S100A11 Antibody

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human S100A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4874) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A11 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

S100A11 Antibody Summary

• Immunogen: E. coli-derived recombinant human S100A11
  Ala2-Thr105
  Accession # P31949
• Specificity: Detects human S100A11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) S100A1, A2, A7, A8, A10, A13, A16, rhS100B, rhS100P, recombinant mouse (rm) S100A6, and rmS100A10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100A11
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A11 Antibody

• Calgizzarin
• Metastatic lymph node gene 70 protein
• MLN 70
• MLN70
• protein S100-A11
• Protein S100-C
• S100 calcium binding protein A11
• S100 calcium-binding protein A11 (calgizzarin)
• S100 calcium-binding protein A11
• S100A11
• S100C
• S100CS100 calcium binding protein A11 (calgizzarin)

Background

S100A11 (also S100C and calgizzarin) is a 10‑12 kDa member of the S100 family, EF-hand superfamily of Ca‑binding proteins. It is produced by smooth muscle and keratinocytes. Intracellularly, it suppresses growth; extracellularly, it exists as both a monomer, homodimer, and heterodimer with S100B, binds to RAGE, induces EGF, and promotes cell growth. Human S100A11 is 105 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑49 and 55‑90) and one high-affinity Ca‑binding site (aa 68‑79), and binds annexin I with its C-terminal half. There may be one alternative splice form that apparently contains a series of mutations over aa 61‑79 and shows 90% overall aa identity to the standard form. Full-length human S100A11 shares 78% and 82% aa identity with mouse and porcine S100A11, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A11 Antibody (AF4874)(1)

Publication using AF4874

• H Sato, M Sakaguchi, H Yamamoto, S Tomida, K Aoe, K Shien, T Yoshioka, K Namba, H Torigoe, J Soh, K Tsukuda, H Tao, K Okabe, S Miyoshi, HI Pass, S Toyooka Therapeutic potential of targeting S100A11 in malignant pleural mesothelioma Oncogenesis, 2018-01-24;7(1):11. 2018-01-24 [PMID: 29362358] (IHC, Human)

Bioinformatics

• Gene Symbol: S100A11
• Uniprot:
  • P31949()