Semaphorin 6A Antibody

Images

 
Western Blot: Semaphorin 6A Antibody [NBP1-31551] - Sample(30 ug whole cell lysate) A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Semaphorin 6A Antibody [NBP1-31551] - [N2C1], Internal detects SEMA6A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at ...read more
Immunohistochemistry-Paraffin: Semaphorin 6A Antibody [NBP1-31551] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.
Western Blot: Semaphorin 6A Antibody [NBP1-31551] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Simple Western: Semaphorin 6A Antibody [NBP1-31551] - Simple Western lane view shows a specific band for Semaphorin 6A in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Semaphorin 6A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SEMA6A. The exact sequence is proprietary.
Localization
Membrane, Single-pass type I membrane protein
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEMA6A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Simple Western 10 - 15 uL
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Raji Lysate (NB820-59466)
Brain Lysate (NB820-59657)
HepG2 Lysate (NBP1-42569)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Semaphorin 6A Antibody

  • KIAA1368HT018
  • sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
  • Sema VIA
  • SEMA
  • Sema6A
  • SEMA6A1
  • SEMA6A-1
  • Semaphorin 6A
  • semaphorin 6A-1
  • Semaphorin VIA
  • semaphorin-6A
  • semaphorin-6A-1
  • SEMAQ
  • VIA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Semaphorin 6A Antibody (NBP1-31551) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEMA6A
Entrez
Uniprot