S100A7/Psoriasin Antibody (47C1068) - BSA Free

Western Blot: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Psoriasin in A) MCF10A and B) MCF7 cell lysate using psoriasin antibody at 1 ug/mL.

Immunohistochemistry: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Activities of proinflammatory cytokines on the differentiation of Reconstituted Human Epidermis. RHE have been cultured for 10 days at the air-water interface using an appropriate differentiation medium and then with or without recombinant IL-1alpha, IL-17A, IL-22, OSM or TN alpha alone or in combination during 72 h for immunohistological analysis. RHE were fixed, embedded in paraffin and 4 um vertical sections were stained with Hematoxylin and Eosin (HE) or with anti-CK10, anti-LOR, anti-FLG, anti-IVL or anti-S100A7 mAbs. Results are from one experiment representative of two. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0101937), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - S100A7/psoriasin detection was performed on (A) untreated and (B) LPS-treated (100 ng/mL, 21 hrs) HOPE-fixed NCI-H727 cells using the monoclonal Psoriasin/HID5/S100A7 antibody (NB100-56559). (C) Positive control is shown by the expression of the nuclear Ki67 antigen with the MIB-1 (2 ug/mL) antibody. (D) Negative control was included omitting the primary antibody.

Immunohistochemistry: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Inhibition of KDM expression in vivo. Skin biopsies from normal control skin (Cont) or lesional psoriatic skin (Pso) were collected. Skin sections were stained with Hematoxylin and immunodetection of cytokeratin 10, loricrin, filaggrin, involucrin and S100A7 was performed. Scale bar 100 um. Results are from one experiment representative of three. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0101937), licensed under a CC-BY licence.

Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.. Using the PE format of this antibody.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Staining of human skin. FFPE tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/mL.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). Diffused to granular cytoplasmic immunostaining was observed all over the tissue section with highest expression levels in the areas with cancer cells especially in the developing squamous keratin pearls.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis of FFPE tissue section of human lymph node cancer using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). Very strong cytoplasmic immunopositivity of Psoriasin/S100A7 protein was observed in the cancer cells.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Human breast tissue stained with psoriasin antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with citrate buffer, pH 6.

Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Image shows a specific band for S100A7/Psoriasin in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Image shows a specific band for Psoriasin/S100A7 in 0.05 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, EM, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: 47C1068
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

S100A7/Psoriasin Antibody (47C1068) - BSA Free Summary

• Immunogen: This antibody was developed against recombinant psoriasin/HID-5 protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100A7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Flow (Intracellular)
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western 1:500
  • Western Blot 1-3 ug/ml
• Application Notes: Use in immunprecipitation reported in scientific literature (PMID 26225121). Use in Electron Microscopy reported in scientific literature (PMID: 26225121).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:25622979).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for S100A7/Psoriasin Antibody (47C1068) - BSA Free

• HID 5
• HID5
• HID-5
• protein S100-A7
• PSOR1
• psoriasin 1
• Psoriasin
• S100 calcium binding protein A7 (psoriasin 1)
• S100 calcium binding protein A7
• S100 calcium-binding protein A7 (psoriasin 1)
• S100 calcium-binding protein A7
• S100A7
• S100A7c

Background

Psoriasin/HID-5 was originally identified as an up-regulated protein in psoriatic keratinocytes (1). Subsequently, this protein was found to have elevated expression in abnormally differentiating primary keratinocytes and in various carcinomas, including ductal carcinoma in situ (2-3). Psoriasin/HID-5 may play a role in the establishment or maintenance of these pathologies, and may serve as a diagnostic marker of particular tumor types or abnormally proliferative epithelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A7/Psoriasin Antibody (NB100-56559)(49)

Publications using NB100-56559

• Todorovic, McDonald HA, Hoover P et al. Cytokine induced 3-D organotypic psoriasis skin model demonstrates distinct roles for NF-kappa B and JAK pathways in disease pathophysiology Experimental dermatology 2022-02-25 [PMID: 35213752] (IHC)
• Muller C. Quantitative studies on the differentiated expression of effector molecules of the innate immune defense between extraoral skin and oral mucosa. Thesis (IHC-P, WB, Human)
  
