RRM1 Antibody

Western Blot: RRM1 Antibody [NBP2-20231] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: RRM1 Antibody [NBP2-20231] - Green: RRM1 protein stained by RRM1 antibody. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: RRM1 Antibody [NBP2-20231] - Gastric cancer. RRM1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: RRM1 Antibody [NBP2-20231] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug Raw264. 7 whole cell lysate/extract C. 30 ug C2C12 whole cell lysate/extract5 % SDS-PAGE RRM1 antibody dilution: 1:1000.

Immunohistochemistry-Paraffin: RRM1 Antibody [NBP2-20231] - Human colon carcinoma, using RRM1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RRM1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RRM1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%), Xenopus laevis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for RRM1 Antibody

• EC 1.17.4.1
• R1
• ribonucleoside-diphosphate reductase large subunit
• Ribonucleoside-diphosphate reductase subunit M1
• Ribonucleotide reductase large subunit
• ribonucleotide reductase M1 polypeptide
• ribonucleotide reductase M1
• ribonucleotide reductase, large subunit
• ribonucleotide reductase, R1 subunit
• RIR1
• RR1
• RRM1

Background

This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RRM1
• Uniprot:
  • P23921(Human)