Osterix/Sp7 Antibody (764704)

Images

 
Western blot shows lysates of Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse ...read more
Osterix/Sp7 was detected in immersion fixed Saos‑2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
764704
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Osterix/Sp7 Antibody (764704) Summary

Immunogen
E. coli-derived recombinant human Osterix/Sp7
Met19-Leu288
Accession # Q8TDD2
Specificity
Detects human Osterix/Sp7 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SP7
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osterix/Sp7 Antibody (764704)

  • MGC126598
  • Osterix
  • OSX
  • OSXosterix
  • Sp7 transcription factor
  • Sp7
  • transcription factor Sp7
  • Zinc finger protein osterix

Background

Osterix, also known as Sp7, is an approximately 45 kDa transcription factor that is required for osteogenesis and bone homeostasis. Osterix cooperates with BMP-6 in the differentiation of osteoblasts from mesenchymal stem cells by regulating the transcription of several genes involved in osteoblast differentiation and function (i.e. SATB2, Collagen V, and SOST). The transcription of Osterix is induced by BMP-2, IGF-I, and parathyroid hormone, while its activity is regulated by Akt and p38 MAPK-mediated phoshorylation. Osterix contains a transactivation domain (aa 141-210) and three zinc finger domains (aa 294-318, aa 324-348, and aa 354-376). Alternative splicing generates a short isoform that lacks the N-terminal 18 amino acids. Within aa 19-288, human Osterix shares 94% aa sequence identity with mouse and rat Osterix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-77461
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
355-BM/CF
Species: Hu, Mu, Rt
NB110-3638
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NBP1-89131
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-42378
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-32263
Species: Hu
Applications: WB, IHC, IP
NBP1-89134
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
355-BM/CF
Species: Hu, Mu, Rt
NB100-56505
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
NBP1-85793
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-85445
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for Osterix/Sp7 Antibody (MAB7547) (0)

There are no publications for Osterix/Sp7 Antibody (MAB7547).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Osterix/Sp7 Antibody (MAB7547) (0)

There are no reviews for Osterix/Sp7 Antibody (MAB7547). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Osterix/Sp7 Antibody (MAB7547) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Osterix/Sp7 Products

Bioinformatics Tool for Osterix/Sp7 Antibody (MAB7547)

Discover related pathways, diseases and genes to Osterix/Sp7 Antibody (MAB7547). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Osterix/Sp7 Antibody (MAB7547)

Discover more about diseases related to Osterix/Sp7 Antibody (MAB7547).
 

Pathways for Osterix/Sp7 Antibody (MAB7547)

View related products by pathway.

PTMs for Osterix/Sp7 Antibody (MAB7547)

Learn more about PTMs related to Osterix/Sp7 Antibody (MAB7547).

Blogs on Osterix/Sp7

There are no specific blogs for Osterix/Sp7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Osterix/Sp7 Antibody (764704) and receive a gift card or discount.

Bioinformatics

Gene Symbol SP7
Entrez
Uniprot