Western Blot: Exosome Component 9 Antibody [NBP1-71702] - Analysis of EXOSC9 in A. HepG2 cell lysate and B. MCF7 cell lysate.
Immunocytochemistry/ Immunofluorescence: Exosome Component 9 Antibody [NBP1-71702] - EXOSC9 antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and ...read more
Immunohistochemistry: Exosome Component 9 Antibody [NBP1-71702] - Staining of EXOSC9 in mouse prostate.
Simple Western: Exosome Component 9 Antibody [NBP1-71702] - Simple Western lane view shows a specific band for Exosome Component 9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions ...read more
This EXOSC9 antibody is useful for IHC-P, ICC/IF and Western blot, where a band is seen ~75 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. NBP1-71702 has also been successfully used for IP as reported by a customer review. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27543448).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Exosome component 9 is part of the 3' -> 5' exoribonuclease complex that helps to degrade unstable mRNA. It binds to ARE-containing RNAs and performs 3' end trimming and degradation. These activities lead toward the maturation of RNA into more stable forms, which then become involved in transcription and translation. In the related Arabidopsis equivalent, loss of function of this protein is a lethal mutation. In ruminants, EXOSC9 is differentially expressed throughout the gut at different stages of development. EXOSC9 seems to be important in the degradation of histone mRNA and may be important in some downstream epigenetic effects.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
1. After Western blotting, membrane was blocked for 30 minutes in 5% milk dissolved in PBS containing 0.1% Tween 20 (PBST).2. Membrane was blotted with primary antibody diluted in PBST at 4C overnight.
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