RPTOR Antibody - BSA Free

Western Blot: RPTOR Antibody [NBP1-77211] - Analysis of RAPTOR expression in Jurkat cell lysate with RAPTOR antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211] - Immunofluorescence of RPTOR in L1210 cells with RPTOR antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211] - Immunocytochemistry of RAPTOR in L1210 cells with RAPTOR antibody at 10 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RPTOR Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the amino-terminus of human Raptor. The immunogen is located within amino acids 90 - 140 of Raptor. Amino Acid Squence: KQYENWQPRARYK
• Specificity: Raptor has multiple isoforms that may also be recognized by antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPTOR
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 2-4 ug/ml
• Control Peptide:

  RPTOR Peptide (NBP1-77211PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RPTOR Antibody - BSA Free

• KIAA1303
• KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
• KOG1
• Mip1
• p150 target of rapamycin (TOR)-scaffold protein
• Raptor
• regulatory associated protein of MTOR, complex 1
• regulatory-associated protein of mTOR
• RPTOR

Background

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPTOR
• Uniprot:
  • Q8N122(Human)