Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

RecombinantHuman Semaphorin 3A Fc Chimera binds to Recombinant Rat Neuropilin-1 Fc Chimerain an ELISA Binding Assay. When Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) is coated at 2µg/ml, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with anED50= 50-300 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Semaphorin 3A Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) is coated at 2 µg/mL, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with an ED 50 = 50-300 ng/mL. Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera causes >50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta -NGF (Catalog # 256-GF).
• Source: Mouse myeloma cell line, NS0-derived human Semaphorin 3A protein

  Met-6-His tag

  Human Semaphorin 3A Lys26-Val771 (Arg552Ala & Arg555Ala) Accession # Q14563

  DIEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: Q14563
• N-terminal Sequence: Met & Ser770
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SEMA3A
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 113.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120 kDa, 90 kDa and 37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

• (semaphorin) 3A
• coll-1
• collapsin 1
• Hsema-I
• Hsema-III
• MGC133243
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema III
• SEMA1
• Sema3A
• SEMAD
• SEMAIII
• SEMAL
• Semaphorin 3A
• semaphorin D
• Semaphorin III
• semaphorin L
• semaphorin-3A
• SemD

Background

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2). Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1-3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
6. Luo Y. et al. (1993) Cell 75:217.

Publications for Semaphorin 3A (1250-S3)(35)

