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S100A7/Psoriasin Antibody (47C1068) - BSA Free

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Western Blot: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Psoriasin in A) MCF10A and B) MCF7 cell lysate using psoriasin antibody at 1 ug/mL.
Biological Strategies: Immunohistochemistry: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Activities of proinflammatory cytokines on the differentiation of Reconstituted Human Epidermis. RHE have been ...read more
Immunocytochemistry/ Immunofluorescence: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - S100A7/psoriasin detection was performed on (A) untreated and (B) LPS-treated (100 ng/mL, 21 hrs) HOPE-fixed NCI-H727 cells ...read more
Immunohistochemistry: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Inhibition of KDM expression in vivo. Skin biopsies from normal control skin (Cont) or lesional psoriatic skin (Pso) were collected. Skin ...read more
Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Staining of human skin. FFPE tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/mL.
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis of FFPE tissue section of human lymph node cancer using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Human breast tissue stained with psoriasin antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with ...read more
Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control ...read more
Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Image shows a specific band for S100A7/Psoriasin in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Image shows a specific band for Psoriasin/S100A7 in 0.05 mg/mL of MCF-7 lysate. This experiment was ...read more
S100A7 activated p53 related anti-proliferative pathways. (A) Log2 fold change of mRNA expression of p53 pathway factors after S100A7 overexpression. (B) WB of p53 and S100A7 in MCF7-p53-CKD transfected with S100A7 or ...read more
PINK1 translation is critical for the accumulation of PINK1 upon mitochondrial damage. aPINK1 mRNA levels were not altered by mitochondrial damage. HeLa cells were incubated with 10 μM CCCP or combination of 10 μM ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, MS
Clone
47C1068
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

S100A7/Psoriasin Antibody (47C1068) - BSA Free Summary

Immunogen
This antibody was developed against recombinant psoriasin/HID-5 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100A7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy reported in scientific literature (PMID 26225121)
  • Flow (Intracellular)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation reported in scientific literature (PMID 26225121)
  • Simple Western 1:500
  • Western Blot 1-3 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 4
using
NB100-56559 in the following applications:

Publications
Read Publications using
NB100-56559 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:25622979).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S100A7/Psoriasin Antibody (47C1068) - BSA Free

  • HID 5
  • HID5
  • HID-5
  • protein S100-A7
  • PSOR1
  • psoriasin 1
  • Psoriasin
  • S100 calcium binding protein A7 (psoriasin 1)
  • S100 calcium binding protein A7
  • S100 calcium-binding protein A7 (psoriasin 1)
  • S100 calcium-binding protein A7
  • S100A7
  • S100A7c

Background

Psoriasin/HID-5 was originally identified as an up-regulated protein in psoriatic keratinocytes (1). Subsequently, this protein was found to have elevated expression in abnormally differentiating primary keratinocytes and in various carcinomas, including ductal carcinoma in situ (2-3). Psoriasin/HID-5 may play a role in the establishment or maintenance of these pathologies, and may serve as a diagnostic marker of particular tumor types or abnormally proliferative epithelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A7/Psoriasin Antibody (NB100-56559)(50)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(6)
IF/IHC
(7)
IHC-Fr
(2)
IHC-P
(12)
IP
(1)
WB
(13)
All Applications
Filter By Species
Human
(29)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using NB100-56559 Applications Species
Todorovic, McDonald HA, Hoover P et al. Cytokine induced 3-D organotypic psoriasis skin model demonstrates distinct roles for NF-kappa B and JAK pathways in disease pathophysiology Experimental dermatology 2022-02-25 [PMID: 35213752] (IF/IHC) IF/IHC
Muller C. Quantitative studies on the differentiated expression of effector molecules of the innate immune defense between extraoral skin and oral mucosa. Thesis (IHC-P, WB, Human)

Details:
Oral cavity tissues: WB, Fig 4.5; IHC(P), Fig 4.6. Psoriasin was detected below the 10 kDa marker in WB (Fig 4.5).
IHC-P, WB Human
Lundquist H, Andersson H, Chew MS et al. The Olfactomedin-4-Defined Human Neutrophil Subsets Differ in Proteomic Profile in Healthy Individuals and Patients with Septic Shock Journal of innate immunity 2022-11-30 [PMID: 36450268] (ICC/IF, Human) ICC/IF Human
Bhatt T, Bhosale A, Bajantri B et al. Sustained Secretion of the Antimicrobial Peptide S100A7 Is Dependent on the Downregulation of Caspase-8 Cell Rep 2019-11-26 [PMID: 31775025] (IHC-P, Human) IHC-P Human
Garcia M, Morello E, Garnier J et al. Pseudomonas aeruginosa flagellum is critical for invasion, cutaneous persistence and induction of inflammatory response of skin epidermis Virulence 2018-08-03 [PMID: 30070169] (IHC-P, Human) IHC-P Human
Li L, Cui Y, Ye L et al. Psoriasin overexpression confers drug resistance to cisplatin by activating ERK in gastric cancer. Int. J. Oncol. 2018-06-26 [PMID: 29956751] (IF/IHC, Human) IF/IHC Human
Bertelsen T, Iversen L, Johansen C. The human IL-17A/F heterodimer regulates psoriasis-associated genes through IkBz. Exp. Dermatol. 2018-06-25 [PMID: 29938836] (WB, Human) WB Human
Rosignoli C, Thibaut de Menonville S, Orfila D et al. A topical treatment containing heat-treated Lactobacillus johnsonii NCC 533 reduces Staphylococcus aureus adhesion and induces antimicrobial peptide expression in an in vitro reconstructed human epidermis model. Exp. Dermatol. 2018-01-30 [PMID: 29380443] (Human) Human
Doebar S C, Sieuwerts A M et al. Gene Expression Differences between Ductal Carcinoma in Situ with and without Progression to Invasive Breast Cancer. Am J Pathol 2017-01-07 [PMID: 28634007] (IF/IHC, Human) IF/IHC Human
Tian T, Li X, Hua Z et al. S100A7 promotes the migration, invasion and metastasis of human cervical cancer cells through epithelial-mesenchymal transition. Oncotarget 2017-04-11 [PMID: 28212564] (IHC-P, Human) IHC-P Human
Show All 50 Publications.

Review for S100A7/Psoriasin Antibody (NB100-56559) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56559:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
ICC Human 12/12/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23535840
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100A7/Psoriasin Antibody (NB100-56559) (0)

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Verified Customer
12/12/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol S100A7
Entrez
Uniprot