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CD1e Antibody (704407) [Unconjugated]

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Jurkat human acute T cell leukemia cell line was treated for 3 hours with monensin, then stained with Mouse Anti-Human CD1e Monoclonal Antibody (Catalog # MAB7330, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Jurkat human acute T cell leukemia cell line was treated for 3 hours with monensin, then stained with Mouse Anti-Human CD1e Monoclonal Antibody (Catalog # MAB7330, filled histogram) or isotype control antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow
Clone
704407
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC7330U-100UG
Alexa Fluor 405
IC7330V-100UG
Alexa Fluor 488
IC7330G-100UG
Alexa Fluor 532
FAB7330X
Alexa Fluor 594
IC7330T-100UG
Alexa Fluor 647
IC7330R-100UG
Alexa Fluor 700
IC7330N-100UG
Alexa Fluor 750
IC7330S-100UG
Allophycocyanin
FAB7330A
Biotin
FAB7330B
DyLight 350
FAB7330D
DyLight 405
FAB7330E
DyLight 488
FAB7330K
DyLight 550
FAB7330L
DyLight 594
FAB7330M
DyLight 650
FAB7330W
DyLight 680
FAB7330Y
DyLight 755
FAB7330Z
FITC
FAB7330F
Janelia Fluor 549
FAB7330I
Janelia Fluor 646
FAB7330J
PE
FAB7330P
PerCP
FAB7330C
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7330-SP
Carrier Free
IC7330R-100UG
Carrier Free
IC7330N-100UG
Carrier Free
IC7330G-100UG
Carrier Free
IC7330V-100UG
Carrier Free
IC7330U-100UG
Carrier Free
IC7330T-100UG
Carrier Free
IC7330S-100UG
100% Guarantee on every product

CD1e Antibody (704407) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD1e
Met1-Tyr304
Accession # P15812
Specificity
Detects human CD1e in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1d, or recombinant rat beta 2-Microglobulin is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD1E
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD1e Antibody (704407) [Unconjugated]

  • CD1-alpha-3
  • CD1e molecule
  • CD1e
  • CDIEL
  • e polypeptide
  • FLJ17609
  • membrane-associated
  • R2G1
  • thymocyte antigen CD1E

Background

CD1e is an approximately 40 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1e associates with beta 2-microglobulin in endosomes and the Golgi where it facilitates the processing of glycolipids for their presentation by other CD1 family proteins. CD1e is expressed by most nonhuman mammals but not by mice or rats. It contains one Ig-like domain in its extracellular region. Alternate splicing of human CD1e generates multiple isoforms with various deletions and substitutions in the extracellular and/or cytoplasmic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD1E
Uniprot