Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 40-120 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Semaphorin 3F protein
Mouse Semaphorin 3F
Ala19-Pro775 (Arg583Ala and Arg586Ala)
Accession # O88632
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Sema3f
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
111.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-122 kDa, reducing conditions
Publications
Read Publications using
3237-S3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF

  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F
  • sema III/F
  • sema IV
  • Sema3F
  • SEMA4
  • SEMA-IV
  • SEMAK
  • Semaphorin 3F
  • semaphorin III/F
  • semaphorin IV

Background

Semaphorin 3F (Sema 3F; previously Sema IV) is one of six Class 3 (secreted) semaphorins which in the mouse share 40 - 50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema 3F is expressed in the developing nervous system, especially in the dorsal spinal cord (2, 3). In adults, Sema 3F is expressed in the lung and most other tissues (2). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade beta -propeller similar to that found in integrin molecules. Fourteen conserved cysteine residues and one or more N-glycosylation sites are thought to be critical for forming the secondary structure (4). Isoform A is missing aa 153 - 183 within the Sema domain relative to the long form (isoform B) but appears to have similar activity. C-terminal to the Sema domain, Sema 3F has a basic domain, a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and another basic domain at the C-terminus. Dimerization and cleavage at the C-terminus are required for repulsing activity of class 3 semaphorins (5). Mouse Sema 3F shares 96%, 99%, 92%, 97% and 82% aa identity with human, rat, bovine, canine and chick Sema 3F, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema 3F signaling is transduced by type-A plexins, especially Plexin-A3, via interaction with neuropilin-2 (3, 6). Genetic disruption of either Sema 3F or neuropilin-2 alters motor axon trajectory to the ventral forelimb (3). Sema 3F is deleted or downregulated in many metastatic tumors. Restoration of Sema 3F decreases tumorigenicity, vascularization and adhesiveness, most likely through repulsive interactions, VEGF antagonism and downstream integrin regulation (7).

  1. Kruger, R.P. et al. (2005) Nature Rev. Mol. Cell Biol. 6:789.
  2. Eckhardt, F. and A. Meyerhans (1998) Neuroreport 9:3975.
  3. Huber, A.B et al. (2005) Neuron 48:949.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Adams, R.H. et al. (1997) EMBO J. 16:6077.
  6. Yaron, A. et al. (2005) Neuron 45:513.
  7. Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.

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Publications for Semaphorin 3F (3237-S3)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Xenopus.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(5)
In Vivo
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Xenopus
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 3237-S3 Applications Species
F Bajanca, N Gouignard, C Colle, M Parsons, R Mayor, E Theveneau In vivo topology converts competition for cell-matrix adhesion into directional migration Nat Commun, 2019;10(1):1518. 2019 [PMID: 30944331] (Bioassay, Xenopus) Bioassay Xenopus
T Reuer, AC Schneider, B Cakir, AD Bühler, JM Walz, T Lapp, C Lange, H Agostini, G Schlunck, C Cursiefen, T Reinhard, F Bock, A Stahl Semaphorin 3F Modulates Corneal Lymphangiogenesis and Promotes Corneal Graft Survival Invest. Ophthalmol. Vis. Sci., 2018;59(12):5277-5284. 2018 [PMID: 30383199] (In Vivo, Mouse) In Vivo Mouse
Rainer H Straub Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis J. Med. Microbiol., 2016;0(0):. 2016 [PMID: 27856318] (Bioassay, Mouse) Bioassay Mouse
Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci, 2014;34(34):11274-87. 2014 [PMID: 25143608] (Bioassay, Mouse) Bioassay Mouse
Mendes-Da-Cruz D, Brignier A, Asnafi V, Baleydier F, Messias C, Lepelletier Y, Bedjaoui N, Renand A, Smaniotto S, Canioni D, Milpied P, Balabanian K, Bousso P, Lepretre S, Bertrand Y, Dombret H, Ifrah N, Dardenne M, Macintyre E, Savino W, Hermine O Semaphorin 3F and neuropilin-2 control the migration of human T-cell precursors. PLoS ONE, 2014;9(7):e103405. 2014 [PMID: 25068647] (Bioassay, Human) Bioassay Human
Naska S, Lin DC, Miller FD p75NTR is an obligate signaling receptor required for cues that cause sympathetic neuron growth cone collapse. Mol. Cell. Neurosci., 2010;45(2):108-20. 2010 [PMID: 20584617] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Sema3f
Uniprot