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TORC2 Antibody - BSA Free

 
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Western Blot: Torc2 Antibody [NBP2-22356] - Western blot analysis of Torc2 in A. Raji, B. Hek293 and C. Jurkat cell lysates.
Western Blot: Torc2 Antibody [NBP2-22356] - Western blot analysis of Torc2 in A. Raji, B. Hek293 and C. Jurkat cell lysates.
Immunocytochemistry/ Immunofluorescence: TORC2 Antibody [NBP2-22356] - Torc2 Antibody [NBP2-22356] - Torc2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more
Immunohistochemistry: Torc2 Antibody [NBP2-22356] - IHC staining of Torc2 in mouse thymus.
Simple Western: TORC2 Antibody [NBP2-22356] - Lane view shows a specific band for TORC1 at a dilution of 1:100 in Jurkat cell lysate at 200 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22356AF350
Alexa Fluor 405
NBP2-22356AF405
Alexa Fluor 488
NBP2-22356AF488
Alexa Fluor 532
NBP2-22356AF532
Alexa Fluor 594
NBP2-22356AF594
Alexa Fluor 647
NBP2-22356AF647
Alexa Fluor 700
NBP2-22356AF700
Alexa Fluor 750
NBP2-22356AF750
Biotin
NBP2-22356B
DyLight 350
NBP2-22356UV
DyLight 405
NBP2-22356V
DyLight 488
NBP2-22356G
DyLight 550
NBP2-22356R
DyLight 594
NBP2-22356DL594
DyLight 650
NBP2-22356C
DyLight 680
NBP2-22356FR
DyLight 755
NBP2-22356IR
FITC
NBP2-22356F
HRP
NBP2-22356H
Janelia Fluor 549
NBP2-22356JF549
Janelia Fluor 646
NBP2-22356JF646
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-22356SS
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TORC2 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human Torc2 protein (between residues 600-693) [UniProt Q53ET0]
Localization
Cytoplasm and nucleus.
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRTC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:100
  • Western Blot 1.0 - 2.0 ug/ml
Application Notes
This Torc2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 85 kDa. In ICC/IF cytoplasmic staining was observed in Hela cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Jurkat Whole Cell Lysate
293 Whole Cell Lysate
Raji Whole Cell Lysate

Reactivity Notes

Predicted to react in Rat and Bovine based on sequence identity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TORC2 Antibody - BSA Free

  • CREB regulated transcription coactivator 2
  • CREB-regulated transcription coactivator 2
  • CRTC2
  • TORC2
  • TORC-2
  • TORC2transducer of regulated cAMP response element-binding protein (CREB) 2
  • Transducer of CREB protein 2
  • Transducer of regulated cAMP response element-binding protein 2

Background

CREB-regulated transcription coactivator 2 (CRTC2), also known as transducer of regulated cAMP response element-binding protein 2, Torc2, or transducer of CREB protein 2, is a transcriptional coactivator for both CREB and the human T-cell leukemia virus type I (HTLV-1). In its dephosphorylated state, Torc2 becomes an activator in the SIK/TORC signaling pathway, and acts as a central regulator of cAMP dependent gluconeogenic gene expression in the LKB1/AMPK/TORC2 pathway. While Torc2 is normally found in the nucleus, it is translocated to the cytoplasm upon phosphorylation. Torc2 is abundantly expressed in the thymus but can also be found in the spleen, ovary, lung and muscle (where it acts as an inducer of mitochondrial biogenesis). Current research suggests that Torc2 responds to plasma membrane stress by facilitating changes in cellular lipid synthesis, thus altering the composition of the plasma membrane (PMID: 22504275). Other research indicates that Torc2 plays a role in regulating cancer cell migration, invasion, and metastasis (PMID: 21339740).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunohistochemistry-Paraffin: TORC1 Antibody [NBP1-89865] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: TORC1 Antibody [NBP1-89865] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.
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[Biotin]
DYC2510-2
A lysate prepared from HeLa human cervical epithelial carcinoma cells treated with 200 nM PMA for 20 minutes was analyzed with this DuoSet IC ELISA. The Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC Detection Antibody was either untreated (no peptide) or pre-incubated with a phosphopeptide containing the CREB S133 phosphorylation site, a phosphopeptide containing the GSK-3 beta S9 phosphorylation site, a phosphopeptide containing the c-Jun S63 phosphorylation site, or a phosphopeptide containing the Raf1 S338 phosphorylation site. Peptides were used at 40 ng/mL. Only the phosphopeptide containing the CREB S133 phosphorylation site blocks the signal, indicating that the DuoSet IC ELISA is specific for CREB phosphorylation at S133.
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NBP1-51645
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gamma/RORC/NR1F3 Anti ...
NBP2-24503
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Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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NB200-157
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Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.
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AMPK alpha 1
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NBP2-22127
Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on NIH3T3 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
ELISA: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
(11 Publications)
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AMPK beta 1
Antibody (2C3)
H00005564-M02
Western Blot: AMPK beta 1 Antibody (2C3) [H00005564-M02] - PRKAB1 monoclonal antibody (M02), clone 2C3 Analysis of PRKAB1 expression in HeLa.
Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Analysis of monoclonal antibody to PRKAB1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Detection limit for recombinant GST tagged PRKAB1 is approximately 0.1ng/ml as a capture antibody.
(1 Review)
Species: Hu, Mu
Applications: ELISA, ICC/IF, S-ELISA, WB
p70 S6 Kinase/S6K
[p Thr389/412] Antibo ...
NB600-1049
Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of Phospho-p70 S6 Kinase in 293 cells.
Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Human liver cancer tissue sections
Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of p70 S6 Kinase phosphorylation expression in Insulin treatedJurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide.
(1 Review)
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Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TORC2 Antibody -
BSA Free
NBP2-22356
TORC2 Antibody - BSA Free
Species: Hu, Mu, Rt, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

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Product General Protocols

View specific protocols for TORC2 Antibody (NBP2-22356): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Jurkat Whole Cell Lysate
293 Whole Cell Lysate
Raji Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

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Bioinformatics

Gene Symbol CRTC2
Entrez
Uniprot