SAM68 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SAM68 Antibody [NBP2-38805] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-38805] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-38805] - Staining of human endometrium shows high expression.

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-38805] - Staining in human endometrium and skeletal muscle tissues using anti-KHDRBS1 antibody. Corresponding KHDRBS1 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SAM68 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SAM68 Antibody - BSA Free (NBP2-38805) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ALVRGTPVRGAITRGATVTRGVPPPPTVRGAPAPRARTAGIQRIPLP
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KHDRBS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SAM68 Protein (NBP2-38805PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SAM68 Antibody - BSA Free

• FLJ34027
• GAP-associated tyrosine phosphoprotein p62
• KH domain containing, RNA binding, signal transduction associated 1
• KH domain-containing, RNA-binding, signal transduction-associated protein 1
• KHDRBS1
• p21 Ras GTPase-activating protein-associated p62
• p62
• p68
• SAM68
• Sam68GAP-associated tyrosine phosphoprotein p62 (Sam68)
• Src-associated in mitosis 68 kDa protein

Background

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export Function: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KHDRBS1
• Uniprot:
  • Q07666()