S100A7/Psoriasin Antibody (47C1068)

Images

 
Western Blot: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Psoriasin in A) MCF10A and B) MCF7 cell lysate using psoriasin antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Psoriasin/HID5/S100A7 immunocytochemical detection was performed on (A) untreated and (B) LPS-treated (100 ng/ml, 21 hrs) ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Formalin-fixed, paraffin-embedded human breast tissue stained with psoriasin antibody ...read more
Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Staining of human skin. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/ml ...read more
Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis of formalin-fixed paraffin-embedded tissue section of human lymph node cancer using 5 ug/ml concentration of Psoriasin/S100A7 ...read more
Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Simple Western lane view shows a specific band for S100A7/Psoriasin in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing ...read more
Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Simple Western lane view shows a specific band for Psoriasin/S100A7 in 0.05 mg/ml of MCF-7 lysate. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
47C1068
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

S100A7/Psoriasin Antibody (47C1068) Summary

Immunogen
This antibody was developed against recombinant psoriasin/HID-5 protein.
Specificity
In confluent MCF10A cells, an 11 kDa band is observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100A7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Flow (Intracellular)
Application Notes
Use in immunprecipitation reported in scientific literature (PMID 26225121).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 4
using
NB100-56559 in the following applications:

Publications
Read Publications using
NB100-56559 in the following applications:

  • 5 publications
  • IHC
    3 publications
  • 2 publications
  • 10 publications
  • IP
    1 publication
  • WB
    13 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S100A7/Psoriasin Antibody (47C1068)

  • HID 5
  • HID5
  • HID-5
  • protein S100-A7
  • PSOR1
  • psoriasin 1
  • Psoriasin
  • S100 calcium binding protein A7 (psoriasin 1)
  • S100 calcium binding protein A7
  • S100 calcium-binding protein A7 (psoriasin 1)
  • S100 calcium-binding protein A7
  • S100A7
  • S100A7c

Background

Psoriasin/HID-5 was originally identified as an up-regulated protein in psoriatic keratinocytes (1). Subsequently, this protein was found to have elevated expression in abnormally differentiating primary keratinocytes and in various carcinomas, including ductal carcinoma in situ (2-3). Psoriasin/HID-5 may play a role in the establishment or maintenance of these pathologies, and may serve as a diagnostic marker of particular tumor types or abnormally proliferative epithelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for S100A7/Psoriasin Antibody (NB100-56559)(43)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(5)
IHC
(3)
IHC-Fr
(2)
IHC-P
(10)
IP
(1)
WB
(13)
All Applications
Filter By Species
Human
(24)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using NB100-56559 Applications Species
Tian T, Li X, Hua Z et al. S100A7 promotes the migration, invasion and metastasis of human cervical cancer cells through epithelial-mesenchymal transition. Oncotarget Apr 11 2017 [PMID: 28212564] (IHC-P, Human) IHC-P Human
Bertelsen T, Ljungberg C, Kjellerup RB et al. IL-17F regulates psoriasis-associated genes through IkBz. Exp. Dermatol. 2016 Aug 30 [PMID: 27576147]
Bianco K, Gormley M, Farrell J et al. Placental transcriptomes in the common aneuploidies reveal critical regions on the trisomic chromosomes and genome-wide effects Prenat. Diagn. [PMID: 27328057] (WB, ICC/IF, IHC, Human)

Details:
Citation using the Azide Free form of this antibody.
WB, ICC/IF, IHC Human
Koerdt S, Steinstraesser L, Stoeckelhuber M. Radiotherapy for oral cancer decreases the cutaneous expression of host defence peptides. Journal of Cranio-Maxillofacial Surgery. 2016 Apr 22 [PMID: 27193476] (IHC-P, Human) IHC-P Human
Chu YW, Liu ST, Cheng HC et al. Opposing Effects of Zac1 and Curcumin on AP-1-Regulated Expressions of S100A7. PLoS ONE. 2015 Dec 04 [PMID: 26633653] (WB, Human)

Details:
S100A7/Psoriasin antibody (clone 47C1068) was used for WB assays in experiments involving HaCaT/keratinocyte cells which were treated or not with curcumin (Fig 1C).
WB Human
Dey KK, Sarkar S, Pal I et al. Mechanistic attributes of S100A7 (psoriasin) in resistance of anoikis resulting tumor progression in squamous cell carcinoma of the oral cavity. Cancer Cell Int. 2015 Jul 30 [PMID: 26225121] (WB, IP, IHC-P, ICC/IF, Human)

Details:
S100A7/Psoriasin antibody was used for WB, IP and IHC-P assays in experiments involving orthotopic tumors model and in-vitro cultures of TU167, JMAR, JMARC39, JMARC42 and MDA-MB-468 human cancer cell lines. WB was performed on lysates of attached/detached assay cell lysates of TU167 and JMAR cells (1A, 3a & b), and saliva as well as norma or tumorous tissues from human head and neck cancer patients (Fig. 2B, 3b & c). WB was also performed on lysates of JMAR cells transfected with S100A7 - siRNA (Fig. 4) as well as lysates of TU167 cells with vector and S100A7 expression stable clones (Fig. 5A). S100A7 IP was performed on conditioned medium from TU167 and JMAR cells (Fig 3B). ICC-IF was performed on JMAR detached cells (Fig 1b) as well as TU167 cells with vector and S100A7 expression stable clones (Fig 5c). IHC-P was performed on tumor sections of mice subjected to orthotopic injection of TU167 and JMAR cell lines (Fig 1e) or TU167 cells with vector and S100A7 expression stable clones (Fig. 6c), and on tissues from normal or head and neck cancer patients (Fig 2a).
WB, IP, IHC-P, ICC/IF Human
Zhao H, Wilkie T, Deol Y et al. miR-29b defines the pro-/anti-proliferative effects of S100A7 in breast cancer Mol. Cancer. 2015 Jan 27 [PMID: 25622979] (Human) Human
de Castro Alice, Minty Fay, Hattinger Eva et al. The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators. Longev Healthspan. 2014 [PMID: 25621169] (WB, Human) WB Human
Mcheik JN, Barrault C, Pedretti N et al. Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children. Burns. 2014 Sep 15 [PMID: 25234956] (IHC, Human)

Details:
Psoriasin/S100A7 antibody used for IHC - In situ labelling on epidermal tissue reconstituted from human keratinocytes isolated from foreskin, auricular skin, chest and abdominal skin (Fig. 1 )
IHC Human
Rabeony Hanitriniaina, Petit-Paris Isabelle, Garnier Julien et al. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS One. 2014 [PMID: 25010647] (IHC) IHC
Show All 43 Publications.

Review for S100A7/Psoriasin Antibody (NB100-56559) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NB100-56559:
Filter by Applications
ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Human 12/12/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23535840
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23535840

Details

Detection NotesSee PMID 23535840

Comments

CommentsPublished in PMID: 23535840

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S100A7/Psoriasin Antibody (NB100-56559) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-24911APC
Biotin Labeled NBP2-24911B
DyLight 350 Labeled NBP2-24911UV
DyLight 405 Labeled NBP2-24911V
DyLight 488 Labeled NBP2-24911G
DyLight 550 Labeled NBP2-24911R
DyLight 650 Labeled NBP2-24911C
DyLight 680 Labeled NBP2-24911FR
DyLight 755 Labeled NBP2-24911IR
HRP Labeled NBP2-24911H
PE Labeled NBP2-24911PE

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Recent Reviews

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Anonymous
12/12/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol S100A7
Entrez
Uniprot