RRM2 Antibody - Azide Free

Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution. Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Rat intestine. RRM2 antibody [N1C1] iluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: RRM2 Antibody [NBP1-31661] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] diluted at 1:1000.

Immunohistochemistry: RRM2 Antibody [NBP1-31661] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RRM2 antibody [N1C1] (NBP1-31661) at 1:300 dilution.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. RRM2 antibody [N1C1] (NBP1-31661) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] (NBP1-31661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Ze, Bv
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

RRM2 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit RRM2 Antibody - Azide Free (NBP1-31661) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-RRM2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RRM2. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRM2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western 1:25 - 1:400
  • Western Blot Assay dependent
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in ANBL6 Whole cell lysate, separated by Size, antibody dilution of 1:25,1:100,1:400, apparent MW was 45 kDa
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RRM2 Antibody - Azide Free

• C2orf48
• EC 1.17.4.1
• FLJ25102
• R2
• ribonucleoside-diphosphate reductase subunit M2
• ribonucleotide reductase M2 polypeptide
• ribonucleotide reductase M2
• Ribonucleotide reductase small chain
• Ribonucleotide reductase small subunit
• RR2
• RR2M
• RRM2

Background

Ribonucleotide reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. It is composed of 2 non-identical subunits, proteins M1 and M2. Synthesis of M2 is regulated in a cell-cycle dependent fashion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RRM2 Antibody (NBP1-31661)(5)

Publications using NBP1-31661

• MacMillan A, Karki B, Yang J et al. PRPS activity tunes redox homeostasis in Myc-driven lymphoma Redox Biology 2025-07-01 [PMID: 40446642]
• Abdel-Rahman MA, Mahfouz M, Habashy HO RRM2 expression in different molecular subtypes of breast cancer and its prognostic significance Diagnostic pathology 2022-01-05 [PMID: 34986845] (IHC-P, Human)
• White JR, Thompson DT, Koch KE et al. AP-2?-Mediated Activation of E2F and EZH2 Drives Melanoma Metastasis Cancer Research 2021-09-01 [PMID: 34210752] (Western Blot, Human)
• Chen YH, Chen YC, Lin CC et al. Synergistic Anticancer Effects of Gemcitabine with Pitavastatin on Pancreatic Cancer Cell Line MIA PaCa-2 in vitro and in vivo Cancer Manag Res 2020-06-17 [PMID: 32606957] (Western Blot, Human)
• Leslie PL, Chao YL, Tsai YH et al. Histone deacetylase 11 inhibition promotes breast cancer metastasis from lymph nodes Nat Commun 2019-09-13 [PMID: 31519896] (WB, Human)

Review for RRM2 Antibody (NBP1-31661) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 08/11/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: formalin fixed paraffin embedded mouse tumor tissues
• Species: Mouse
• Lot: 42151

FAQs for RRM2 Antibody (NBP1-31661). (Showing 1 - 1 of 1 FAQs).

1. What is the isotype of this antibody?
   • NBP1-31661 is a polyclonal antibody made in rabbit. It is expected that the majority of the antibodies present will be IgG.

Bioinformatics

• Gene Symbol: RRM2
• Entrez:
  • 6241(Human)
• Uniprot:
  • P31350(Human)