Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF


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Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 40-120 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Semaphorin 3F protein
Mouse Semaphorin 3F
Ala19-Pro775 (Arg583Ala and Arg586Ala)
Accession # O88632
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
111.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
110-122 kDa, reducing conditions
Read Publications using
3237-S3 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF

  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F
  • sema III/F
  • sema IV
  • Sema3F
  • SEMA4
  • Semaphorin 3F
  • semaphorin III/F
  • semaphorin IV


Semaphorin 3F (Sema 3F; previously Sema IV) is one of six Class 3 (secreted) semaphorins which in the mouse share 40 - 50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema 3F is expressed in the developing nervous system, especially in the dorsal spinal cord (2, 3). In adults, Sema 3F is expressed in the lung and most other tissues (2). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade beta -propeller similar to that found in integrin molecules. Fourteen conserved cysteine residues and one or more N-glycosylation sites are thought to be critical for forming the secondary structure (4). Isoform A is missing aa 153 - 183 within the Sema domain relative to the long form (isoform B) but appears to have similar activity. C-terminal to the Sema domain, Sema 3F has a basic domain, a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and another basic domain at the C-terminus. Dimerization and cleavage at the C-terminus are required for repulsing activity of class 3 semaphorins (5). Mouse Sema 3F shares 96%, 99%, 92%, 97% and 82% aa identity with human, rat, bovine, canine and chick Sema 3F, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema 3F signaling is transduced by type-A plexins, especially Plexin-A3, via interaction with neuropilin-2 (3, 6). Genetic disruption of either Sema 3F or neuropilin-2 alters motor axon trajectory to the ventral forelimb (3). Sema 3F is deleted or downregulated in many metastatic tumors. Restoration of Sema 3F decreases tumorigenicity, vascularization and adhesiveness, most likely through repulsive interactions, VEGF antagonism and downstream integrin regulation (7).

  1. Kruger, R.P. et al. (2005) Nature Rev. Mol. Cell Biol. 6:789.
  2. Eckhardt, F. and A. Meyerhans (1998) Neuroreport 9:3975.
  3. Huber, A.B et al. (2005) Neuron 48:949.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Adams, R.H. et al. (1997) EMBO J. 16:6077.
  6. Yaron, A. et al. (2005) Neuron 45:513.
  7. Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.

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Publications for Semaphorin 3F (3237-S3)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Xenopus.

We have publications tested in 2 applications: Bioassay, In Vivo.

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Showing Publications 1 - 7 of 7.
Publications using 3237-S3 Applications Species
V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B J. Neurosci., 2019;0(0):. 2019 [PMID: 31182634] (Bioassay, Mouse) Bioassay Mouse
F Bajanca, N Gouignard, C Colle, M Parsons, R Mayor, E Theveneau In vivo topology converts competition for cell-matrix adhesion into directional migration Nat Commun, 2019;10(1):1518. 2019 [PMID: 30944331] (Bioassay, Xenopus) Bioassay Xenopus
T Reuer, AC Schneider, B Cakir, AD Bühler, JM Walz, T Lapp, C Lange, H Agostini, G Schlunck, C Cursiefen, T Reinhard, F Bock, A Stahl Semaphorin 3F Modulates Corneal Lymphangiogenesis and Promotes Corneal Graft Survival Invest. Ophthalmol. Vis. Sci., 2018;59(12):5277-5284. 2018 [PMID: 30383199] (In Vivo, Mouse) In Vivo Mouse
Rainer H Straub Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis J. Med. Microbiol., 2016;0(0):. 2016 [PMID: 27856318] (Bioassay, Mouse) Bioassay Mouse
Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci, 2014;34(34):11274-87. 2014 [PMID: 25143608] (Bioassay, Mouse) Bioassay Mouse
Mendes-Da-Cruz D, Brignier A, Asnafi V, Baleydier F, Messias C, Lepelletier Y, Bedjaoui N, Renand A, Smaniotto S, Canioni D, Milpied P, Balabanian K, Bousso P, Lepretre S, Bertrand Y, Dombret H, Ifrah N, Dardenne M, Macintyre E, Savino W, Hermine O Semaphorin 3F and neuropilin-2 control the migration of human T-cell precursors. PLoS ONE, 2014;9(7):e103405. 2014 [PMID: 25068647] (Bioassay, Human) Bioassay Human
Naska S, Lin DC, Miller FD p75NTR is an obligate signaling receptor required for cues that cause sympathetic neuron growth cone collapse. Mol. Cell. Neurosci., 2010;45(2):108-20. 2010 [PMID: 20584617] (Bioassay, Mouse) Bioassay Mouse

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Gene Symbol Sema3f