Publications using 1250-S3

• Koralewska, N;Corradi, E;Milewski, MC;Masante, L;Szczepanska, A;Kierzek, R;Figlerowicz, M;Baudet, ML;Kurzynska-Kokorniak, A; Short 2'-O-methyl/LNA oligomers as highly-selective inhibitors of miRNA production in vitro and in vivo Nucleic acids research 2024-04-27 [PMID: 38676942] (Bioassay, Frog - Xenopus (African Clawed Frog))
• Jeon, HM;Shin, YJ;Lee, J;Chang, N;Woo, DH;Lee, WJ;Nguyen, D;Kang, W;Cho, HJ;Yang, H;Lee, JK;Sa, JK;Lee, Y;Kim, D;Purow, BW;Yoon, Y;Nam, DH;Lee, J; The Semaphorin 3A-Neuropilin-1 Pathway Promotes Clonogenic Growth of Glioblastoma via Activation of TGF? Signaling JCI insight 2023-10-03 [PMID: 37788099] (Cell Culture, Human)
• S ?en, CJ Lux, R Erber A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement International Journal of Molecular Sciences, 2021-08-02;22(15):. 2021-08-02 [PMID: 34361063] (Bioassay, Human)
• E Ivakhnitsk, MR Chin, D Siegel, VH Guaiquil Vinaxanthone inhibits Semaphorin3A induced axonal growth cone collapse in embryonic neurons but fails to block its growth promoting effects on adult neurons Scientific Reports, 2021-06-21;11(1):13019. 2021-06-21 [PMID: 34155284] (Cell Culture, Human)
• Antonio P. A. Ferreira, Alessandra Casamento, Sara Carrillo Roas, Els F. Halff, James Panambalana, Shaan Subramaniam et al. Cdk5 and GSK3 beta inhibit fast endophilin-mediated endocytosis Nature Communications 2021-04-23 [PMID: 33893293] (Bioassay, Human)
• G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 2021-01-26;34(4):108644. 2021-01-26 [PMID: 33503438] (Bioassay, Mouse, Transgenic Mouse)
• DMO Higgins, M Caliva, M Schroeder, B Carlson, PS Upadhyayul, BD Milligan, SH Cheshier, IL Weissman, JN Sarkaria, FB Meyer, JR Henley Semaphorin 3A mediated brain tumor stem cell proliferation and invasion in EGFRviii mutant gliomas BMC Cancer, 2020-12-10;20(1):1213. 2020-12-10 [PMID: 33302912] (Cell Culture, Human)
• S Nadanaka, S Miyata, B Yaqiang, JI Tamura, O Habuchi, H Kitagawa Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice Biomolecules, 2020-10-30;10(11):. 2020-10-30 [PMID: 33143303] (Bioassay, Human)
• M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1 Nat Commun, 2019-07-26;10(1):3345. 2019-07-26 [PMID: 31350404] (Bioassay, Human)
• M Pavez, AC Thompson, HJ Arnott, CB Mitchell, I D'Atri, EK Don, JK Chilton, EK Scott, JY Lin, KM Young, RJ Gasperini, L Foa STIM1 is required for remodelling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones J. Neurosci., 2019-04-25;0(0):. 2019-04-25 [PMID: 31023836] (Bioassay, Rat)
• JR Podojil, MY Chiang, I Ifergan, R Copeland, LN Liu, S Maloveste, S Langermann, D Liebenson, R Balabanov, H Chi, L Chen, DAA Vignali, SD Miller B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex J. Immunol., 2018-06-13;0(0):. 2018-06-13 [PMID: 29898965] (Bioassay, Human, Mouse)
• BJ Grindel, JR Martinez, TV Tellman, DA Harrington, H Zafar, L Nakhleh, LW Chung, MC Farach-Car Matrilysin/MMP-7 Cleavage of Perlecan/HSPG2 Complexed with Semaphorin 3A Supports FAK-Mediated Stromal Invasion by Prostate Cancer Cells Sci Rep, 2018-05-08;8(1):7262. 2018-05-08 [PMID: 29740048] (Bioassay)
• D de Ridder, S Marino, RT Bishop, N Renema, C Chenu, D Heymann, AI Idris Bidirectional regulation of bone formation by exogenous and osteosarcoma-derived Sema3A Sci Rep, 2018-05-02;8(1):6877. 2018-05-02 [PMID: 29720701] (Bioassay, In Vivo, Human, Mouse)
• MW Tang, B Malvar Fer, SP Newsom, JD van Buul, TRDJ Radstake, DL Baeten, PP Tak, KA Reedquist, S García Class 3 semaphorins modulate the invasive capacity of rheumatoid arthritis fibroblast-like synoviocytes Rheumatology (Oxford), 2018-05-01;0(0):. 2018-05-01 [PMID: 29471421] (Stimulation, Human)
• M Zhang, Q Zhou, Y Luo, T Nguyen, MI Rosenblatt, VH Guaiquil Semaphorin3A induces nerve regeneration in the adult cornea-a switch from its repulsive role in development PLoS ONE, 2018-01-25;13(1):e0191962. 2018-01-25 [PMID: 29370308] (Bioassay, Mouse)
• T Yokoi, T Tanaka, E Matsuzaka, F Tamalu, SI Watanabe, S Nishina, N Azuma Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells Sci Rep, 2017-12-01;7(1):16757. 2017-12-01 [PMID: 29196712] (Bioassay, Human)
• BK Hong, S You, SA Yoo, D Park, D Hwang, CS Cho, WU Kim MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis Exp. Mol. Med., 2017-08-04;49(8):e363. 2017-08-04 [PMID: 28775366] (Bioassay, Human)
