Exosome Component 9 Antibody

Images

 
Western Blot: Exosome Component 9 Antibody [NBP1-71702] - Analysis of EXOSC9 in A. HepG2 cell lysate and B. MCF7 cell lysate.
Immunocytochemistry/ Immunofluorescence: Exosome Component 9 Antibody [NBP1-71702] - EXOSC9 antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and ...read more
Immunohistochemistry: Exosome Component 9 Antibody [NBP1-71702] - Staining of EXOSC9 in mouse prostate.
Simple Western: Exosome Component 9 Antibody [NBP1-71702] - Simple Western lane view shows a specific band for Exosome Component 9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, Ch, BvSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.6 mg/ml

Exosome Component 9 Antibody Summary

Immunogen
A genomic peptide made to an internal region of human Exosome Component 9 (within residues 250-439). [Swiss-Prot Q06265]
Localization
Cytoplasm. Nucleus - nucleolus.
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOSC9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Simple Western 1:1000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
Application Notes
This EXOSC9 antibody is useful for IHC-P, ICC/IF and Western blot, where a band is seen ~75 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. NBP1-71702 has also been successfully used for IP as reported by a customer review. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27543448).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
MCF-7 Lysate (NB820-59465)
HepG2 Lysate (NBP1-42569)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-71702 in the following applications:

Publications
Read Publications using
NBP1-71702 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Human, Mouse and Chicken. Immunogen has 83% identity to rat and 90% identity to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Exosome Component 9 Antibody

  • Autoantigen PM/Scl 1
  • EC 3.1.13
  • exosome component 9Polymyositis/scleroderma autoantigen 75 kDa
  • p6
  • PM/Scl-75P75 polymyositis-scleroderma overlap syndrome-associated autoantigen
  • PMSCL175kD
  • polymyositis/scleroderma autoantigen 1 (75kD)
  • Polymyositis/scleroderma autoantigen 1
  • polymyositis/scleroderma autoantigen 1, 75kDa

Background

Exosome component 9 is part of the 3' -> 5' exoribonuclease complex that helps to degrade unstable mRNA. It binds to ARE-containing RNAs and performs 3' end trimming and degradation. These activities lead toward the maturation of RNA into more stable forms, which then become involved in transcription and translation. In the related Arabidopsis equivalent, loss of function of this protein is a lethal mutation. In ruminants, EXOSC9 is differentially expressed throughout the gut at different stages of development. EXOSC9 seems to be important in the degradation of histone mRNA and may be important in some downstream epigenetic effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Exosome Component 9 Antibody (NBP1-71702)(3)

Reviews for Exosome Component 9 Antibody (NBP1-71702) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.
We have 2 reviews tested in 2 application: WB, IP.

Reviews using NBP1-71702:
Filter by Applications
WB
(1)
IP
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Exosome Component 9 NBP1-71702
Enlarge
reviewed by:
Hsin Jing-Ping
WB Other 10/18/2013
View

Summary

ApplicationWestern Blot
SpeciesOther
Special Applications1. After Western blotting, membrane was blocked for 30 minutes in 5% milk dissolved in PBS containing 0.1% Tween 20 (PBST).2. Membrane was blotted with primary antibody diluted in PBST at 4C overnight.
Immunoprecipitation Exosome Component 9 NBP1-71702
Enlarge
reviewed by:
Patricia Richard
IP Human 08/02/2012
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa, U87 whole cell extract and nuclear extract
SpeciesHuman
LotA1
CommentsThis antibody also works in Western Blot and ChIP

Blocking

Blocking Details20 min in PBST, 5% milk

Primary Anitbody

Dilution Ratio1:1000 in PBST 5% milk O/N at 4 degrees

Secondary Antibody

Secondary Descriptionanti-Rabbit IgG
Secondary Manufacturer Cat#A6154, Sigma
Secondary Concentration1:10000

Details

Detection NotesECL, exposure time 30 sec to 2 min
Immunoprecipitation Detailsprotein G sepharose, O/N at 4 degrees

Comments

CommentsThis antibody also works in Western Blot and ChIP

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Exosome Component 9 Antibody (NBP1-71702) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71702AF405
Alexa Fluor 488 Labeled NBP1-71702AF488
Alexa Fluor 647 Labeled NBP1-71702AF647
Alexa Fluor 700 Labeled NBP1-71702AF700
Biotin Labeled NBP1-71702B
DyLight 405 Labeled NBP1-71702V
DyLight 488 Labeled NBP1-71702G
DyLight 550 Labeled NBP1-71702R
DyLight 650 Labeled NBP1-71702C
FITC Labeled NBP1-71702F
HRP Labeled NBP1-71702H

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Bioinformatics Tool for Exosome Component 9 Antibody (NBP1-71702)

Discover related pathways, diseases and genes to Exosome Component 9 Antibody (NBP1-71702). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Hsin Jing-Ping
10/18/2013
Application: WB
Species: Other

Patricia Richard
08/02/2012
Application: IP
Species: Human

Bioinformatics

Gene Symbol EXOSC9
Entrez
Uniprot