Exosome Component 9 Antibody - BSA Free

Western Blot: Exosome Component 9 Antibody [NBP1-71702] - Analysis of EXOSC9 in A. HepG2 cell lysate and B. MCF7 cell lysate.

Immunocytochemistry/Immunofluorescence: Exosome Component 9 Antibody [NBP1-71702] - EXOSC9 antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunohistochemistry: Exosome Component 9 Antibody [NBP1-71702] - Staining of EXOSC9 in mouse prostate.

Simple Western: Exosome Component 9 Antibody [NBP1-71702] - Simple Western lane view shows a specific band for Exosome Component 9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Ch, Bv
• Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Exosome Component 9 Antibody - BSA Free Summary

• Immunogen: Partial recombinant protein made to an internal region of human Exosome Component 9 (within residues 250-439). [Swiss-Prot Q06265]
• Localization: Cytoplasm. Nucleus - nucleolus.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXOSC9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 27543448)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation reported by customer review
  • Simple Western 1:1000
  • Western Blot 1:5000
• Application Notes: In Western blot, a band is seen ~75 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 62 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  MCF-7 Whole Cell Lysate
  HepG2 Whole Cell Lysate

Reactivity Notes

Immunogen has 83% identity to rat and 90% identity to bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Exosome Component 9 Antibody - BSA Free

• Autoantigen PM/Scl 1
• EC 3.1.13
• exosome complex component RRP45
• exosome complex exonuclease RRP45
• Exosome Component 9
• p5
• p6
• P75 polymyositis-scleroderma overlap syndrome associated autoantigen
• PCH1D
• PM/Scl-75
• PMSCL autoantigen, 75kD
• PMSCL1
• polymyositis/scleroderma autoantigen 1 (75kD)
• Polymyositis/scleroderma autoantigen 1
• polymyositis/scleroderma autoantigen 1, 75kDa
• RRP45
• Rrp45p

Background

Exosome component 9 is part of the 3' -> 5' exoribonuclease complex that helps to degrade unstable mRNA. It binds to ARE-containing RNAs and performs 3' end trimming and degradation. These activities lead toward the maturation of RNA into more stable forms, which then become involved in transcription and translation. In the related Arabidopsis equivalent, loss of function of this protein is a lethal mutation. In ruminants, EXOSC9 is differentially expressed throughout the gut at different stages of development. EXOSC9 seems to be important in the degradation of histone mRNA and may be important in some downstream epigenetic effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Exosome Component 9 Antibody (NBP1-71702)(4)

Publications using NBP1-71702

• McIver SC, Katsumura KR, Davids E et al. Exosome complex orchestrates developmental signaling to balance proliferation and differentiation during erythropoiesis. Elife. 2016-08-20 [PMID: 27543448] (Chemotaxis, Mouse)
• Morales JC, Richard P, Patidar PL et al. XRN2 Links Transcription Termination to DNA Damage and Replication Stress PLoS Genet. 2016-07-01 [PMID: 27437695] (Western Blot, Human)
• Hsin JP, Li W, Hoque M et al. RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells. Elife (Cambridge) 2014-06-04 [PMID: 24842995] (WB, Chicken)
  
  Details:
  EXOSC3 antibody used for WB on lysates of 26r (DT40 cells derived Rpb1 derivative containing a CTD with 26 YSPTSPS repeats) and 25F+Y cells (Rpb1-Y1F derivative in which only a single F, in the C terminal-most heptad, was changed back to Y). WB data shown in Supplement Figure 4.
• Richard P, Feng S, Manley JL. A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage. Genes Dev. 2013-10-15 [PMID: 24105744] (WB, Human)

Reviews for Exosome Component 9 Antibody (NBP1-71702) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Other.

reviewed by: Hsin Jing-Ping WB Other 10/18/2013

Summary

• Application: Western Blot
• Species: Other

reviewed by: Patricia Richard IP Human 08/02/2012

Summary

• Application: Immunoprecipitation
• Sample Tested: HeLa, U87 whole cell extract and nuclear extract
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: EXOSC9
• Entrez:
  • 5393(Human)
  • 50911(Mouse)
• Uniprot:
  • Q06265(Human)
  • Q9JHI7(Mouse)