S100B Antibody (3B10)

Product Discontinued

S100B Antibody (3B10) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human brain S100 proteins.
• Localization: Cytoplasmic
• Specificity: Specific to S100BB.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Immunoassay
  • Sandwich ELISA
  • Western Blot
• Application Notes: Recommended pairs for sandwich immunoassay (coating - conjugate): NB200-406 (Coating) - NB200-405 (Conjugate). Better performance can be obtained in presence of 5 mM CaCl2 in the assay buffers. This antibody is sensitive to EDTA or other bivalent-binding agents.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for S100B Antibody (3B10)

• beta (neural)
• NEF
• S100 beta
• S100 calcium binding protein B
• S100 calcium-binding protein B
• S100 calcium-binding protein, beta (neural)
• S-100 calcium-binding protein, beta chain, 10protein S100-B
• S-100 protein beta chain
• S-100 protein subunit beta
• S100
• S100B
• S100beta

Background

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumors of the skin, and in Langerhans cells in the skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100B
• Uniprot:
  • P23297()