  Details:
  Oral cavity tissues: WB, Fig 4.5; IHC(P), Fig 4.6. Psoriasin was detected below the 10 kDa marker in WB (Fig 4.5).
• Bhatt T, Bhosale A, Bajantri B et al. Sustained Secretion of the Antimicrobial Peptide S100A7 Is Dependent on the Downregulation of Caspase-8 Cell Rep 2019-11-26 [PMID: 31775025] (IHC-P, Human)
• Garcia M, Morello E, Garnier J et al. Pseudomonas aeruginosa flagellum is critical for invasion, cutaneous persistence and induction of inflammatory response of skin epidermis Virulence 2018-08-03 [PMID: 30070169] (IHC-P, Human)
• Li L, Cui Y, Ye L et al. Psoriasin overexpression confers drug resistance to cisplatin by activating ERK in gastric cancer. Int. J. Oncol. 2018-06-26 [PMID: 29956751] (IHC, Human)
• Bertelsen T, Iversen L, Johansen C. The human IL-17A/F heterodimer regulates psoriasis-associated genes through IkBz. Exp. Dermatol. 2018-06-25 [PMID: 29938836] (WB, Human)
• Rosignoli C, Thibaut de Menonville S, Orfila D et al. A topical treatment containing heat-treated Lactobacillus johnsonii NCC 533 reduces Staphylococcus aureus adhesion and induces antimicrobial peptide expression in an in vitro reconstructed human epidermis model. Exp. Dermatol. 2018-01-30 [PMID: 29380443] (Human)
• Doebar S C, Sieuwerts A M et al. Gene Expression Differences between Ductal Carcinoma in Situ with and without Progression to Invasive Breast Cancer. Am J Pathol 2017-01-07 [PMID: 28634007] (IHC, Human)
• Tian T, Li X, Hua Z et al. S100A7 promotes the migration, invasion and metastasis of human cervical cancer cells through epithelial-mesenchymal transition. Oncotarget 2017-04-11 [PMID: 28212564] (IHC-P, Human)
• Bertelsen T, Ljungberg C, Kjellerup RB et al. IL-17F regulates psoriasis-associated genes through IkBz. Exp. Dermatol. 2016-08-30 [PMID: 27576147]
• Bianco K, Gormley M, Farrell J et al. Placental transcriptomes in the common aneuploidies reveal critical regions on the trisomic chromosomes and genome-wide effects Prenat. Diagn. [PMID: 27328057] (ICC/IF, IHC, WB, Human)
  
  Details:
  Citation using the Azide Free form of this antibody.
• Koerdt S, Steinstraesser L, Stoeckelhuber M. Radiotherapy for oral cancer decreases the cutaneous expression of host defence peptides. J Craniomaxillofac Surg. 2016-04-22 [PMID: 27193476] (IHC-P, Human)
• Chu YW, Liu ST, Cheng HC et al. Opposing Effects of Zac1 and Curcumin on AP-1-Regulated Expressions of S100A7. PLoS ONE. 2015-12-04 [PMID: 26633653] (WB, Human)
  
  Details:
  S100A7/Psoriasin antibody (clone 47C1068) was used for WB assays in experiments involving HaCaT/keratinocyte cells which were treated or not with curcumin (Fig 1C).
• Dey KK, Sarkar S, Pal I et al. Mechanistic attributes of S100A7 (psoriasin) in resistance of anoikis resulting tumor progression in squamous cell carcinoma of the oral cavity. Cancer Cell Int. 2015-07-30 [PMID: 26225121] (ICC/IF, IHC-P, IP, WB, Human)
  
