RPTOR Antibodies
We offer RPTOR Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunoprecipitation (Negative), Western Blot. Each RPTOR Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RPTOR Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the RPTOR Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RPTOR polyclonal antibodies and browse our RPTOR monoclonal antibody catalog.
Alternate Names for RPTOR Antibodies
anti-RPTOR antibody, anti-KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats antibody, anti-KOG1 antibody, anti-Mip1 antibody, anti-p150 target of rapamycin (TOR)-scaffold protein antibody, anti-RAPTOR antibody, anti-regulatory associated protein of MTOR, complex 1 antibody, anti-regulatory-associated protein of mTOR antibody
12 Results for "rptor" in Primary Antibodies
![Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NBP2-24837-img0005.jpg "Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.")
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-24837] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RPTOR at a 1:100 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24837-img0006.jpg)
![Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-24837] - Analysis of human small intestine using Raptor antibody at 1:100.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunohistochemistry-Paraffin-NBP2-24837-img0004.jpg)
![Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Western-Blot-NBP3-16729-img0004.jpg "Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunofluorescence analysis of NIH-3T3 cells using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Immunocytochemistry-Immunofluorescence-NBP3-16729-img0003.jpg)
![Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunohistochemistry of paraffin-embedded mouse kidney using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Immunohistochemistry-Paraffin-NBP3-16729-img0002.jpg)
![Western Blot: RPTOR Antibody (SR1170) [NBP3-21541] - Western blot analysis using NBP3-21541 in MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21541_rabbit-rptor-mab-sr1170-5720231552180.jpg "Western Blot: RPTOR Antibody (SR1170) [NBP3-21541] - Western blot analysis using NBP3-21541 in MCF-7 cell lysate.")
![Western Blot: RPTOR Antibody [NBP1-77211] - Analysis of RAPTOR expression in Jurkat cell lysate with RAPTOR antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NBP1-77211-img0005.jpg "Western Blot: RPTOR Antibody [NBP1-77211] - Analysis of RAPTOR expression in Jurkat cell lysate with RAPTOR antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211] - Immunofluorescence of RPTOR in L1210 cells with RPTOR antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77211_rabbit-polyclonal-rptor-antibody-2711202420312940.jpg)
![Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211] - Immunocytochemistry of RAPTOR in L1210 cells with RAPTOR antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77211_rabbit-polyclonal-rptor-antibody-2711202419463498.jpg)
![Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-288202412442987.jpg "Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-29820241435299.jpg)
![Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NB100-766-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: RPTOR Antibody [NB100-766] - Detection of human Raptor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Raptor antibody NB100-766 (lot 3) used for IP at 6 ug per reaction. Raptor was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-Raptor antibody NB100-331 For blotting immunoprecipitated Raptor, NB100-766 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunoprecipitation-NB100-766-img0005.jpg)
![Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/RPTOR-Antibody-1B10-Immunohistochemistry-NBP3-15202-img0001.jpg "Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/RPTOR-Antibody-1B10-Immunohistochemistry-NBP3-15202-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-56133] - Staining of human cell line U-2 OS shows localization to lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56133-img0001.jpg "Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-56133] - Staining of human cell line U-2 OS shows localization to lysosomes. Antibody staining is shown in green.")
![Western Blot: RPTOR [p Ser792] Antibody [NBP3-13173] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPTOR (phospho Ser792) antibody (NBP3-13173) diluted at 1:1000.](http://images.novusbio.com/fullsize/RPTOR-[p-Ser792]-Antibody-Western-Blot-NBP3-13173-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPTOR [p Ser792] Antibody [NBP3-13173] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPTOR (phospho Ser792) antibody (NBP3-13173) diluted at 1:1000.")
