S100A7/Psoriasin Antibody (47C1068)

Images

 
Western Blot: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Psoriasin in A) MCF10A and B) MCF7 cell lysate using psoriasin antibody at 1 ug/mL.
Biological Strategies: Immunohistochemistry: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Activities of proinflammatory cytokines on the differentiation of Reconstituted Human Epidermis. RHE have been ...read more
Immunocytochemistry/ Immunofluorescence: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - S100A7/psoriasin detection was performed on (A) untreated and (B) LPS-treated (100 ng/mL, 21 hrs) HOPE-fixed NCI-H727 cells ...read more
Immunohistochemistry: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Inhibition of KDM expression in vivo. Skin biopsies from normal control skin (Cont) or lesional psoriatic skin (Pso) were collected. Skin ...read more
Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Staining of human skin. FFPE tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/mL.
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis of FFPE tissue section of human lymph node cancer using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Human breast tissue stained with psoriasin antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with ...read more
Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control ...read more
Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Image shows a specific band for S100A7/Psoriasin in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Image shows a specific band for Psoriasin/S100A7 in 0.05 mg/mL of MCF-7 lysate. This experiment was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, EM, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
47C1068
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

S100A7/Psoriasin Antibody (47C1068) Summary

Immunogen
This antibody was developed against recombinant psoriasin/HID-5 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100A7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Simple Western 1:500
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Flow (Intracellular)
Application Notes
Use in immunprecipitation reported in scientific literature (PMID 26225121). Use in Electron Microscopy reported in scientific literature (PMID: 26225121).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 4
using
NB100-56559 in the following applications:

Publications
Read Publications using
NB100-56559 in the following applications:

  • 5 publications
  • IHC
    6 publications
  • 2 publications
  • 12 publications
  • IP
    1 publication
  • WB
    13 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S100A7/Psoriasin Antibody (47C1068)

  • HID 5
  • HID5
  • HID-5
  • protein S100-A7
  • PSOR1
  • psoriasin 1
  • Psoriasin
  • S100 calcium binding protein A7 (psoriasin 1)
  • S100 calcium binding protein A7
  • S100 calcium-binding protein A7 (psoriasin 1)
  • S100 calcium-binding protein A7
  • S100A7
  • S100A7c

Background

Psoriasin/HID-5 was originally identified as an up-regulated protein in psoriatic keratinocytes (1). Subsequently, this protein was found to have elevated expression in abnormally differentiating primary keratinocytes and in various carcinomas, including ductal carcinoma in situ (2-3). Psoriasin/HID-5 may play a role in the establishment or maintenance of these pathologies, and may serve as a diagnostic marker of particular tumor types or abnormally proliferative epithelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for S100A7/Psoriasin Antibody (NB100-56559)(48)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(5)
IHC
(6)
IHC-Fr
(2)
IHC-P
(12)
IP
(1)
WB
(13)
All Applications
Filter By Species
Human
(28)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using NB100-56559 Applications Species
Bhatt T, Bhosale A, Bajantri B et al. Sustained Secretion of the Antimicrobial Peptide S100A7 Is Dependent on the Downregulation of Caspase-8 Cell Rep Nov 26 2019 [PMID: 31775025] (IHC-P, Human) IHC-P Human
Muller C. Quantitative studies on the differentiated expression of effector molecules of the innate immune defense between extraoral skin and oral mucosa. Thesis (WB, IHC-P, Human)

Details:
Oral cavity tissues: WB, Fig 4.5; IHC(P), Fig 4.6. Psoriasin was detected below the 10 kDa marker in WB (Fig 4.5).
WB, IHC-P Human
Garcia M, Morello E, Garnier J et al. Pseudomonas aeruginosa flagellum is critical for invasion, cutaneous persistence and induction of inflammatory response of skin epidermis Virulence Aug 3 2018 [PMID: 30070169] (IHC-P, Human) IHC-P Human
Li L, Cui Y, Ye L et al. Psoriasin overexpression confers drug resistance to cisplatin by activating ERK in gastric cancer. Int. J. Oncol. Jun 26 2018 [PMID: 29956751] (IHC, Human) IHC Human
Bertelsen T, Iversen L, Johansen C. The human IL-17A/F heterodimer regulates psoriasis-associated genes through IkBz. Exp. Dermatol. Jun 25 2018 [PMID: 29938836] (WB, Human) WB Human
Rosignoli C, Thibaut de Menonville S, Orfila D et al. A topical treatment containing heat-treated Lactobacillus johnsonii NCC 533 reduces Staphylococcus aureus adhesion and induces antimicrobial peptide expression in an in vitro reconstructed human epidermis model. Exp. Dermatol. 2018 Jan 30 [PMID: 29380443] (Human) Human
Doebar S C, Sieuwerts A M et al. Gene Expression Differences between Ductal Carcinoma in Situ with and without Progression to Invasive Breast Cancer. Am J Pathol Jan 7 2017 [PMID: 28634007] (IHC, Human) IHC Human
Tian T, Li X, Hua Z et al. S100A7 promotes the migration, invasion and metastasis of human cervical cancer cells through epithelial-mesenchymal transition. Oncotarget Apr 11 2017 [PMID: 28212564] (IHC-P, Human) IHC-P Human
Bertelsen T, Ljungberg C, Kjellerup RB et al. IL-17F regulates psoriasis-associated genes through IkBz. Exp. Dermatol. 2016 Aug 30 [PMID: 27576147]
Bianco K, Gormley M, Farrell J et al. Placental transcriptomes in the common aneuploidies reveal critical regions on the trisomic chromosomes and genome-wide effects Prenat. Diagn. [PMID: 27328057] (WB, ICC/IF, IHC, Human)

Details:
Citation using the Azide Free form of this antibody.
WB, ICC/IF, IHC Human
Show All 48 Publications.

Review for S100A7/Psoriasin Antibody (NB100-56559) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56559:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
ICC Human 12/12/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23535840
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23535840

Details

Detection NotesSee PMID 23535840

Comments

CommentsPublished in PMID: 23535840

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100A7/Psoriasin Antibody (NB100-56559) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56559AF350
Alexa Fluor 405 NBP2-24911AF405
Alexa Fluor 488 NBP2-24911AF488
Alexa Fluor 532 NB100-56559AF532
Alexa Fluor 594 NB100-56559AF594
Alexa Fluor 647 NBP2-24911AF647
Alexa Fluor 700 NBP2-24911AF700
Alexa Fluor 750 NB100-56559AF750
Allophycocyanin NBP2-24911APC
Biotin NBP2-24911B
DyLight 350 NBP2-24911UV
DyLight 405 NBP2-24911V
DyLight 488 NBP2-24911G
DyLight 550 NBP2-24911R
DyLight 594 NB100-56559DL594
DyLight 650 NBP2-24911C
DyLight 680 NBP2-24911FR
DyLight 755 NBP2-24911IR
FITC NBP2-24911F
HRP NBP2-24911H
Janelia Fluor 549 NB100-56559JF549
Janelia Fluor 646 NB100-56559JF646
PE NBP2-24911PE
PerCP NBP2-24911PCP

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Recent Reviews

4
5
0
4
1
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2
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1
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Anonymous
12/12/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol S100A7
Entrez
Uniprot