FABP5/E-FABP Antibody [Unconjugated]

Images

 
Western blot shows lysates of human placenta tissue, mouse liver tissue, rat brain tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FABP5/E‑FABP Antigen ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E‑FABP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat ...read more
FABP5/E‑FABP was detected in immersion fixed paraffin-embedded sections of mouse skin (ear) tissue using Goat Anti-Human/Mouse/Rat FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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FABP5/E-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse FABP5/E‑FABP
Met1-Gln135
Accession # Q05816
Specificity
Detects mouse FABP5/E‑FABP in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse FABP4 and recombinant human (rh) FABP3 is observed and less than 1% cross-reactivity with recombinant rat (rr) FABP2 and rrFABP1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Knockout Validated
Control
FABP5/E-FABP Knockout HeLa Cell Lysate
Publications
Read Publications using
AF1476 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP5/E-FABP Antibody [Unconjugated]

  • EFABP
  • E-FABP
  • E-FABPPAFABP
  • FABP5
  • fatty acid binding protein 5 (psoriasis-associated)
  • Fatty acid-binding protein 5
  • PA-FABPepidermal
  • Psoriasis-associated fatty acid-binding protein homolog

Background

FABP5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP5/E-FABP Antibody (AF1476)(9)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Western Blot
(7)
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Mouse
(8)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF1476 Applications Species
K Singh, E Camera, L Krug, A Basu, RK Pandey, S Munir, M Wlaschek, S Kochanek, M Schorpp-Ki, M Picardo, P Angel, C Niemann, P Maity, K Scharffett JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin Nat Commun, 2018;9(1):3425. 2018 [PMID: 30143626] (IHC-P, Mouse) IHC-P Mouse
Yuwen Zhang Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide Production J. Immunol, 2016;0(0):. 2016 [PMID: 27920274] (Western Blot, Mouse) Western Blot Mouse
Kretzschmar K, Cottle D, Donati G, Chiang M, Quist S, Gollnick H, Natsuga K, Lin K, Watt F BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions. Stem Cell Reports, 2014;3(4):620-33. 2014 [PMID: 25358790] (IHC, Rat) IHC Rat
Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein K, Liu S, Sauter E, Cleary M, Li B Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophages. Cancer Res, 2014;74(11):2986-98. 2014 [PMID: 24713431] (Western Blot, Mouse) Western Blot Mouse
Yu S, Levi L, Casadesus G, Kunos G, Noy N Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) in the brain. J Biol Chem, 2014;289(18):12748-58. 2014 [PMID: 24644281] (Western Blot, Mouse) Western Blot Mouse
Yu S, Levi L, Siegel R, Noy N Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta). J Biol Chem, 2012;287(50):42195-205. 2012 [PMID: 23105114] (Western Blot, Mouse) Western Blot Mouse
Li C, Wang W, Wang H, Zhong Y, Di J, Lin Y Proteomic analysis of proteins differentially expressed in uterine lymphocytes obtained from wild-type and NOD mice. J. Cell. Biochem., 2009;108(2):447-57. 2009 [PMID: 19623579] (Western Blot, Mouse) Western Blot Mouse
Berry DC, Noy N All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptor beta/delta and retinoic acid receptor. Mol. Cell. Biol., 2009;29(12):3286-96. 2009 [PMID: 19364826] (Western Blot, Mouse) Western Blot Mouse
Witting SR, Brown M, Saxena R, Nabinger S, Morral N Helper-dependent adenovirus-mediated short hairpin RNA expression in the liver activates the interferon response. J. Biol. Chem., 2007;283(4):2120-8. 2007 [PMID: 18025090] (Western Blot, Mouse) Western Blot Mouse

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Control Lysate(s)

Secondary Antibodies

 

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Other Available Formats

Biotin BAF1476

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Bioinformatics

Gene Symbol FABP5
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