S100A2 Antibody (CPTC-S100A2-2)

Western Blot: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Western Blot Analysis of A431 cell lysate using S100A2 Antibody (CPTC-S100A2-2).

Immunocytochemistry/Immunofluorescence: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Immunofluorescence Analysis of A549 cells labeling S100A2 with S100A2 Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)

Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Formalin-fixed, paraffin-embedded human Placenta stained with S100A2 Antibody (CPTC-S100A2-2).

Flow Cytometry: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Flow Cytometric Analysis of trypsinised HeLa cells. S100A2 Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Formalin-fixed, paraffin-embedded human Skin stained with S100A2 Antibody (CPTC-S100A2-2).

Protein Array: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A2 Antibody (CPTC-S100A2-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, MA
• Clone: CPTC-S100A2-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

S100A2 Antibody (CPTC-S100A2-2) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79899)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human S100A2 protein (Uniprot: P29034)
• Localization: Nucleus, Cytoplasm
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100A2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 11.12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for S100A2 Antibody (CPTC-S100A2-2)

• CAN19
• CAN19protein S100-A2
• MGC111539
• S100 calcium binding protein A2
• S100 calcium-binding protein A2Protein S-100L
• S100A2
• S100L

Background

S100 alpha 2 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100A2
• Uniprot:
  • P29034()
  • P29034()
  • P29034()
  • P29034()