Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6B Leu26-Ser603 Accession # Q9H3T3
Detects human and mouse Semaphorin 6B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Semaphorin 6C is observed, 5% cross-reactivity with rhSemaphorin 6D is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, rhSemaphorin 6A, rhSemaphorin 7A, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, and rmSemaphorin 3F is observed.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Semaphorin 6B Antibody
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B
Semaphorin 6B (Sema6B) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1‑4). The four known Class 6 semaphorins are type I transmembrane glycoproteins with Sema domains but without other domains, making them most like Class 1 invertebrate semaphorins in structure (1‑4). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53‑64% within the Sema domain. Sema6B is expressed developmentally in subregions of the nervous system and muscle, and at low levels in most adult tissues (3, 4). Human Sema6B cDNA encodes a 25 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 20 aa transmembrane sequence and a 263 aa cytoplasmic portion. A cytoplasmic proline-rich sequence interacts with the SH3 domain of the c-src signaling protein (4). Full-length Sema6B is thought to form disulfide-linked homodimers (4). Alternate exon splicing creates a 492 aa (presumably) secreted form (Sema6B.1), and a 657 aa form with a shortened cytoplasmic tail (Sema6B.2) (3). Human Sema6B ECD shows 94%, 94%, 96% and 89% aa identity with corresponding mouse, rat, bovine and canine sequences, respectively. Crystal structures of semaphorins reveal that the 500 aa Sema domain forms an integrin-like seven-blade beta -propeller structure stabilized by 14 conserved cysteine residues (5). Sema6B is highly expressed in some glioblastoma and breast cancer cell lines. All-trans retinoic acid slows cancer cell growth and down‑regulates Sema6B expression, probably via dimerization with peroxisome proliferator-activated receptors (PPAR) that have a response element on the Sema6B gene (3, 6, 7). Semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors. Plexin-A4 binds Sema6A (high affinity) and 6B (low affinity) and mediates sympathetic ganglion axon-repulsion, independent of neuropilin-1 (8).
Neufeld, G. et al. (2005) Front. Biosci. 10:751.
Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.
Correa, R. G. et al. (2001) Genomics 73:343.
Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
Collet, P. et al. (2004) Genomics 83:1141.
Murad, H. et al. (2006) Int. J. Oncol. 28:977.
Suto, F. et al. (2005) J. Neurosci. 25:3628.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Semaphorin 6B Antibody and receive a gift card or discount.