JAB1 Antibody (2A10.8)


Western Blot: JAB1 Antibody (2A10) [NB120-495] - Sample (50 ug of whole cell lysate) A: Rat liver 10% SDS PAGE diluted at 1:1000
Immunohistochemistry: JAB1 Antibody (2A10) [NB120-495]
Western Blot: JAB1 Antibody (2A10) [NB120-495] - Jab1 antibody [2A10.8] Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE JAB1 Antibody diluted at 1:10000
Western Blot: JAB1 Antibody (2A10) [NB120-495] - Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 ...read more

Product Details

Reactivity Hu, Mu, Rt, DrSpecies Glossary
Applications WB, ICC/IF, IHC, IP

Order Details

JAB1 Antibody (2A10.8) Summary

The full-length mouse JAB-1 gene expressed in E. coli.
Cytoplasmic and Nuclear
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 0.5-1 ug/ml
  • Western Blot 1:500-1:3000
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB120-495 in the following applications:

  • 7 publications
  • IP
    1 publication
  • WB
    8 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No Preservative
Protein G purified

Alternate Names for JAB1 Antibody (2A10.8)

  • COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
  • COP9 signalosome complex subunit 5
  • COPS5
  • CSN5
  • CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5
  • EC 3.4
  • JAB1
  • JAB138 kDa Mov34 homolog
  • Jun activation domain-binding protein 1
  • MOV-34
  • SGN5
  • SGN5MGC3149
  • Signalosome subunit 5


Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported. JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1 induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JAB1 Antibody (NB120-495)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB120-495 Applications Species
Pines A, Schiffmacher DL, Lee SH et al. DDA1, a novel factor in transcription-coupled repair, modulates CRL4CSA dynamics at DNA damage-stalled RNA polymerase II Research square 2023-10-12 [PMID: 37886519] (IP) IP
Israel S, Drexler Hca, Fuellen G, Boiani M The COP9 signalosome subunit 3 is necessary for early embryo survival by way of a stable protein deposit in mouse oocytes Molecular human reproduction 2021-07-15 [PMID: 34264319]
Karpiyevich M, Adjalley S, Mol M et al. Nedd8 hydrolysis by UCH proteases in Plasmodium parasites PLoS Pathog. 2019-10-01 [PMID: 31658303] (WB, Human) WB Human
Wang R, Wang H, Carrera I et al. COPS5 Overexpression Increases Amyloid Plaque Burden, Decreases Spinophilin-Immunoreactive-Puncta and Exacerbates Learning and Memory Deficits in the Mouse Brain J. Biol. Chem. 2015-02-20 [PMID: 25713139]
Wang H, Dey D, Carrera I et al. COPS5 (Jab1) increases ?-site processing of amyloid precursor protein and amyloid ? peptide generation by stabilizing RanBP9 protein levels. J Biol Chem 2013-08-07 [PMID: 23926111] (ICC/IF, WB, Mouse, Human) ICC/IF, WB Mouse, Human
Merk M, Zierow S, Leng L et al. The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF). Proc Natl Acad Sci USA 2011-08-01 [PMID: 21817065] (WB, ICC/IF, Human) WB, ICC/IF Human
McCall CM, Miliani de Marval PL, Chastain PD 2nd et al. Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development. Mol Cell Biol 2008-09-01 [PMID: 18606781] (ICC/IF, WB, Human) ICC/IF, WB Human
Yun J, Tomida A, Andoh T et al. Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5. J Biol Chem 2004-07-01 [PMID: 15126503] (WB, ICC/IF, Human) WB, ICC/IF Human
Doronkin S, Djagaeva I, Beckendorf SK. CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint. Development 2002-11-01 [PMID: 12397113] (ICC/IF, WB, Human) ICC/IF, WB Human
Korbonits M, Chahal HS, Kaltsas G et al. Expression of phosphorylated p27(kip1) protein and Jun activation domain-binding protein 1 in human pituitary tumors. J Clin Endocrinol Metab 2002-06-01 [PMID: 12050228] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 11 Publications.

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Gene Symbol COPS5