S100B Antibody (15F9NB)

Images

 
Western Blot: S100B Antibody (15F9NB) [NBP2-45267] - Total protein from human brain (1) and mouse brain (2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. ...read more
Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in human prostate tissue.
Flow Cytometry: S100B Antibody (15F9NB) [NBP2-45267] - An intracellular stain was performed on SK-MEL-28 cells with S100B [15F9NB] Antibody NBP2-45267PE (blue) and a matched isotype control (orange). Cells were fixed ...read more
Western Blot: S100B Antibody (15F9NB) [NBP2-45267] - Total protein from human brain (1) and mouse brain (2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells ...read more
Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis of S100B (15F9NB) in human brain.
Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis of S100B in human prostate.
Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in human brain tissue.
Flow Cytometry: S100B Antibody (15F9NB) [NBP2-45267] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (Orange) stained at a dilution of 1:500. Detected with a GtxRb DyLight 488 secondary. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Pm, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
15F9NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

S100B Antibody (15F9NB) Summary

Immunogen
Partial recombinant human S100B protein (amino acids 2-92) [Uniprot: P04271]
Predicted Species
Mouse (99%), Rat (98%), Porcine (97%), Bovine (97%), Rabbit (100%), Sheep (99%), Primate (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1:500
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
Control
S100B Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S100B Antibody (15F9NB)

  • beta (neural)
  • NEF
  • S100 beta
  • S100 calcium binding protein B
  • S100 calcium-binding protein B
  • S100 calcium-binding protein, beta (neural)
  • S-100 calcium-binding protein, beta chain, 10protein S100-B
  • S-100 protein beta chain
  • S-100 protein subunit beta
  • S100
  • S100B
  • S100beta

Background

S100B belongs to the S100 family, which containing 2 EF-hand calcium-binding motifs. In humans, S100B protein is encoded by the S100P gene located at 4q16, but genes that encode other numbers of s100 family proteins are almost located at 1q21 as a cluster. S100B is glial-specific and is expressed primarily by astrocytes. Not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca 2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100B Antibody (NBP2-45267) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100B Antibody (NBP2-45267) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-45267AF350
Alexa Fluor 405 NBP2-45267AF405
Alexa Fluor 488 NBP2-45267AF488
Alexa Fluor 532 NBP2-45267AF532
Alexa Fluor 594 NBP2-45267AF594
Alexa Fluor 647 NBP2-45267AF647
Alexa Fluor 700 NBP2-45267AF700
Alexa Fluor 750 NBP2-45267AF750
Allophycocyanin NBP2-45267APC
Biotin NBP2-45267B
DyLight 350 NBP2-45267UV
DyLight 405 NBP2-45267V
DyLight 488 NBP2-45267G
DyLight 550 NBP2-45267R
DyLight 594 NBP2-45267DL594
DyLight 650 NBP2-45267C
DyLight 680 NBP2-45267FR
DyLight 755 NBP2-45267IR
HRP NBP2-45267H
Janelia Fluor 549 NBP2-45267JF549
Janelia Fluor 646 NBP2-45267JF646
PE NBP2-45267PE
PerCP NBP2-45267PCP

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Blogs on S100B.

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients
By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an...  Read full blog post.

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Bioinformatics

Gene Symbol S100B
Uniprot