TORC2 Antibody

Western Blot: Torc2 Antibody [NBP2-22356] - Western blot analysis of Torc2 in A. Raji, B. Hek293 and C. Jurkat cell lysates.

Immunocytochemistry/Immunofluorescence: Torc2 Antibody [NBP2-22356] - Torc2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: Torc2 Antibody [NBP2-22356] - IHC staining of Torc2 in mouse thymus.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TORC2 Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human Torc2 protein (between residues 600-693) [UniProt Q53ET0]
• Localization: Cytoplasm and nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRTC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 - 2.0 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: This Torc2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 85 kDa. In ICC/IF cytoplasmic staining was observed in Hela cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Positive Control:

  Jurkat Lysate (NB800-PC2)
  293 Lysate (NB800-PC6)
  Raji Lysate (NB800-PC11)

Reactivity Notes

Human and mouse. Predicted to react in rat and cow based on sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TORC2 Antibody

• CREB regulated transcription coactivator 2
• CREB-regulated transcription coactivator 2
• CRTC2
• TORC2
• TORC-2
• TORC2transducer of regulated cAMP response element-binding protein (CREB) 2
• Transducer of CREB protein 2
• Transducer of regulated cAMP response element-binding protein 2

Background

CREB-regulated transcription coactivator 2 (CRTC2), also known as transducer of regulated cAMP response element-binding protein 2, Torc2, or transducer of CREB protein 2, is a transcriptional coactivator for both CREB and the human T-cell leukemia virus type I (HTLV-1). In its dephosphorylated state, Torc2 becomes an activator in the SIK/TORC signaling pathway, and acts as a central regulator of cAMP dependent gluconeogenic gene expression in the LKB1/AMPK/TORC2 pathway. While Torc2 is normally found in the nucleus, it is translocated to the cytoplasm upon phosphorylation. Torc2 is abundantly expressed in the thymus but can also be found in the spleen, ovary, lung and muscle (where it acts as an inducer of mitochondrial biogenesis). Current research suggests that Torc2 responds to plasma membrane stress by facilitating changes in cellular lipid synthesis, thus altering the composition of the plasma membrane (PMID: 22504275). Other research indicates that Torc2 plays a role in regulating cancer cell migration, invasion, and metastasis (PMID: 21339740).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRTC2
• Entrez:
  • 200186(Human)
  • 74343(Mouse)
  • 310615(Rat)
• Uniprot:
  • Q3U182()
  • Q53ET0(Human)