  Details:
  S100A7/Psoriasin antibody was used for WB, IP and IHC-P assays in experiments involving orthotopic tumors model and in-vitro cultures of TU167, JMAR, JMARC39, JMARC42 and MDA-MB-468 human cancer cell lines. WB was performed on lysates of attached/detached assay cell lysates of TU167 and JMAR cells (1A, 3a & b), and saliva as well as norma or tumorous tissues from human head and neck cancer patients (Fig. 2B, 3b & c). WB was also performed on lysates of JMAR cells transfected with S100A7 - siRNA (Fig. 4) as well as lysates of TU167 cells with vector and S100A7 expression stable clones (Fig. 5A). S100A7 IP was performed on conditioned medium from TU167 and JMAR cells (Fig 3B). ICC-IF was performed on JMAR detached cells (Fig 1b) as well as TU167 cells with vector and S100A7 expression stable clones (Fig 5c). IHC-P was performed on tumor sections of mice subjected to orthotopic injection of TU167 and JMAR cell lines (Fig 1e) or TU167 cells with vector and S100A7 expression stable clones (Fig. 6c), and on tissues from normal or head and neck cancer patients (Fig 2a).
• Zhao H, Wilkie T, Deol Y et al. miR-29b defines the pro-/anti-proliferative effects of S100A7 in breast cancer Mol. Cancer 2015-01-27 [PMID: 25622979] (IHC, Mouse)
• de Castro Alice, Minty Fay, Hattinger Eva et al. The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators. Longev Healthspan. 2014-01-01 [PMID: 25621169] (WB, Human)
• Mcheik JN, Barrault C, Pedretti N et al. Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children. Burns. 2014-09-15 [PMID: 25234956] (IHC, Human)
• Rabeony Hanitriniaina, Petit-Paris Isabelle, Garnier Julien et al. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS One. 2014-01-01 [PMID: 25010647] (IHC)
• Teng X, Hu Z, Wei X et al. IL-37 Ameliorates the Inflammatory Process in Psoriasis by Suppressing Proinflammatory Cytokine Production. J. Immunol. 2014-02-15 [PMID: 24453242] (WB, Human)
  
  Details:
  HaCaT immortalized human keratinocytes, Fig 1D.
• Zhu L, Okano S, Takahara M et al. Expression of S100 protein family members in normal skin and sweat gland tumors. J Dermatol Sci. 2013-06-01 [PMID: 23623205]
  
  Details:
  Psoriasin/HID5/S100A7 Clone 47C1068 (IMG-409A), IHC (paraffin): Normal human skin and sweat gland tumors, Figs 1k-O & 5A-G, Tables 1-3. Note: The 47C1068 S100A7 mAb was used at 10 ug/ml.
• Sneh A, Deol YS, Ganju A et al. Differential role of psoriasin (S100A7) in estrogen receptor a positive and negative breast cancer cells occur through actin remodeling Breast Cancer Res Treat. 2013-04-01 [PMID: 23535840] (ICC/IF, Human)
• McHeik JN, Barrault C, Pedretti N et al. Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts. J Tissue Eng Regen Med. 2013-03-14 [PMID: 23495214]
• Vegfors J, Petersson S, Kovacs A et al. The Expression of Psoriasin (S100A7) and CD24 Is Linked and Related to the Differentiation of Mammary Epithelial Cells. PLoS One 2012-12-27 [PMID: 23300877]
• Ye L, Sun PH, Martin TA et al. Psoriasin (S100A7) is a positive regulator of survival and invasion of prostate cancer cells. Urol Oncol 2013-11-01 [PMID: 22694938] (IHC-Fr, Human)
  
  Details:
  Prostate cancer and normal prostate tissue, Fig 1 A-F.
• Shubbar E, Vegfors J, Carlstrom M et al. Psoriasin (S100A7) increases the expression of ROS and VEGF and acts through RAGE to promote endothelial cell proliferation. Breast Cancer Res Treat 2011-12-22 [PMID: 22189627]
• Nofech-Mozes S, Spayne J, Rakovitch E et al. Biological Markers Predictive of Invasive Recurrence in DCIS. Clin Med Oncol. 2008-01-01 [PMID: 21892261] (IHC-P, Human)
  
