Semaphorin 6A Antibody

Western Blot: Semaphorin 6A Antibody [NBP1-31551] - Sample(30 ug whole cell lysate) A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Semaphorin 6A Antibody [NBP1-31551] - [N2C1], Internal detects SEMA6A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEMA6A protein stained by SEMA6A antibody [N2C1], Diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Semaphorin 6A Antibody [NBP1-31551] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.

Western Blot: Semaphorin 6A Antibody [NBP1-31551] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Simple Western: Semaphorin 6A Antibody [NBP1-31551] - Simple Western lane view shows a specific band for Semaphorin 6A in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

Semaphorin 6A Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SEMA6A. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type I membrane protein
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA6A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western 10 - 15 uL
  • Western Blot 1:500-1:3000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Raji Whole Cell Lysate
  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  HepG2 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Semaphorin 6A Antibody

• KIAA1368HT018
• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
• Sema VIA
• SEMA
• Sema6A
• SEMA6A1
• SEMA6A-1
• Semaphorin 6A
• semaphorin 6A-1
• Semaphorin VIA
• semaphorin-6A
• semaphorin-6A-1
• SEMAQ
• VIA

Background

Can act as repulsive axon guidance cues. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SEMA6A
• Entrez:
  • 57556(Human)
  • 20358(Mouse)
• Uniprot:
  • Q9H2E6()