Recombinant Human Semaphorin 3A Fc Chimera Protein, CF


RecombinantHuman Semaphorin 3A Fc Chimera binds to Recombinant Rat Neuropilin-1 Fc Chimerain an ELISA Binding Assay. When Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) is coated at 2µg/ml, more

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Semaphorin 3A Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) is coated at 2 µg/mL, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with an ED50 = 50-300 ng/mL. Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera causes >50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta ‑NGF (Catalog # 256-GF).
Mouse myeloma cell line, NS0-derived human Semaphorin 3A protein
Met-6-His tag Human Semaphorin 3A
(Arg552Ala & Arg555Ala)
Accession # Q14563
N-terminus C-terminus
Accession #
N-terminal Sequence
Met & Ser770
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
  • Bioactivity2
Theoretical MW
113.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
120 kDa, 90 kDa and 37 kDa, reducing conditions
Read Publications using
1250-S3 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

  • (semaphorin) 3A
  • coll-1
  • collapsin 1
  • Hsema-I
  • Hsema-III
  • MGC133243
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema III
  • SEMA1
  • Sema3A
  • Semaphorin 3A
  • semaphorin D
  • Semaphorin III
  • semaphorin L
  • semaphorin-3A
  • SemD


The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2‑like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1-3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

  1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
  2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
  3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
  4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
  5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
  6. Luo Y. et al. (1993) Cell 75:217.

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Publications for Semaphorin 3A (1250-S3)(32)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, N/A.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), Stimulation.

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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using 1250-S3 Applications Species
S ?en, CJ Lux, R Erber A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement International Journal of Molecular Sciences, 2021;22(15):. 2021 [PMID: 34361063] (Bioassay, Human) Bioassay Human
E Ivakhnitsk, MR Chin, D Siegel, VH Guaiquil Vinaxanthone inhibits Semaphorin3A induced axonal growth cone collapse in embryonic neurons but fails to block its growth promoting effects on adult neurons Scientific Reports, 2021;11(1):13019. 2021 [PMID: 34155284] (Cell Culture, Human) Cell Culture Human
G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 2021;34(4):108644. 2021 [PMID: 33503438] (Bioassay, Mouse) Bioassay Mouse
DMO Higgins, M Caliva, M Schroeder, B Carlson, PS Upadhyayul, BD Milligan, SH Cheshier, IL Weissman, JN Sarkaria, FB Meyer, JR Henley Semaphorin 3A mediated brain tumor stem cell proliferation and invasion in EGFRviii mutant gliomas BMC Cancer, 2020;20(1):1213. 2020 [PMID: 33302912] (Cell Culture, Human) Cell Culture Human
S Nadanaka, S Miyata, B Yaqiang, JI Tamura, O Habuchi, H Kitagawa Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice Biomolecules, 2020;10(11):. 2020 [PMID: 33143303] (Bioassay, Human) Bioassay Human
M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1 Nat Commun, 2019;10(1):3345. 2019 [PMID: 31350404] (Bioassay, Human) Bioassay Human
M Pavez, AC Thompson, HJ Arnott, CB Mitchell, I D'Atri, EK Don, JK Chilton, EK Scott, JY Lin, KM Young, RJ Gasperini, L Foa STIM1 is required for remodelling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones J. Neurosci., 2019;0(0):. 2019 [PMID: 31023836] (Bioassay, Rat) Bioassay Rat
JR Podojil, MY Chiang, I Ifergan, R Copeland, LN Liu, S Maloveste, S Langermann, D Liebenson, R Balabanov, H Chi, L Chen, DAA Vignali, SD Miller B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex J. Immunol., 2018;0(0):. 2018 [PMID: 29898965] (Bioassay, Human) Bioassay Human
BJ Grindel, JR Martinez, TV Tellman, DA Harrington, H Zafar, L Nakhleh, LW Chung, MC Farach-Car Matrilysin/MMP-7 Cleavage of Perlecan/HSPG2 Complexed with Semaphorin 3A Supports FAK-Mediated Stromal Invasion by Prostate Cancer Cells Sci Rep, 2018;8(1):7262. 2018 [PMID: 29740048] (Bioassay) Bioassay
D de Ridder, S Marino, RT Bishop, N Renema, C Chenu, D Heymann, AI Idris Bidirectional regulation of bone formation by exogenous and osteosarcoma-derived Sema3A Sci Rep, 2018;8(1):6877. 2018 [PMID: 29720701] (Bioassay, Human) Bioassay Human
Show All 32 Publications.

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Gene Symbol SEMA3A