‑NGF (Catalog # 256-GF).
Source
Mouse myeloma cell line, NS0-derived human Semaphorin 3A protein
Met-6-His tag Human Semaphorin 3A
Lys26-Val771
(Arg552Ala & Arg555Ala)
Accession # Q14563
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Met & Ser770
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SEMA3A
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
113.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa, 90 kDa and 37 kDa, reducing conditions
Publications
Read Publications using
1250-S3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

  • (semaphorin) 3A
  • coll-1
  • collapsin 1
  • Hsema-I
  • Hsema-III
  • MGC133243
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema III
  • SEMA1
  • Sema3A
  • SEMAD
  • SEMAIII
  • SEMAL
  • Semaphorin 3A
  • semaphorin D
  • Semaphorin III
  • semaphorin L
  • semaphorin-3A
  • SemD

Background

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2‑like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1-3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

  1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
  2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
  3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
  4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
  5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
  6. Luo Y. et al. (1993) Cell 75:217.

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Publications for Semaphorin 3A (1250-S3)(24)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, N/A.

We have publications tested in 2 applications: Bioassay, ELISA Standard.


Filter By Application
Bioassay
(23)
ELISA Standard
(1)
All Applications
Filter By Species
Human
(14)
Mouse
(1)
Rat
(3)
Chicken
(3)
N/A
(2)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using 1250-S3 Applications Species
JR Podojil, MY Chiang, I Ifergan, R Copeland, LN Liu, S Maloveste, S Langermann, D Liebenson, R Balabanov, H Chi, L Chen, DAA Vignali, SD Miller B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex J. Immunol., 2018;0(0):. 2018 [PMID: 29898965] (Bioassay, Human) Bioassay Human
BJ Grindel, JR Martinez, TV Tellman, DA Harrington, H Zafar, L Nakhleh, LW Chung, MC Farach-Car Matrilysin/MMP-7 Cleavage of Perlecan/HSPG2 Complexed with Semaphorin 3A Supports FAK-Mediated Stromal Invasion by Prostate Cancer Cells Sci Rep, 2018;8(1):7262. 2018 [PMID: 29740048] (Bioassay) Bioassay
D de Ridder, S Marino, RT Bishop, N Renema, C Chenu, D Heymann, AI Idris Bidirectional regulation of bone formation by exogenous and osteosarcoma-derived Sema3A Sci Rep, 2018;8(1):6877. 2018 [PMID: 29720701] (Bioassay, Human) Bioassay Human
M Zhang, Q Zhou, Y Luo, T Nguyen, MI Rosenblatt, VH Guaiquil Semaphorin3A induces nerve regeneration in the adult cornea-a switch from its repulsive role in development PLoS ONE, 2018;13(1):e0191962. 2018 [PMID: 29370308] (Bioassay, Mouse) Bioassay Mouse
T Yokoi, T Tanaka, E Matsuzaka, F Tamalu, SI Watanabe, S Nishina, N Azuma Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells Sci Rep, 2017;7(1):16757. 2017 [PMID: 29196712] (Bioassay, Human) Bioassay Human
BK Hong, S You, SA Yoo, D Park, D Hwang, CS Cho, WU Kim MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis Exp. Mol. Med., 2017;49(8):e363. 2017 [PMID: 28775366] (Bioassay, Human) Bioassay Human
B Palodetto, A da Silva S, M Rodrigues, F Adolfo Cor, F Marconi Ro, F Soares Nie, K Priscila V, A Leda Figue, P Melo Campo, P Favaro, S Teresinha SEMA3A partially reverses VEGF effects through binding to neuropilin-1 Stem Cell Res, 2017;22(0):70-78. 2017 [PMID: 28636974] (Bioassay, Human) Bioassay Human
S Gehler, FV Compere, AM Miller Semaphorin 3A Increases FAK Phosphorylation at Focal Adhesions to Modulate MDA-MB-231 Cell Migration and Spreading on Different Substratum Concentrations Int J Breast Cancer, 2017;2017(0):9619734. 2017 [PMID: 28182100] (Bioassay, Human) Bioassay Human
Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, Langlet F, Hobo B, Cagnoni G, Gallet S, Hanchate N, Mazur D, Taniguchi M, Mazzone M, Verhaagen J, Ciofi P, Bouret S, Tamagnone L, Prevot V Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A. PLoS Biol, 2014;12(3):e1001808. 2014 [PMID: 24618750] (Bioassay, Rat) Bioassay Rat
Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Stem Cells Dev, 2014;23(18):2225-36. 2014 [PMID: 24380401] (Bioassay, Human) Bioassay Human
Show All 24 Publications.

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Bioinformatics

Gene Symbol SEMA3A
Uniprot