  Details:
  IHC (Paraffin), human breast tissue, Fig. 2E, Table 1.
• Kesting MR, Mueller C, Wagenpfeil S et al. Quantitative comparison of the expression of antimicrobial peptides in the oral mucosa and extraoral skin. Br J Oral Maxillofac Surg. 2012-07-01 [PMID: 21820218]
• Garreis F, Gottschalt M, Schlorf T et al. Expression and regulation of antimicrobial peptide psoriasin (S100A7) at the ocular surface and in the lacrimal apparatus. Invest Ophthalmol Vis Sci. 2011-07-01 [PMID: 21551409] (IHC-P, WB)
  
  Details:
  Psoriasin/HID5/S100A7 (IMG-409A). IHC (paraffin) & WB: Human corneal epithelial (HCE), conjunctivial epithelial (HCjE), lacrimal glands (lg), nasolacrimal ducts (nd), conjunctivae (cj), corneae cells, Fig 1 & 4.
• Andresen E, Lange C, Strodthoff D et al. S100A7/psoriasin expression in the human lung: unchanged in patients with COPD, but upregulated upon positive S. aureus detection. BMC Pulm Med. 2011-02-15 [PMID: 21324122] (ICC/IF)
  
  Details:
  Psoriasin/HID5/S100A7 (IMG-409A). IF/ICC: Untreated or LPS treated bronchial epithelial cell line (NCI-H727), Fig 3.
• Guilloteau K, Paris I, Pedretti N et al. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1{alpha}, and TNF-{alpha} Recapitulates Some Features of Psoriasis. J Immunol. 2010-03-24 [PMID: 20335534]
• Hashiramoto A, Yamane T, Tsumiyama K et al. Mammalian clock gene Cryptochrome regulates arthritis via proinflammatory cytokine TNF-alpha. J Immunol. 2010-02-01 [PMID: 20042581] (WB, Human)
  
  Details:
  S100A7 (IMG-409A): WB, human neutrophils, Fig 6.
• Kesting Marco Rainer, Sudhoff Holger, Hasler Rafael Johannes et al. Psoriasin (S100A7) up-regulation in oral squamous cell carcinoma and its relation to clinicopathologic features. Oral Oncol. 2009-08-01 [PMID: 19147391] (Human)
• Wolf R, Voscopoulos C, Winston J et al. Highly homologous hS100A15 and hS100A7 proteins are distinctly expressed in normal breast tissue and breast cancer. Cancer Lett. 2009-05-08 [PMID: 19136201] (WB, Human)
  
  Details:
  WB (rhS100A7 and rhS100A15), Fig. 1b IHC-F (human normal adult breast tissue), Fig. 2b WB (human normal breast tissue and invasive breast carcinoma), Fig. 3a IHC-F (human normal and invasive breast carcinoma), Fig. 3b WB (human invasive ductal carcinomas)
• Wolf R, Howard OM, Dong HF et al. Chemotactic Activity of S100A7 (Psoriasin) Is Mediated by the Receptor for Advanced Glycation End Products and Potentiates Inflammation with Highly Homologous but Functionally Distinct S100A15. J Immunol 2008-07-15 [PMID: 18606705] (IHC-Fr, WB, Human)
• Pene J, Chevalier S, Preisser L et al. Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes. J Immunol. 2008-06-01 [PMID: 18490742] (ICC/IF, Human)
  
  Details:
  Psoriasin/HID5/S100A7 (IMG-409A). IF/ICC: Reconstituted human epidermis, Fig 5.
• Zhang H, Zhao Q, Chen Y et al. Selective expression of S100A7 in lung squamous cell carcinomas and large cell carcinomas but not in adenocarcinomas and small cell carcinomas. Thorax. 2008-04-01 [PMID: 18364444] (Human)
• Paruchuri V, Prasad A, McHugh K et al. S100A7-downregulation inhibits epidermal growth factor-induced signaling in breast cancer cells and blocks osteoclast formation. PLoS One. 2008-03-05 [PMID: 18320059] (WB, Human)
  
