SARM1 Antibody

Images

 
Western Blot: SARM1 Antibody [NBP1-77200] - Analysis of SARM in Daudi cell lysate with SARM antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: SARM1 Antibody [NBP1-77200] - Analysis of SARM in formalin-fixed Daudi cells using NBP1-77200 at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: SARM1 Antibody [NBP1-77200] - Daudi cells at 2ug/ml

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SARM1 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human SARM. The immunogen is located within amino acids 640 - 690 of SARM.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SARM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
SARM antibody can be used for detection of SARM by Western blot at 0.5 to 2 mg/ml.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SARM1 Peptide (NBP1-77200PEP)

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Pig: (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SARM1 Antibody

  • KIAA0524SAM domain-containing protein 2
  • SAMD2
  • SAMD2sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila
  • SARM1
  • SARMFLJ36296
  • Sterile alpha and Armadillo repeat protein
  • sterile alpha and TIR motif containing 1
  • sterile alpha and TIR motif-containing protein 1
  • Sterile alpha motif domain-containing protein 2
  • Tir-1 homolog

Background

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SARM (SAM and ARM-containing protein), along with other molecules such as TIRP, TRIF, TIRAP, and MyD88, is thought to serve as an adaptor protein for the TLRs that allows for the activation of downstream kinases and NF-kappaB, and ultimately the expression of proteins involved in host defense. While SARM has not been conclusively shown to associate directly with TLRs, the presence of a Toll-interluekin-1 (TIR) domain in SARM is consistent with a role as a signaling molecule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SARM1
Entrez
Uniprot