SALL4 Antibody

Western Blot: SALL4 Antibody [NB100-60559] - Western blot analysis of SALL4 using NB100-60559 at 0.1ug/ml in rat testis lysate (35ug protein in RIPA buffer).

Western Blot: SALL4 Antibody [NB100-60559] - (0.1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SALL4 Antibody Summary

• Description: Novus Biologicals Goat SALL4 Antibody (NB100-60559) is a polyclonal antibody validated for use in WB and ELISA. Anti-SALL4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DQSKKGKGKPQN corresponding to internal region according to Uniprot Mouse NP_780512.2, NP_958797.2, NP_958798.2.
• Epitope: DQSKKGKGKPQN
• Specificity: This antibody is expected to recognize isoform a (NP_780512.2) and isoform b (NP_958797.2).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SALL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in rat spleen and testis lysates (calculated MW of 66.2 kDa band according to NP_958797.2). Additional bands of unknown identity was also consistently observed at 28+24 kDa. These bands were successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SALL4 Antibody

• dJ1112F19.1
• DRRS
• HSAL4
• MGC133050
• SALL4
• sal-like 4 (Drosophila)
• sal-like protein 4
• Zinc finger protein 797
• Zinc finger protein SALL4
• ZNF797
• ZNF797sal (Drosophila)-like 4

Background

Sall4 is a probable transcription factor. Defects in Sall4 are the cause of Okihiro syndrome; also known as Duane radial ray syndrome (DRRS). It is a disorder characterized by the association of forearm malformations with Duane retraction syndrome. Sall4 is also involved in forelimb and heart development in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SALL4 Antibody (NB100-60559)(1)

Publication using NB100-60559

• Wang J, Rao S, Chu J et al. A protein interaction network for pluripotency of embryonic stem cells. Nature 2006-11-16 [PMID: 17093407]

Bioinformatics

• Gene Symbol: SALL4
• Entrez:
  • 99377(Mouse)
• Uniprot:
  • NP_780512.2(Mouse)