S100B Antibody (1046211) [Unconjugated]

S100B was detected in immersion fixed WM‑115 human malignant melanoma cell line (positive staining) and MCF‑7 human breast cancer cell line (negative staining) using Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18204) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1046211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

S100B Antibody (1046211) [Unconjugated] Summary

• Immunogen: Human S100B synthetic peptide
  Accession # P04271
• Specificity: Detects human S100B in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100B Antibody (1046211) [Unconjugated]

• beta (neural)
• NEF
• S100 beta
• S100 calcium binding protein B
• S100 calcium-binding protein B
• S100 calcium-binding protein, beta (neural)
• S-100 calcium-binding protein, beta chain, 10protein S100-B
• S-100 protein beta chain
• S-100 protein subunit beta
• S100
• S100B
• S100beta

Background

S100B belongs to the S100 subgroup of the EF-hand family of Ca²⁺ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P04271()