• B Palodetto, A da Silva S, M Rodrigues, F Adolfo Cor, F Marconi Ro, F Soares Nie, K Priscila V, A Leda Figue, P Melo Campo, P Favaro, S Teresinha SEMA3A partially reverses VEGF effects through binding to neuropilin-1 Stem Cell Res, 2017-06-03;22(0):70-78. 2017-06-03 [PMID: 28636974] (Bioassay, Human)
• S Gehler, FV Compere, AM Miller Semaphorin 3A Increases FAK Phosphorylation at Focal Adhesions to Modulate MDA-MB-231 Cell Migration and Spreading on Different Substratum Concentrations Int J Breast Cancer, 2017-01-15;2017(0):9619734. 2017-01-15 [PMID: 28182100] (Bioassay, Human)
• Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, Langlet F, Hobo B, Cagnoni G, Gallet S, Hanchate N, Mazur D, Taniguchi M, Mazzone M, Verhaagen J, Ciofi P, Bouret S, Tamagnone L, Prevot V Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A. PLoS Biol, 2014-03-11;12(3):e1001808. 2014-03-11 [PMID: 24618750] (Bioassay, Rat)
• Wada , Naohisa, Maeda , Hidefumi, Hasegawa , Daigaku, Gronthos , Stan, Bartold , Peter Ma, Menicanin , Danijela, Fujii , Shinsuke, Yoshida , Shinichi, Tomokiyo , Atsushi, Monnouchi , Satoshi, Akamine , Akifumi Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Stem Cells Dev, 2014-02-10;23(18):2225-36. 2014-02-10 [PMID: 24380401] (Bioassay, Human)
• van Gils , Janine M, Ramkhelawon , Bhama, Fernandes , Luciana, Stewart , Merran C, Guo , Liang, Seibert , Tara, Menezes , Gustavo, Cara , Denise C, Chow , Camille, Kinane , T Bernar, Fisher , Edward A, Balcells , Mercedes, Alvarez-Leite , Jacqueli, Lacy-Hulbert , Adam, Moore , Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9. 2013-02-21 [PMID: 23430612] (Bioassay, Human)
• Chakraborty G, Kumar S, Mishra R Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model. PLoS ONE, 2012-03-20;7(3):e33633. 2012-03-20 [PMID: 22448259] (Bioassay, Human)
• Kabayama H, Takeuchi M, Taniguchi M Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse. J. Neurosci., 2011-05-18;31(20):7357-64. 2011-05-18 [PMID: 21593320] (Bioassay, Chicken)
• Tran-Van H, Avota E, Bortlein C, Mueller N, Schneider-Schaulies S Measles virus modulates dendritic cell/T-cell communication at the level of plexinA1/neuropilin-1 recruitment and activity. Eur. J. Immunol., 2011-01-01;41(1):151-63. 2011-01-01 [PMID: 21182086] (Bioassay, Western Blot, Human)
• Verburg J, Hollenbeck PJ Mitochondrial membrane potential in axons increases with local nerve growth factor or semaphorin signaling. J. Neurosci., 2008-08-13;28(33):8306-15. 2008-08-13 [PMID: 18701693] (Bioassay, Chicken)
• Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J. Clin. Invest., 2008-06-01;118(6):2062-75. 2008-06-01 [PMID: 18483621] (Bioassay, Human)
• Marko SB, Damon DH VEGF promotes vascular sympathetic innervation. Am. J. Physiol. Heart Circ. Physiol., 2008-04-11;294(6):H2646-52. 2008-04-11 [PMID: 18408130] (Bioassay, Rat)
• Narazaki M, Segarra M, Tosato G Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 2008-02-13;111(8):4126-36. 2008-02-13 [PMID: 18272814] (Bioassay, Flow Cytometry, Human)
• Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells. Biochem. Biophys. Res. Commun., 2008-02-07;368(3):543-9. 2008-02-07 [PMID: 18261985] (Bioassay, Human)
• Acevedo LM, Barillas S, Weis SM, Gothert JR, Cheresh DA Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor. Blood, 2008-01-07;111(5):2674-80. 2008-01-07 [PMID: 18180379] (Bioassay, In Vivo, Human, Mouse)
• Jaalouk DE, Ozawa MG, Sun J, Lahdenranta J, Schlingemann RO, Pasqualini R, Arap W The original Pathologische Anatomie Leiden-Endothelium monoclonal antibody recognizes a vascular endothelial growth factor binding site within neuropilin-1. Cancer Res., 2007-10-15;67(20):9623-9. 2007-10-15 [PMID: 17942890] (Bioassay)
• Gallo G RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction. J. Cell. Sci., 2006-08-15;119(0):3413-23. 2006-08-15 [PMID: 16899819] (Bioassay, Chicken)
• Wanigasekara Y, Keast JR Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons. Neuroscience, 2006-07-28;142(2):369-79. 2006-07-28 [PMID: 16876331] (Bioassay, Rat)
• Narazaki M, Tosato G Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A. Blood, 2006-01-19;107(10):3892-901. 2006-01-19 [PMID: 16424390] (ELISA (Standard))

Bioinformatics

• Gene Symbol: SEMA3A
• Uniprot:
  • Q14563()