TORC2 Antibody

Images

 
Western Blot: Torc2 Antibody [NBP2-22356] - Western blot analysis of Torc2 in A. Raji, B. Hek293 and C. Jurkat cell lysates.
Immunocytochemistry/ Immunofluorescence: TORC2 Antibody [NBP2-22356] - Torc2 Antibody [NBP2-22356] - Torc2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more
Immunohistochemistry: Torc2 Antibody [NBP2-22356] - IHC staining of Torc2 in mouse thymus.
Simple Western: TORC2 Antibody [NBP2-22356] - Lane view shows a specific band for TORC1 at a dilution of 1:100 in Jurkat cell lysate at 200 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TORC2 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human Torc2 protein (between residues 600-693) [UniProt Q53ET0]
Localization
Cytoplasm and nucleus.
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRTC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 - 2.0 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This Torc2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 85 kDa. In ICC/IF cytoplasmic staining was observed in Hela cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Jurkat Whole Cell Lysate
293 Whole Cell Lysate
Raji Whole Cell Lysate

Reactivity Notes

Predicted to react in Rat and Bovine based on sequence identity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TORC2 Antibody

  • CREB regulated transcription coactivator 2
  • CREB-regulated transcription coactivator 2
  • CRTC2
  • TORC2
  • TORC-2
  • TORC2transducer of regulated cAMP response element-binding protein (CREB) 2
  • Transducer of CREB protein 2
  • Transducer of regulated cAMP response element-binding protein 2

Background

CREB-regulated transcription coactivator 2 (CRTC2), also known as transducer of regulated cAMP response element-binding protein 2, Torc2, or transducer of CREB protein 2, is a transcriptional coactivator for both CREB and the human T-cell leukemia virus type I (HTLV-1). In its dephosphorylated state, Torc2 becomes an activator in the SIK/TORC signaling pathway, and acts as a central regulator of cAMP dependent gluconeogenic gene expression in the LKB1/AMPK/TORC2 pathway. While Torc2 is normally found in the nucleus, it is translocated to the cytoplasm upon phosphorylation. Torc2 is abundantly expressed in the thymus but can also be found in the spleen, ovary, lung and muscle (where it acts as an inducer of mitochondrial biogenesis). Current research suggests that Torc2 responds to plasma membrane stress by facilitating changes in cellular lipid synthesis, thus altering the composition of the plasma membrane (PMID: 22504275). Other research indicates that Torc2 plays a role in regulating cancer cell migration, invasion, and metastasis (PMID: 21339740).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TORC2 Antibody (NBP2-22356) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22356AF350
Alexa Fluor 405 NBP2-22356AF405
Alexa Fluor 488 NBP2-22356AF488
Alexa Fluor 532 NBP2-22356AF532
Alexa Fluor 594 NBP2-22356AF594
Alexa Fluor 647 NBP2-22356AF647
Alexa Fluor 700 NBP2-22356AF700
Alexa Fluor 750 NBP2-22356AF750
Biotin NBP2-22356B
DyLight 350 NBP2-22356UV
DyLight 405 NBP2-22356V
DyLight 488 NBP2-22356G
DyLight 550 NBP2-22356R
DyLight 594 NBP2-22356DL594
DyLight 650 NBP2-22356C
DyLight 680 NBP2-22356FR
DyLight 755 NBP2-22356IR
FITC NBP2-22356F
HRP NBP2-22356H
Janelia Fluor 549 NBP2-22356JF549
Janelia Fluor 646 NBP2-22356JF646

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Bioinformatics

Gene Symbol CRTC2
Entrez
Uniprot