  Details:
  WB (human breast cancer cell lines), Figs. 1A (MCF-7 cells), B (MDA-MB-468 cells); 2A (MDA-MB-468 cells).
• Petersson S, Bylander A, Yhr M et al. S100A7 (Psoriasin), highly expressed in ductal carcinoma in situ (DCIS), is regulated by IFN-gamma in mammary epithelial cells. BMC Cancer. 2007-11-06 [PMID: 17986321]
  
  Details:
  WB: Fig 1 (MCF10A, HEKn, and MDA-MB-468 cells), Fig 3 (MDA-MB-468, HEKn, MCF10A, and SUM190 cells), Fig 5 (MCF10A cells), and Fig 6 (TAC2-tetEGFP-2-psoriasin cells). The HID5/Psoriasin/S100A7 (IMG-409) mAb was transfected validated by WB using a tetracyli
• Zhang H, Wang Y, Chen Y et al. Identification and validation of S100A7 associated with lung squamous cell carcinoma metastasis to brain. Lung Cancer. 2007-07-01 [PMID: 17418446]
• Boniface K, Diveu C, Morel F et al. Oncostatin M secreted by skin infiltrating T lymphocytes is a potent keratinocyte activator involved in skin inflammation. J Immunol. 2007-04-01 [PMID: 17372020] (IHC-P, WB, Human)
• Sa SM, Valdez PA, Wu J et al. The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis. J Immunol. 2007-02-15 [PMID: 17277128] (IHC-P, Human)
  
  Details:
  (IHC-formalin-fixed, paraffin-embedded: human epidermis model, Fig. 3C)
• Mehul B, Bernard D, Brouard M et al. Influence of calcium on the proteolytic degradation of the calmodulin-like skin protein (calmodulin-like protein 5) in psoriatic epidermis. Exp Dermatol. 2006-06-01 [PMID: 16689864]
• Krop I, Marz A, Carlsson H et al. A putative role for psoriasin in breast tumor progression. Cancer Res. 2005-12-15 [PMID: 16357139] (IHC-P)
• Kennedy RD, Gorski JJ, Quinn JE et al. BRCA1 and c-Myc associate to transcriptionally repress psoriasin, a DNA damage-inducible gene. Cancer Res. 2005-11-15 [PMID: 16288014]
• Boniface K, Bernard FX, Garcia M et al. IL-22 inhibits epidermal differentiation and induces proinflammatory gene expression and migration of human keratinocytes. J Immunol. 2005-03-15 [PMID: 15749908]
• Martinsson H, Yhr M, Enerback C. Expression patterns of S100A7 (psoriasin) and S100A9 (calgranulin-B) in keratinocyte differentiation. Exp Dermatol. 2005-03-01 [PMID: 15740587]
• Glaser R, Harder J, Lange H et al. Antimicrobial psoriasin (S100A7) protects human skin from Escherichia coli infection. Nat Immunol. 2005-01-01 [PMID: 15568027] (IHC-P, Human)
  
  Details:
  Psoriasin/HID5/S100A7 (IMG-409A). IHC (paraffin): Human epithelial tissue: nose skin, lower leg epidermis, cheek epidermis, hair follicle, Fig 3.
• Porter D, Lahti-Domenici J, Keshaviah A et al. Molecular markers in ductal carcinoma in situ of the breast. Mol Cancer Res. 2003-03-01 [PMID: 12651909]
  
  Details:
  IHC(paraffin), Fig 2A,C.
• Enerback C, Porter DA, Seth P et al. Psoriasin expression in mammary epithelial cells in vitro and in vivo. Cancer Res. 2002-01-01 [PMID: 11782356]

Review for S100A7/Psoriasin Antibody (NB100-56559) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: cynthia morimoto ICC Human 12/12/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23535840
• Species: Human

Bioinformatics

• Gene Symbol: S100A7
• Entrez:
  • 6278(Human)
• Uniprot:
  • P31151()