S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3059) for 3 hours at ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for S100A8 Antibody [Biotin]
Calprotectin L1L subunit
Cystic fibrosis antigen
Leukocyte L1 complex light chain
Migration inhibitory factor-related protein 8
MRP8S100 calcium binding protein A8 (calgranulin A)
S100 calcium binding protein A8
S100 calcium-binding protein A8 (calgranulin A)
S100 calcium-binding protein A8
Urinary stone protein band A
Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa
member of the S100 family of calcium-binding proteins. S100A8 contains
short sequential modules, including an N-terminal alpha -helix, a Ca++-binding
EF-hand segment, a short central linker region, a second EF-hand
segment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizes
with S100A9. In the presence of Ca++, the heterodimers form
heterotetramers. The dimeric complex is found both intracellularly and
extracellularly. It binds to heparan sulfate and is chemotactic for
PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80%
and 57% identical to that of rat and human S100A8, respectively.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for S100A8 Antibody (BAF3059). (Showing 1 - 2 of 2 FAQs).
Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.