SALL4 Antibody (651839)

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.025 µg/mL of Mouse Anti-Human/Mouse SALL4 Monoclonal Antibody (Catalog # MAB6374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SALL4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 651839
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SALL4 Antibody (651839) Summary

• Immunogen: E. coli-derived recombinant human SALL4
  Lys96-Gly359
  Accession # Q9UJQ4
• Specificity: Detects endogenous human and mouse SALL4 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SALL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.025 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SALL4 Antibody (651839)

• dJ1112F19.1
• DRRS
• HSAL4
• MGC133050
• SALL4
• sal-like 4 (Drosophila)
• sal-like protein 4
• Zinc finger protein 797
• Zinc finger protein SALL4
• ZNF797
• ZNF797sal (Drosophila)-like 4

Background

SALL4 (SAL-like protein 4) is a 1324 amino acid (aa) member of the SAL protein family that possess multiple C₂H₂-type zinc-fingers. It is a transcriptional repressor when associated with histone deacetylase and a transcriptional activator of the Wnt pathway in its native form. It contributes to generation of induced pluripotent stem cells and to axis formation during development. It is frequently overexpressed in germ cell tumors and acute myeloid leukemias. Mutations of human SALL4 can result in conditions such as Okihiro, Duane Radial Ray, or Holt-Oram syndromes. The region used as an immunogen is common to both SALL4 and the ubiquitous 617 aa isoform, SALL4B; it shares 76% aa identity between human and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SALL4
• Uniprot:
  • Q9UJQ4(Human)