Plexin A2 Antibody [Unconjugated]

Western blot shows lysates of bEnd.3 mouse endothelioma cell line and rat embryonic cortical neuron cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A2 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Plexin A2 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Plexin A2 at approximately 246 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the66-440 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Plexin A2 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2
  Met35-Pro1237
  Accession # P70207
• Specificity: Detects mouse and rat Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A3, and recombinant human Plexin B1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLXNA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 10 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin A2 Antibody [Unconjugated]

• FLJ11751
• FLJ30634
• KIAA0463plexin 2
• OCT
• OCTSemaphorin receptor OCT
• Plexin A2
• PLXN2
• PLXN2plexin-A2
• PLXNA2
• transmembrane protein OCT

Background

Plexin A2 (also OCT) is a 220-230 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, and cardiac neural crest cells. It participates in cell migration and axon guidance, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilin-1 for Sema3A and C. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). There is one alternate start site 10 aa upstream of the standard start site. Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin A2 Antibody (AF5486)(5)

Publications using AF5486

• Barnkob, MB;Michaels, YS;André, V;Macklin, PS;Gileadi, U;Valvo, S;Rei, M;Kulicke, C;Chen, JL;Jain, V;Woodcock, VK;Colin-York, H;Hadjinicolaou, AV;Kong, Y;Mayya, V;Mazet, JM;Mead, GJ;Bull, JA;Rijal, P;Pugh, CW;Townsend, AR;Gérard, A;Olsen, LR;Fritzsche, M;Fulga, TA;Dustin, ML;Jones, EY;Cerundolo, V; Semmaphorin 3?A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells Nature communications 2024-04-12 [PMID: 38609390] (Western Blot, Mouse)
• Lettieri, A;Oleari, R;van den Munkhof, MH;van Battum, EY;Verhagen, MG;Tacconi, C;Spreafico, M;Paganoni, AJJ;Azzarelli, R;Andre', V;Amoruso, F;Palazzolo, L;Eberini, I;Dunkel, L;Howard, SR;Fantin, A;Pasterkamp, RJ;Cariboni, A; SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability Nature communications 2023-12-07 [PMID: 38062045] (Immunohistochemistry, Immunocytochemistry, Mouse, Transgenic Mouse)
• J Szczurkows, A Guo, J Martin, SI Lee, E Martinez, CT Chien, TA Khan, R Singh, D Dadson, TS Tran, S Pautot, M Shelly Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development Cell Reports, 2022-03-15;38(11):110483. 2022-03-15 [PMID: 35294878] (ICC, IHC, Mouse, Rat)
• Rainer H Straub Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis J. Med. Microbiol., 2016-11-14;0(0):. 2016-11-14 [PMID: 27856318] (Neutralization, Mouse)
• Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest, 2015-06-08;125(7):2661-76. 2015-06-08 [PMID: 26053665] (IHC, Mouse)

Bioinformatics

• Gene Symbol: PLXNA2
• Uniprot:
  • P70207()