S100A7/Psoriasin Antibody (9F3) - Azide Free

• Reactivity: Hu, Rt, Ca, Rb, Sh
• Applications: WB, ELISA, Func, ICC/IF, IHC
• Clone: 9F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

S100A7/Psoriasin Antibody (9F3) - Azide Free Summary

• Description: Novus Biologicals Mouse S100A7/Psoriasin Antibody (9F3) - Azide Free (NBP3-11185) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length recombinant human S100A7
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100A7
• Purity: Protein G purified
• Endotoxin Note: <24 EU/mg

Applications/Dilutions

• Dilutions:
  • Functional
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for S100A7/Psoriasin Antibody (9F3) - Azide Free

• HID 5
• HID5
• HID-5
• protein S100-A7
• PSOR1
• psoriasin 1
• Psoriasin
• S100 calcium binding protein A7 (psoriasin 1)
• S100 calcium binding protein A7
• S100 calcium-binding protein A7 (psoriasin 1)
• S100 calcium-binding protein A7
• S100A7
• S100A7c

Background

Antibody 9F3 recognizes human S100A7 (Psoriasin). S100A7 is a 11.4kDa member of the S100 gene family, which are EF-hand Ca 2+-binding proteins. They are involved in a broad range of intra- and extracellular processes like regulation of calcium homeostasis, cell proliferation, differentiation, apoptosis, immune responses, antimicrobial activity, chemotaxis and inflammation. S100 proteins have a fundamental structural unit which is a highly integrated dimer, some as homodimer and others as heterodimer. S100A7 functions as a homodimer and binds two Zn2+ ions. These ions stabilize the dimer and mediate the function of S100A7 during infection. Extracellular S100A7 functions as a DAMP during infection. S100A7 limits bacterial infection through metal limitation. Furthermore, S100A7 interacts with RAGE which drives an NF-kappaB mediated proinflammatory response and recruitment of neutrophils. S100A7 was first discovered as a protein abundantly expressed in psoriatic keratinocytes. Possibly, S100A7 adheres directly to, and reduces survival of, pathogens found on the epidermis and acts as a physical barrier. S100A7 also seems to be involved with breast cancer and squamous cell carcinoma. In vitro it was found to confer a more aggressive behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100A7