IP3R1 Antibody

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

IP3R1 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues N(1829) K K K D D E V D R D A P S R K K A K E(1848) of human IP3 Receptor Type I.
Localization
Endoplasmic reticulum - Integral membrane protein.
Specificity
IP3R1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITPR1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
WB: Detects an approx. 240 - 260 kDa protein representing IP3R-I from rat and mouse brain extract.
Reviewed Applications
Read 1 Review rated 5
using
NB120-5908 in the following applications:

Publications
Read Publications using
NB120-5908 in the following applications:

Reactivity Notes

Human reactivity reported by a customer review. Mouse reactivity reported in scientific literature (PMID: 19382908). Rat reactivity reported in scientific literature (PMID: 12767046).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole serum diluted in PBS
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for IP3R1 Antibody

  • DKFZp313E1334
  • DKFZp313N1434
  • inositol 14,5-triphosphate receptor, type 1
  • inositol 14,5-trisphosphate receptor type 1
  • INSP3R1
  • IP3 receptor isoform 1
  • IP3 receptor
  • IP3R 1
  • IP3R
  • IP3R1
  • SCA15
  • SCA16
  • spinocerebellar ataxia 15
  • spinocerebellar ataxia 16
  • Type 1 inositol 14,5-trisphosphate receptor
  • Type 1 InsP3 receptor

Background

Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to the IP3 receptor (IP3R), IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240 kDa glycoprotein subunits, that is structurally reminiscent of members of the voltage-gated ion channel superfamily. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Cytosolic calcium levels appear to be regulated by a feedback loop that starts with calcium activation of protein kinase C (PKC) and calcineurin, a protein phosphatase. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. Type-I IP3R has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II protein is found predominantly in the brain. Isoform III expression has been shown to occur in pancreatic islets, kidney and the gastrointestinal tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-5908
Species: Hu, Mu, Rt
Applications: WB, ICC/IF

Publications for IP3R1 Antibody (NB120-5908)(3)

Review for IP3R1 Antibody (NB120-5908) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB120-5908:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot IP3R1 NB120-5908
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5
reviewed by:
Verified Customer
WB Human 11/17/2010
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Summary

ApplicationWestern Blot
Sample TestedJurkat whole cell lysate, primary CLL cells from human blood, Sample Amount: 80ug
SpeciesHuman
Comments1:1000 antibody dilution80 ug of proteintransferred to PVDF for 2 hrs. at 250mAregular ECL reagent (not plus)good signal within 1 minute of exposure to film

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk in TBS-T, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit HRP, Secondary Ab Dilution Ratio: 1:10,000
Secondary Manufacturer Cat#Amersham NA9341ML

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 min, Specific Bands: above 250kDa, Non-Specific Bands: very little, below expected size

Comments

Comments1:1000 antibody dilution80 ug of proteintransferred to PVDF for 2 hrs. at 250mAregular ECL reagent (not plus)good signal within 1 minute of exposure to film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IP3R1 Antibody (NB120-5908) (0)

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Secondary Antibodies

 

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Array NB120-5908

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Recent Reviews

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5
1
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1
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Verified Customer
11/17/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol ITPR1
Entrez
Uniprot