Semaphorin 6B Antibody

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Semaphorin 6B Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6B
Leu26-Ser603
Accession # Q9H3T3
Specificity
Detects human and mouse Semaphorin 6B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Semaphorin 6C is observed, 5% cross-reactivity with rhSemaphorin 6D is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, rhSemaphorin 6A, rhSemaphorin 7A, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, and rmSemaphorin 3F is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SEMA6B
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publication using
AF2094 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 6B Antibody

  • sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B
  • Sema6B
  • SEMAN
  • Semaphorin 6B
  • semaphorin VIB
  • semaphorin Z
  • semaphorin-6B
  • semaphorin-6Ba
  • SEMA-VIB
  • semaZ
  • SEM-SEMA-Y
  • UNQ1907/PRO4353

Background

Semaphorin 6B (Sema6B) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1‑4). The four known Class 6 semaphorins are type I transmembrane glycoproteins with Sema domains but without other domains, making them most like Class 1 invertebrate semaphorins in structure (1‑4). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53‑64% within the Sema domain. Sema6B is expressed developmentally in subregions of the nervous system and muscle, and at low levels in most adult tissues (3, 4). Human Sema6B cDNA encodes a 25 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 20 aa transmembrane sequence and a 263 aa cytoplasmic portion. A cytoplasmic proline-rich sequence interacts with the SH3 domain of the c-src signaling protein (4). Full-length Sema6B is thought to form disulfide-linked homodimers (4). Alternate exon splicing creates a 492 aa (presumably) secreted form (Sema6B.1), and a 657 aa form with a shortened cytoplasmic tail (Sema6B.2) (3). Human Sema6B ECD shows 94%, 94%, 96% and 89% aa identity with corresponding mouse, rat, bovine and canine sequences, respectively. Crystal structures of semaphorins reveal that the 500 aa Sema domain forms an integrin-like seven-blade beta -propeller structure stabilized by 14 conserved cysteine residues (5). Sema6B is highly expressed in some glioblastoma and breast cancer cell lines. All-trans retinoic acid slows cancer cell growth and down‑regulates Sema6B expression, probably via dimerization with peroxisome proliferator-activated receptors (PPAR) that have a response element on the Sema6B gene (3, 6, 7). Semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors. Plexin-A4 binds Sema6A (high affinity) and 6B (low affinity) and mediates sympathetic ganglion axon-repulsion, independent of neuropilin-1 (8).

  1. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  2. Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.
  3. Correa, R. G. et al. (2001) Genomics 73:343.
  4. Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
  5. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  6. Collet, P. et al. (2004) Genomics 83:1141.
  7. Murad, H. et al. (2006) Int. J. Oncol. 28:977.
  8. Suto, F. et al. (2005) J. Neurosci. 25:3628.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Semaphorin 6B Antibody (AF2094)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Biotin BAF2094

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Bioinformatics

Gene Symbol SEMA6B
Uniprot