FABP5/E-FABP Antibody [Unconjugated]

Images

 
Western blot shows lysates of human placenta tissue, mouse liver tissue, rat brain tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FABP5/E‑FABP Antigen ...read more
FABP5/E‑FABP was detected in immersion fixed paraffin-embedded sections of mouse skin (ear) tissue using Goat Anti-Human/Mouse/Rat FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) ...read more
Simple Western lane view shows lysates of human placenta and mouse liver, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at approximately 20 kDa (as indicated) using 10 µg/mL of Goat ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E‑FABP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of ...read more
JunB cKOs display impaired skin homeostasis. a Representative photomicrographs with immunostaining of FABP5 (red), indicative of sebaceous glands, in dorsal back skin from wild type and JunB cKO mice. Nuclei stained ...read more
JunB expression during skin maturation and upon stress. a Representative microphotographs with double immunostaining for JunB (green) and FABP5 (red), indicative of sebaceous glands, in skin derived from 1, 3, and 5 ...read more
De novo formation of sebaceous glands in JunB cKO wounds. a Representative H&E photomicrographs of 30 days old wound restoration tissue depicting ectopic sebaceous glands in JunB cKO mice. Inset showing magnified view. ...read more
JunB expression during skin maturation and upon stress. a Representative microphotographs with double immunostaining for JunB (green) and FABP5 (red), indicative of sebaceous glands, in skin derived from 1, 3, and 5 ...read more
Notch inhibition restored epidermal homeostasis in JunB cKOs. a Cartoon depicting experimental design and administration schedule of the Notch inhibitor DBZ or vehicle in JunB cKO mice. b Representative H&E staining and ...read more
Notch inhibition restored epidermal homeostasis in JunB cKOs. a Cartoon depicting experimental design and administration schedule of the Notch inhibitor DBZ or vehicle in JunB cKO mice. b Representative H&E staining and ...read more
JunB expression during skin maturation and upon stress. a Representative microphotographs with double immunostaining for JunB (green) and FABP5 (red), indicative of sebaceous glands, in skin derived from 1, 3, and 5 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

FABP5/E-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse FABP5/E‑FABP
Met1-Gln135
Accession # Q05816
Specificity
Detects mouse FABP5/E‑FABP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF1476 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP5/E-FABP Antibody [Unconjugated]

  • EFABP
  • E-FABP
  • E-FABPPAFABP
  • FABP5
  • fatty acid binding protein 5 (psoriasis-associated)
  • Fatty acid-binding protein 5
  • PA-FABPepidermal
  • Psoriasis-associated fatty acid-binding protein homolog

Background

FABP5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FABP5/E-FABP Antibody (AF1476)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC
(6)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(1)
Mouse
(12)
Rat
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using AF1476 Applications Species
M Fauzan, S Oubraim, M Yu, ST Glaser, M Kaczocha, S Haj-Dahman Fatty Acid-Binding Protein 5 Modulates Brain Endocannabinoid Tone and Retrograde Signaling in the Striatum Frontiers in Cellular Neuroscience, 2022-07-07;16(0):936939. 2022-07-07 [PMID: 35875351] (IHC, Mouse) IHC Mouse
DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain Oncogene, 2022-06-02;12(1):9241. 2022-06-02 [PMID: 35655086] (IHC, Mouse) IHC Mouse
C Beuker, D Schafflick, JK Strecker, M Heming, X Li, J Wolbert, A Schmidt-Po, C Thomas, T Kuhlmann, I Aranda-Par, N A-Gonzalez, PA Kumar, Y Werner, E Kilic, DM Hermann, H Wiendl, R Stumm, GMZ Hörste, J Minnerup Stroke induces disease-specific myeloid cells in the brain parenchyma and pia Nature Communications, 2022-02-17;13(1):945. 2022-02-17 [PMID: 35177618] (IHC, Human, Mouse) IHC Human, Mouse
Y Li, S Wang, Z Xing, Y Niu, Z Liao, Y Lu, J Qiu, J Zhang, C Wang, L Dong Destructing biofilms by cationic dextran through phase transition Carbohydrate polymers, 2021-10-23;279(0):118778. 2021-10-23 [PMID: 34980345] (IHC, Mouse) IHC Mouse
Di Yin, Jiaqing Hao, Rong Jin, Yanmei Yi, Sobha R. Bodduluri, Yuan Hua et al. Epidermal Fatty Acid‒Binding Protein Mediates Depilatory-Induced Acute Skin Inflammation Journal of Investigative Dermatology 2022-07-01 [PMID: 34942197]
Bénédicte Oulès, Emanuel Rognoni, Esther Hoste, Georgina Goss, Ryan Fiehler, Ken Natsuga et al. Mutant Lef1 controls Gata6 in sebaceous gland development and cancer The EMBO Journal 2019-05-02 [PMID: 30886049]
Francesco Napoletano, Olga Baron, Peter Vandenabeele, Bertrand Mollereau, Manolis Fanto Intersections between Regulated Cell Death and Autophagy Trends in Cell Biology 2019-04-01 [PMID: 30665736]
Austin Moore, Linwei Wu, Jen‐Chieh Chuang, Xuxu Sun, Xin Luo, Purva Gopal et al. Arid1a Loss Drives Nonalcoholic Steatohepatitis in Mice Through Epigenetic Dysregulation of Hepatic Lipogenesis and Fatty Acid Oxidation Hepatology 2019-05-01 [PMID: 30584660]
K Singh, E Camera, L Krug, A Basu, RK Pandey, S Munir, M Wlaschek, S Kochanek, M Schorpp-Ki, M Picardo, P Angel, C Niemann, P Maity, K Scharffett JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin Nat Commun, 2018-08-24;9(1):3425. 2018-08-24 [PMID: 30143626] (IHC-P, Mouse) IHC-P Mouse
Yuwen Zhang, Jiaqing Hao, Jun Zeng, Qiang Li, Enyu Rao, Yanwen Sun et al. Epidermal fatty acid binding protein prevents chemical-induced skin tumorigenesis by regulation of TPA-induced IFN/p53/SOX2 pathway in keratinocytes Journal of Investigative Dermatology 2018-09-01 [PMID: 29559340]
Show All 20 Publications.

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Bioinformatics

Gene Symbol FABP5
Uniprot