S100B Antibody (15F4NB)

Images

 
Western Blot: S100B Antibody (15F4NB) [NBP2-45224] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and ...read more
Immunocytochemistry/ Immunofluorescence: S100B Antibody (15F4NB) [NBP2-45224] - Immunocytochemistry/ Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% ...read more
Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) [NBP2-45224] - Analysis using Azide Free version of NBP2-45224. IHC S100B in human prostate
Flow Cytometry: S100B Antibody (15F4NB) [NBP2-45224] - An intracellular stain was performed on SK-MEL-28 cells with S100b (15F4NB) antibody NBP2-45224R (blue) and a matched isotype control (orange). Cells were fixed ...read more
Immunocytochemistry/ Immunofluorescence: S100B Antibody (15F4NB) [NBP2-45224] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) [NBP2-45224] - IHC analysis of S100B (15F4BN) in human brain tissue.
Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) [NBP2-45224] - IHC analysis of S100B (15F4NB) in human prostate tissue.
Immunohistochemistry: S100B Antibody (15F4NB) [NBP2-45224] - Analysis using Azide Free version of NBP2-45224. IHC S100B (15F9NB) in human brain.
Flow Cytometry: S100B Antibody (15F4NB) [NBP2-45224] - Analysis using the Azide Free version of NBP2-45224. Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution ...read more
Flow Cytometry: S100B Antibody (15F4NB) [NBP2-45224] - An intracellular stain was performed on Jurkat cells with S100b (15F4NB) antibody NBP2-45224AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, Po, Bv, Eq, Pm, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
15F4NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

S100B Antibody (15F4NB) Summary

Immunogen
Partial recombinant human S100B protein (amino acids 2-92) [UniProt P04271]
Predicted Species
Mouse (99%), Porcine (97%), Rabbit (100%), Equine (100%), Primate (100%), Bovine (97%), Sheep (99%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:500
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28352122).
Reviewed Applications
Read 1 Review rated 3
using
NBP2-45224 in the following applications:

Publications
Read Publication using
NBP2-45224 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28352122).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S100B Antibody (15F4NB)

  • beta (neural)
  • NEF
  • S100 beta
  • S100 calcium binding protein B
  • S100 calcium-binding protein B
  • S100 calcium-binding protein, beta (neural)
  • S-100 calcium-binding protein, beta chain, 10protein S100-B
  • S-100 protein beta chain
  • S-100 protein subunit beta
  • S100
  • S100B
  • S100beta

Background

S100B belongs to the S100 family, which containing 2 EF-hand calcium-binding motifs. In humans, S100B protein is encoded by the S100P gene located at 4q16, but genes that encode other numbers of s100 family proteins are almost located at 1q21 as a cluster. S100B is glial-specific and is expressed primarily by astrocytes. Not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca 2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100B Antibody (NBP2-45224)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC-FR.


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IHC-FR
(1)
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Review for S100B Antibody (NBP2-45224) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-45224:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Sitara Sankar
WB Mouse 07/20/2017
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Summary

ApplicationWestern Blot
Sample Testedprimary astrocytes
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100B Antibody (NBP2-45224) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-45224AF405
Alexa Fluor 488 Labeled NBP2-45224AF488
Alexa Fluor 647 Labeled NBP2-45224AF647
Alexa Fluor 700 Labeled NBP2-45224AF700
Allophycocyanin Labeled NBP2-45224APC
Biotin Labeled NBP2-45224B
DyLight 350 Labeled NBP2-45224UV
DyLight 405 Labeled NBP2-45224V
DyLight 488 Labeled NBP2-45224G
DyLight 550 Labeled NBP2-45224R
DyLight 650 Labeled NBP2-45224C
DyLight 680 Labeled NBP2-45224FR
DyLight 755 Labeled NBP2-45224IR
HRP Labeled NBP2-45224H
PE Labeled NBP2-45224PE
PerCP Labeled NBP2-45224PCP

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Recent Reviews

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1
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Sitara Sankar
07/20/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol S100B
Entrez
Uniprot